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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-66608342-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=66608342&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 66608342,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_144601.5",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "NM_181553.4",
          "protein_id": "NP_853531.1",
          "transcript_support_level": null,
          "aa_start": 61,
          "aa_end": null,
          "aa_length": 182,
          "cds_start": 181,
          "cds_end": null,
          "cds_length": 549,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000567572.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_181553.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "ENST00000567572.6",
          "protein_id": "ENSP00000455851.1",
          "transcript_support_level": 1,
          "aa_start": 61,
          "aa_end": null,
          "aa_length": 182,
          "cds_start": 181,
          "cds_end": null,
          "cds_length": 549,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_181553.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000567572.6"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "ENST00000361909.8",
          "protein_id": "ENSP00000354579.4",
          "transcript_support_level": 1,
          "aa_start": 61,
          "aa_end": null,
          "aa_length": 182,
          "cds_start": 181,
          "cds_end": null,
          "cds_length": 549,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000361909.8"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.124+3413G>C",
          "hgvs_p": null,
          "transcript": "ENST00000565922.1",
          "protein_id": "ENSP00000456426.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 59,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 180,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000565922.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "NM_001363918.2",
          "protein_id": "NP_001350847.1",
          "transcript_support_level": null,
          "aa_start": 61,
          "aa_end": null,
          "aa_length": 182,
          "cds_start": 181,
          "cds_end": null,
          "cds_length": 549,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001363918.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "NM_001363923.2",
          "protein_id": "NP_001350852.1",
          "transcript_support_level": null,
          "aa_start": 61,
          "aa_end": null,
          "aa_length": 182,
          "cds_start": 181,
          "cds_end": null,
          "cds_length": 549,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001363923.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "NM_144601.5",
          "protein_id": "NP_653202.1",
          "transcript_support_level": null,
          "aa_start": 61,
          "aa_end": null,
          "aa_length": 182,
          "cds_start": 181,
          "cds_end": null,
          "cds_length": 549,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_144601.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "ENST00000424011.6",
          "protein_id": "ENSP00000400482.2",
          "transcript_support_level": 2,
          "aa_start": 61,
          "aa_end": null,
          "aa_length": 182,
          "cds_start": 181,
          "cds_end": null,
          "cds_length": 549,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000424011.6"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "ENST00000562707.5",
          "protein_id": "ENSP00000455758.1",
          "transcript_support_level": 3,
          "aa_start": 61,
          "aa_end": null,
          "aa_length": 182,
          "cds_start": 181,
          "cds_end": null,
          "cds_length": 549,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000562707.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "ENST00000885751.1",
          "protein_id": "ENSP00000555810.1",
          "transcript_support_level": null,
          "aa_start": 61,
          "aa_end": null,
          "aa_length": 182,
          "cds_start": 181,
          "cds_end": null,
          "cds_length": 549,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000885751.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "ENST00000885752.1",
          "protein_id": "ENSP00000555811.1",
          "transcript_support_level": null,
          "aa_start": 61,
          "aa_end": null,
          "aa_length": 182,
          "cds_start": 181,
          "cds_end": null,
          "cds_length": 549,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000885752.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "ENST00000885753.1",
          "protein_id": "ENSP00000555812.1",
          "transcript_support_level": null,
          "aa_start": 61,
          "aa_end": null,
          "aa_length": 182,
          "cds_start": 181,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000885753.1"
        },
        {
          "aa_ref": "A",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "ENST00000967212.1",
          "protein_id": "ENSP00000637271.1",
          "transcript_support_level": null,
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          "aa_length": 182,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "A",
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          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "ENST00000967213.1",
          "protein_id": "ENSP00000637272.1",
          "transcript_support_level": null,
          "aa_start": 61,
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          "cds_start": 181,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        {
          "aa_ref": "A",
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          ],
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          "intron_rank": null,
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          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "ENST00000967214.1",
          "protein_id": "ENSP00000637273.1",
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          "cds_start": 181,
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          "biotype": "protein_coding",
          "feature": "ENST00000967214.1"
        },
        {
          "aa_ref": "A",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
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          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "ENST00000918764.1",
          "protein_id": "ENSP00000588823.1",
          "transcript_support_level": null,
          "aa_start": 61,
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          "cds_start": 181,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        {
          "aa_ref": "A",
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          "canonical": false,
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          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
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          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "ENST00000564060.5",
          "protein_id": "ENSP00000457589.1",
          "transcript_support_level": 2,
          "aa_start": 61,
          "aa_end": null,
          "aa_length": 150,
          "cds_start": 181,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        {
          "aa_ref": "A",
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          "intron_rank": null,
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          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "ENST00000918765.1",
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "A",
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          ],
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          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "ENST00000562357.5",
          "protein_id": "ENSP00000454628.1",
          "transcript_support_level": 4,
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          "aa_length": 121,
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          "cds_length": 367,
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          "biotype": "protein_coding",
          "feature": "ENST00000562357.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CMTM3",
          "gene_hgnc_id": 19174,
          "hgvs_c": "c.181G>C",
          "hgvs_p": "p.Ala61Pro",
          "transcript": "ENST00000563672.5",
          "protein_id": "ENSP00000457451.1",
          "transcript_support_level": 4,
          "aa_start": 61,
          "aa_end": null,
          "aa_length": 115,
          "cds_start": 181,
          "cds_end": null,
          "cds_length": 349,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000563672.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
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      "gnomad_exomes_homalt": 0,
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      "computational_score_selected": 0.7685977220535278,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.25,
      "splice_prediction_selected": "Uncertain_significance",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.338,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.4837,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.2,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.923,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.25,
      "spliceai_max_prediction": "Uncertain_significance",
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      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
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          "criteria": [
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            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_144601.5",
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          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
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        {
          "score": 3,
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            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "XR_933209.3",
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          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}