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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-67657765-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=67657765&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 67657765,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000620761.6",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "c.1295T>C",
          "hgvs_p": "p.Val432Ala",
          "transcript": "NM_001082486.2",
          "protein_id": "NP_001075955.2",
          "transcript_support_level": null,
          "aa_start": 432,
          "aa_end": null,
          "aa_length": 458,
          "cds_start": 1295,
          "cds_end": null,
          "cds_length": 1377,
          "cdna_start": 1335,
          "cdna_end": null,
          "cdna_length": 1511,
          "mane_select": "ENST00000620761.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "c.1295T>C",
          "hgvs_p": "p.Val432Ala",
          "transcript": "ENST00000620761.6",
          "protein_id": "ENSP00000478084.1",
          "transcript_support_level": 1,
          "aa_start": 432,
          "aa_end": null,
          "aa_length": 458,
          "cds_start": 1295,
          "cds_end": null,
          "cds_length": 1377,
          "cdna_start": 1335,
          "cdna_end": null,
          "cdna_length": 1511,
          "mane_select": "NM_001082486.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "c.1313T>C",
          "hgvs_p": "p.Val438Ala",
          "transcript": "ENST00000695659.1",
          "protein_id": "ENSP00000512089.1",
          "transcript_support_level": null,
          "aa_start": 438,
          "aa_end": null,
          "aa_length": 464,
          "cds_start": 1313,
          "cds_end": null,
          "cds_length": 1395,
          "cdna_start": 1353,
          "cdna_end": null,
          "cdna_length": 1467,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "c.1286T>C",
          "hgvs_p": "p.Val429Ala",
          "transcript": "NM_022914.3",
          "protein_id": "NP_075065.3",
          "transcript_support_level": null,
          "aa_start": 429,
          "aa_end": null,
          "aa_length": 455,
          "cds_start": 1286,
          "cds_end": null,
          "cds_length": 1368,
          "cdna_start": 1326,
          "cdna_end": null,
          "cdna_length": 1502,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "c.1286T>C",
          "hgvs_p": "p.Val429Ala",
          "transcript": "ENST00000219251.13",
          "protein_id": "ENSP00000219251.8",
          "transcript_support_level": 2,
          "aa_start": 429,
          "aa_end": null,
          "aa_length": 455,
          "cds_start": 1286,
          "cds_end": null,
          "cds_length": 1368,
          "cdna_start": 1876,
          "cdna_end": null,
          "cdna_length": 2052,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "c.1277T>C",
          "hgvs_p": "p.Val426Ala",
          "transcript": "ENST00000695648.1",
          "protein_id": "ENSP00000512081.1",
          "transcript_support_level": null,
          "aa_start": 426,
          "aa_end": null,
          "aa_length": 452,
          "cds_start": 1277,
          "cds_end": null,
          "cds_length": 1359,
          "cdna_start": 1334,
          "cdna_end": null,
          "cdna_length": 1442,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "c.1295T>C",
          "hgvs_p": "p.Val432Ala",
          "transcript": "ENST00000695734.1",
          "protein_id": "ENSP00000512127.1",
          "transcript_support_level": null,
          "aa_start": 432,
          "aa_end": null,
          "aa_length": 451,
          "cds_start": 1295,
          "cds_end": null,
          "cds_length": 1356,
          "cdna_start": 1335,
          "cdna_end": null,
          "cdna_length": 1528,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "c.1250T>C",
          "hgvs_p": "p.Val417Ala",
          "transcript": "ENST00000695694.1",
          "protein_id": "ENSP00000512105.1",
          "transcript_support_level": null,
          "aa_start": 417,
          "aa_end": null,
          "aa_length": 443,
          "cds_start": 1250,
          "cds_end": null,
          "cds_length": 1332,
          "cdna_start": 1290,
          "cdna_end": null,
          "cdna_length": 1466,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "c.1247T>C",
          "hgvs_p": "p.Val416Ala",
          "transcript": "ENST00000602320.1",
          "protein_id": "ENSP00000473679.2",
          "transcript_support_level": 5,
          "aa_start": 416,
          "aa_end": null,
          "aa_length": 442,
          "cds_start": 1247,
          "cds_end": null,
          "cds_length": 1329,
          "cdna_start": 1266,
          "cdna_end": null,
          "cdna_length": 1408,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "c.1208T>C",
          "hgvs_p": "p.Val403Ala",
          "transcript": "NM_001410884.1",
          "protein_id": "NP_001397813.1",
          "transcript_support_level": null,
          "aa_start": 403,
          "aa_end": null,
          "aa_length": 429,
          "cds_start": 1208,
          "cds_end": null,
          "cds_length": 1290,
          "cdna_start": 1248,
          "cdna_end": null,
          "cdna_length": 1424,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "c.1208T>C",
          "hgvs_p": "p.Val403Ala",
          "transcript": "ENST00000695697.1",
          "protein_id": "ENSP00000512106.1",
          "transcript_support_level": null,
          "aa_start": 403,
          "aa_end": null,
          "aa_length": 429,
          "cds_start": 1208,
          "cds_end": null,
          "cds_length": 1290,
          "cdna_start": 1286,
          "cdna_end": null,
          "cdna_length": 1381,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "c.1118T>C",
          "hgvs_p": "p.Val373Ala",
          "transcript": "ENST00000695658.1",
          "protein_id": "ENSP00000512088.1",
          "transcript_support_level": null,
          "aa_start": 373,
          "aa_end": null,
          "aa_length": 399,
          "cds_start": 1118,
          "cds_end": null,
          "cds_length": 1200,
          "cdna_start": 1158,
          "cdna_end": null,
          "cdna_length": 1313,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "c.961T>C",
          "hgvs_p": "p.Ser321Pro",
          "transcript": "ENST00000602382.6",
          "protein_id": "ENSP00000473313.2",
          "transcript_support_level": 5,
          "aa_start": 321,
          "aa_end": null,
          "aa_length": 334,
          "cds_start": 961,
          "cds_end": null,
          "cds_length": 1005,
          "cdna_start": 1529,
          "cdna_end": null,
          "cdna_length": 1688,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "S",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "c.874T>C",
          "hgvs_p": "p.Ser292Pro",
          "transcript": "ENST00000695733.1",
          "protein_id": "ENSP00000512126.1",
          "transcript_support_level": null,
          "aa_start": 292,
          "aa_end": null,
          "aa_length": 305,
          "cds_start": 874,
          "cds_end": null,
          "cds_length": 918,
          "cdna_start": 914,
          "cdna_end": null,
          "cdna_length": 1090,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "c.734T>C",
          "hgvs_p": "p.Val245Ala",
          "transcript": "ENST00000695732.1",
          "protein_id": "ENSP00000512125.1",
          "transcript_support_level": null,
          "aa_start": 245,
          "aa_end": null,
          "aa_length": 271,
          "cds_start": 734,
          "cds_end": null,
          "cds_length": 816,
          "cdna_start": 774,
          "cdna_end": null,
          "cdna_length": 950,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "n.2294T>C",
          "hgvs_p": null,
          "transcript": "ENST00000602622.5",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2526,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "n.559T>C",
          "hgvs_p": null,
          "transcript": "ENST00000602656.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 714,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "n.2300T>C",
          "hgvs_p": null,
          "transcript": "ENST00000602780.2",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2487,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "n.2215T>C",
          "hgvs_p": null,
          "transcript": "ENST00000602860.6",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2323,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "n.2404T>C",
          "hgvs_p": null,
          "transcript": "ENST00000695641.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2548,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ACD",
          "gene_hgnc_id": 25070,
          "hgvs_c": "n.2255T>C",
          "hgvs_p": null,
          "transcript": "ENST00000695656.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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      "dbsnp": "rs6979",
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      "allele_count_reference_population": 801245,
      "gnomad_exomes_af": 0.486236,
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      "computational_score_selected": 0.0000010911076060438063,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.029999999329447746,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.035,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0756,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.79,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.091,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.03,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "mitotip_score": null,
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      "acmg_score": -20,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6_Very_Strong,BA1",
      "acmg_by_gene": [
        {
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          "benign_score": 20,
          "pathogenic_score": 0,
          "criteria": [
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            "BP6_Very_Strong",
            "BA1"
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          "verdict": "Benign",
          "transcript": "ENST00000620761.6",
          "gene_symbol": "ACD",
          "hgnc_id": 25070,
          "effects": [
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          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.1295T>C",
          "hgvs_p": "p.Val432Ala"
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        {
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          "pathogenic_score": 0,
          "criteria": [
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            "BP6_Very_Strong",
            "BA1"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000334583.11",
          "gene_symbol": "CARMIL2",
          "hgnc_id": 27089,
          "effects": [
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          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.*247A>G",
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      ],
      "clinvar_disease": " autosomal dominant 6,Dyskeratosis congenita,not provided,not specified",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "B:6 O:1",
      "phenotype_combined": "Dyskeratosis congenita, autosomal dominant 6|not provided|not specified",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}