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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-31357350-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=31357350&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 31357350,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001286375.2",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGAX",
          "gene_hgnc_id": 6152,
          "hgvs_c": "c.416C>A",
          "hgvs_p": "p.Pro139Gln",
          "transcript": "NM_000887.5",
          "protein_id": "NP_000878.2",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 1163,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 3492,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000268296.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000887.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGAX",
          "gene_hgnc_id": 6152,
          "hgvs_c": "c.416C>A",
          "hgvs_p": "p.Pro139Gln",
          "transcript": "ENST00000268296.9",
          "protein_id": "ENSP00000268296.5",
          "transcript_support_level": 1,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 1163,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 3492,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000887.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000268296.9"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGAX",
          "gene_hgnc_id": 6152,
          "hgvs_c": "c.416C>A",
          "hgvs_p": "p.Pro139Gln",
          "transcript": "ENST00000562522.2",
          "protein_id": "ENSP00000454623.1",
          "transcript_support_level": 1,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 1169,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 3510,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000562522.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGAX",
          "gene_hgnc_id": 6152,
          "hgvs_c": "n.634C>A",
          "hgvs_p": null,
          "transcript": "ENST00000567409.1",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000567409.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGAX",
          "gene_hgnc_id": 6152,
          "hgvs_c": "c.416C>A",
          "hgvs_p": "p.Pro139Gln",
          "transcript": "NM_001286375.2",
          "protein_id": "NP_001273304.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 1169,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 3510,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001286375.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGAX",
          "gene_hgnc_id": 6152,
          "hgvs_c": "c.416C>A",
          "hgvs_p": "p.Pro139Gln",
          "transcript": "ENST00000958326.1",
          "protein_id": "ENSP00000628385.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 1148,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 3447,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000958326.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 27,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGAX",
          "gene_hgnc_id": 6152,
          "hgvs_c": "c.416C>A",
          "hgvs_p": "p.Pro139Gln",
          "transcript": "ENST00000958327.1",
          "protein_id": "ENSP00000628386.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 1047,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 3144,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000958327.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGAX",
          "gene_hgnc_id": 6152,
          "hgvs_c": "c.335C>A",
          "hgvs_p": "p.Pro112Gln",
          "transcript": "ENST00000562918.5",
          "protein_id": "ENSP00000483860.1",
          "transcript_support_level": 2,
          "aa_start": 112,
          "aa_end": null,
          "aa_length": 181,
          "cds_start": 335,
          "cds_end": null,
          "cds_length": 546,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000562918.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 26,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGAX",
          "gene_hgnc_id": 6152,
          "hgvs_c": "c.416C>A",
          "hgvs_p": "p.Pro139Gln",
          "transcript": "XM_047434074.1",
          "protein_id": "XP_047290030.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 979,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 2940,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434074.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGAX",
          "gene_hgnc_id": 6152,
          "hgvs_c": "c.416C>A",
          "hgvs_p": "p.Pro139Gln",
          "transcript": "XM_011545852.2",
          "protein_id": "XP_011544154.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 798,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 2397,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011545852.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGAX",
          "gene_hgnc_id": 6152,
          "hgvs_c": "c.416C>A",
          "hgvs_p": "p.Pro139Gln",
          "transcript": "XM_047434075.1",
          "protein_id": "XP_047290031.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 627,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 1884,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434075.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ITGAX",
          "gene_hgnc_id": 6152,
          "hgvs_c": "c.416C>A",
          "hgvs_p": "p.Pro139Gln",
          "transcript": "XM_011545854.2",
          "protein_id": "XP_011544156.1",
          "transcript_support_level": null,
          "aa_start": 139,
          "aa_end": null,
          "aa_length": 614,
          "cds_start": 416,
          "cds_end": null,
          "cds_length": 1845,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011545854.2"
        }
      ],
      "gene_symbol": "ITGAX",
      "gene_hgnc_id": 6152,
      "dbsnp": "rs770533015",
      "frequency_reference_population": 0.000056792083,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 91,
      "gnomad_exomes_af": 0.0000586154,
      "gnomad_genomes_af": 0.0000394208,
      "gnomad_exomes_ac": 85,
      "gnomad_genomes_ac": 6,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.2700274884700775,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.219,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2025,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.22,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.953,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001286375.2",
          "gene_symbol": "ITGAX",
          "hgnc_id": 6152,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.416C>A",
          "hgvs_p": "p.Pro139Gln"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}