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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-56836704-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=56836704&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 56836704,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000308159.10",
      "consequences": [
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP93",
          "gene_hgnc_id": 28958,
          "hgvs_c": "c.1886A>G",
          "hgvs_p": "p.Tyr629Cys",
          "transcript": "NM_014669.5",
          "protein_id": "NP_055484.3",
          "transcript_support_level": null,
          "aa_start": 629,
          "aa_end": null,
          "aa_length": 819,
          "cds_start": 1886,
          "cds_end": null,
          "cds_length": 2460,
          "cdna_start": 1983,
          "cdna_end": null,
          "cdna_length": 8234,
          "mane_select": "ENST00000308159.10",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP93",
          "gene_hgnc_id": 28958,
          "hgvs_c": "c.1886A>G",
          "hgvs_p": "p.Tyr629Cys",
          "transcript": "ENST00000308159.10",
          "protein_id": "ENSP00000310668.5",
          "transcript_support_level": 1,
          "aa_start": 629,
          "aa_end": null,
          "aa_length": 819,
          "cds_start": 1886,
          "cds_end": null,
          "cds_length": 2460,
          "cdna_start": 1983,
          "cdna_end": null,
          "cdna_length": 8234,
          "mane_select": "NM_014669.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP93",
          "gene_hgnc_id": 28958,
          "hgvs_c": "c.1886A>G",
          "hgvs_p": "p.Tyr629Cys",
          "transcript": "ENST00000569842.5",
          "protein_id": "ENSP00000458101.1",
          "transcript_support_level": 5,
          "aa_start": 629,
          "aa_end": null,
          "aa_length": 880,
          "cds_start": 1886,
          "cds_end": null,
          "cds_length": 2643,
          "cdna_start": 1982,
          "cdna_end": null,
          "cdna_length": 2834,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP93",
          "gene_hgnc_id": 28958,
          "hgvs_c": "c.1517A>G",
          "hgvs_p": "p.Tyr506Cys",
          "transcript": "NM_001242795.2",
          "protein_id": "NP_001229724.1",
          "transcript_support_level": null,
          "aa_start": 506,
          "aa_end": null,
          "aa_length": 696,
          "cds_start": 1517,
          "cds_end": null,
          "cds_length": 2091,
          "cdna_start": 1975,
          "cdna_end": null,
          "cdna_length": 8226,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP93",
          "gene_hgnc_id": 28958,
          "hgvs_c": "c.1517A>G",
          "hgvs_p": "p.Tyr506Cys",
          "transcript": "NM_001242796.2",
          "protein_id": "NP_001229725.1",
          "transcript_support_level": null,
          "aa_start": 506,
          "aa_end": null,
          "aa_length": 696,
          "cds_start": 1517,
          "cds_end": null,
          "cds_length": 2091,
          "cdna_start": 2006,
          "cdna_end": null,
          "cdna_length": 2921,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP93",
          "gene_hgnc_id": 28958,
          "hgvs_c": "c.1517A>G",
          "hgvs_p": "p.Tyr506Cys",
          "transcript": "ENST00000542526.5",
          "protein_id": "ENSP00000440235.1",
          "transcript_support_level": 2,
          "aa_start": 506,
          "aa_end": null,
          "aa_length": 696,
          "cds_start": 1517,
          "cds_end": null,
          "cds_length": 2091,
          "cdna_start": 2006,
          "cdna_end": null,
          "cdna_length": 8257,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP93",
          "gene_hgnc_id": 28958,
          "hgvs_c": "c.1517A>G",
          "hgvs_p": "p.Tyr506Cys",
          "transcript": "ENST00000564887.5",
          "protein_id": "ENSP00000458039.1",
          "transcript_support_level": 2,
          "aa_start": 506,
          "aa_end": null,
          "aa_length": 696,
          "cds_start": 1517,
          "cds_end": null,
          "cds_length": 2091,
          "cdna_start": 2146,
          "cdna_end": null,
          "cdna_length": 2875,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP93",
          "gene_hgnc_id": 28958,
          "hgvs_c": "c.80A>G",
          "hgvs_p": "p.Tyr27Cys",
          "transcript": "ENST00000563405.1",
          "protein_id": "ENSP00000458078.1",
          "transcript_support_level": 2,
          "aa_start": 27,
          "aa_end": null,
          "aa_length": 117,
          "cds_start": 80,
          "cds_end": null,
          "cds_length": 354,
          "cdna_start": 80,
          "cdna_end": null,
          "cdna_length": 477,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "Y",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NUP93",
          "gene_hgnc_id": 28958,
          "hgvs_c": "c.1886A>G",
          "hgvs_p": "p.Tyr629Cys",
          "transcript": "XM_005256263.4",
          "protein_id": "XP_005256320.1",
          "transcript_support_level": null,
          "aa_start": 629,
          "aa_end": null,
          "aa_length": 819,
          "cds_start": 1886,
          "cds_end": null,
          "cds_length": 2460,
          "cdna_start": 2026,
          "cdna_end": null,
          "cdna_length": 8277,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "NUP93",
      "gene_hgnc_id": 28958,
      "dbsnp": "rs757674160",
      "frequency_reference_population": 6.8510514e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.85105e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.9617248177528381,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.03999999910593033,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.628,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.4713,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.25,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 6.962,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.04,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 7,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong,PP5",
      "acmg_by_gene": [
        {
          "score": 7,
          "benign_score": 0,
          "pathogenic_score": 7,
          "criteria": [
            "PM2",
            "PP3_Strong",
            "PP5"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000308159.10",
          "gene_symbol": "NUP93",
          "hgnc_id": 28958,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.1886A>G",
          "hgvs_p": "p.Tyr629Cys"
        }
      ],
      "clinvar_disease": " type 12,Nephrotic syndrome",
      "clinvar_classification": "Pathogenic/Likely pathogenic",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "Nephrotic syndrome, type 12|Nephrotic syndrome",
      "pathogenicity_classification_combined": "Pathogenic/Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}