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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-5079078-T-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=5079078&ref=T&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 5079078,
      "ref": "T",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_019109.5",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.877T>C",
          "hgvs_p": "p.Ser293Pro",
          "transcript": "NM_019109.5",
          "protein_id": "NP_061982.3",
          "transcript_support_level": null,
          "aa_start": 293,
          "aa_end": null,
          "aa_length": 464,
          "cds_start": 877,
          "cds_end": null,
          "cds_length": 1395,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000262374.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_019109.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.877T>C",
          "hgvs_p": "p.Ser293Pro",
          "transcript": "ENST00000262374.10",
          "protein_id": "ENSP00000262374.5",
          "transcript_support_level": 1,
          "aa_start": 293,
          "aa_end": null,
          "aa_length": 464,
          "cds_start": 877,
          "cds_end": null,
          "cds_length": 1395,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_019109.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000262374.10"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.544T>C",
          "hgvs_p": "p.Ser182Pro",
          "transcript": "ENST00000588623.5",
          "protein_id": "ENSP00000468118.1",
          "transcript_support_level": 1,
          "aa_start": 182,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 544,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000588623.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "n.*778T>C",
          "hgvs_p": null,
          "transcript": "ENST00000591822.5",
          "protein_id": "ENSP00000467865.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000591822.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "n.*778T>C",
          "hgvs_p": null,
          "transcript": "ENST00000591822.5",
          "protein_id": "ENSP00000467865.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000591822.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.877T>C",
          "hgvs_p": "p.Ser293Pro",
          "transcript": "ENST00000940711.1",
          "protein_id": "ENSP00000610770.1",
          "transcript_support_level": null,
          "aa_start": 293,
          "aa_end": null,
          "aa_length": 475,
          "cds_start": 877,
          "cds_end": null,
          "cds_length": 1428,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940711.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.877T>C",
          "hgvs_p": "p.Ser293Pro",
          "transcript": "ENST00000902497.1",
          "protein_id": "ENSP00000572556.1",
          "transcript_support_level": null,
          "aa_start": 293,
          "aa_end": null,
          "aa_length": 472,
          "cds_start": 877,
          "cds_end": null,
          "cds_length": 1419,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902497.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.868T>C",
          "hgvs_p": "p.Ser290Pro",
          "transcript": "ENST00000940712.1",
          "protein_id": "ENSP00000610771.1",
          "transcript_support_level": null,
          "aa_start": 290,
          "aa_end": null,
          "aa_length": 461,
          "cds_start": 868,
          "cds_end": null,
          "cds_length": 1386,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940712.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.877T>C",
          "hgvs_p": "p.Ser293Pro",
          "transcript": "ENST00000902499.1",
          "protein_id": "ENSP00000572558.1",
          "transcript_support_level": null,
          "aa_start": 293,
          "aa_end": null,
          "aa_length": 444,
          "cds_start": 877,
          "cds_end": null,
          "cds_length": 1335,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902499.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.877T>C",
          "hgvs_p": "p.Ser293Pro",
          "transcript": "ENST00000684335.1",
          "protein_id": "ENSP00000508112.1",
          "transcript_support_level": null,
          "aa_start": 293,
          "aa_end": null,
          "aa_length": 427,
          "cds_start": 877,
          "cds_end": null,
          "cds_length": 1284,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000684335.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.766T>C",
          "hgvs_p": "p.Ser256Pro",
          "transcript": "ENST00000902498.1",
          "protein_id": "ENSP00000572557.1",
          "transcript_support_level": null,
          "aa_start": 256,
          "aa_end": null,
          "aa_length": 427,
          "cds_start": 766,
          "cds_end": null,
          "cds_length": 1284,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902498.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.877T>C",
          "hgvs_p": "p.Ser293Pro",
          "transcript": "ENST00000940713.1",
          "protein_id": "ENSP00000610772.1",
          "transcript_support_level": null,
          "aa_start": 293,
          "aa_end": null,
          "aa_length": 407,
          "cds_start": 877,
          "cds_end": null,
          "cds_length": 1224,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000940713.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.544T>C",
          "hgvs_p": "p.Ser182Pro",
          "transcript": "NM_001330504.2",
          "protein_id": "NP_001317433.1",
          "transcript_support_level": null,
          "aa_start": 182,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 544,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001330504.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.544T>C",
          "hgvs_p": "p.Ser182Pro",
          "transcript": "ENST00000544428.1",
          "protein_id": "ENSP00000440019.1",
          "transcript_support_level": 2,
          "aa_start": 182,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 544,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000544428.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.544T>C",
          "hgvs_p": "p.Ser182Pro",
          "transcript": "ENST00000683739.1",
          "protein_id": "ENSP00000507002.1",
          "transcript_support_level": null,
          "aa_start": 182,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 544,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000683739.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.388T>C",
          "hgvs_p": "p.Ser130Pro",
          "transcript": "ENST00000682985.1",
          "protein_id": "ENSP00000507598.1",
          "transcript_support_level": null,
          "aa_start": 130,
          "aa_end": null,
          "aa_length": 301,
          "cds_start": 388,
          "cds_end": null,
          "cds_length": 906,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000682985.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.283T>C",
          "hgvs_p": "p.Ser95Pro",
          "transcript": "ENST00000682020.1",
          "protein_id": "ENSP00000508075.1",
          "transcript_support_level": null,
          "aa_start": 95,
          "aa_end": null,
          "aa_length": 266,
          "cds_start": 283,
          "cds_end": null,
          "cds_length": 801,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000682020.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 7,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.862+200T>C",
          "hgvs_p": null,
          "transcript": "ENST00000961949.1",
          "protein_id": "ENSP00000632008.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 452,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1359,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000961949.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 7,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.862+200T>C",
          "hgvs_p": null,
          "transcript": "NM_001438123.1",
          "protein_id": "NP_001425052.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 451,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1356,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001438123.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": 7,
          "intron_rank_end": null,
          "gene_symbol": "ALG1",
          "gene_hgnc_id": 18294,
          "hgvs_c": "c.862+200T>C",
          "hgvs_p": null,
          "transcript": "ENST00000684190.1",
          "protein_id": "ENSP00000507554.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 451,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1356,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000684190.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
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      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
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      "gnomad_mito_homoplasmic": null,
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      "computational_score_selected": 0.8776391744613647,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
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      "revel_prediction": "Pathogenic",
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      "bayesdelnoaf_score": 0.27,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 5.298,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
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      "acmg_score": 8,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM1,PM2,PP3_Moderate,PP5_Moderate",
      "acmg_by_gene": [
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            "PP3_Moderate",
            "PP5_Moderate"
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          "verdict": "Likely_pathogenic",
          "transcript": "NM_019109.5",
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          "inheritance_mode": "AR",
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      "clinvar_disease": "ALG1-congenital disorder of glycosylation",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LP:1",
      "phenotype_combined": "ALG1-congenital disorder of glycosylation",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
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  ],
  "message": null
}