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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-682232-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=682232&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 682232,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000219548.9",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "STUB1",
          "gene_hgnc_id": 11427,
          "hgvs_c": "c.737C>T",
          "hgvs_p": "p.Thr246Met",
          "transcript": "NM_005861.4",
          "protein_id": "NP_005852.2",
          "transcript_support_level": null,
          "aa_start": 246,
          "aa_end": null,
          "aa_length": 303,
          "cds_start": 737,
          "cds_end": null,
          "cds_length": 912,
          "cdna_start": 853,
          "cdna_end": null,
          "cdna_length": 1340,
          "mane_select": "ENST00000219548.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "STUB1",
          "gene_hgnc_id": 11427,
          "hgvs_c": "c.737C>T",
          "hgvs_p": "p.Thr246Met",
          "transcript": "ENST00000219548.9",
          "protein_id": "ENSP00000219548.4",
          "transcript_support_level": 1,
          "aa_start": 246,
          "aa_end": null,
          "aa_length": 303,
          "cds_start": 737,
          "cds_end": null,
          "cds_length": 912,
          "cdna_start": 853,
          "cdna_end": null,
          "cdna_length": 1340,
          "mane_select": "NM_005861.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "STUB1",
          "gene_hgnc_id": 11427,
          "hgvs_c": "c.521C>T",
          "hgvs_p": "p.Thr174Met",
          "transcript": "ENST00000565677.5",
          "protein_id": "ENSP00000457228.1",
          "transcript_support_level": 1,
          "aa_start": 174,
          "aa_end": null,
          "aa_length": 231,
          "cds_start": 521,
          "cds_end": null,
          "cds_length": 696,
          "cdna_start": 1128,
          "cdna_end": null,
          "cdna_length": 1560,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "JMJD8",
          "gene_hgnc_id": 14148,
          "hgvs_c": "n.1644G>A",
          "hgvs_p": null,
          "transcript": "ENST00000567120.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2205,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "JMJD8",
          "gene_hgnc_id": 14148,
          "hgvs_c": "c.*562G>A",
          "hgvs_p": null,
          "transcript": "NM_001005920.4",
          "protein_id": "NP_001005920.3",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 264,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 795,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1934,
          "mane_select": "ENST00000609261.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "JMJD8",
          "gene_hgnc_id": 14148,
          "hgvs_c": "c.*562G>A",
          "hgvs_p": null,
          "transcript": "ENST00000609261.6",
          "protein_id": "ENSP00000477481.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 264,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 795,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1934,
          "mane_select": "NM_001005920.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "STUB1",
          "gene_hgnc_id": 11427,
          "hgvs_c": "c.521C>T",
          "hgvs_p": "p.Thr174Met",
          "transcript": "NM_001293197.2",
          "protein_id": "NP_001280126.1",
          "transcript_support_level": null,
          "aa_start": 174,
          "aa_end": null,
          "aa_length": 231,
          "cds_start": 521,
          "cds_end": null,
          "cds_length": 696,
          "cdna_start": 942,
          "cdna_end": null,
          "cdna_length": 1429,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "STUB1",
          "gene_hgnc_id": 11427,
          "hgvs_c": "c.521C>T",
          "hgvs_p": "p.Thr174Met",
          "transcript": "ENST00000564370.5",
          "protein_id": "ENSP00000456875.1",
          "transcript_support_level": 2,
          "aa_start": 174,
          "aa_end": null,
          "aa_length": 231,
          "cds_start": 521,
          "cds_end": null,
          "cds_length": 696,
          "cdna_start": 693,
          "cdna_end": null,
          "cdna_length": 875,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "STUB1",
          "gene_hgnc_id": 11427,
          "hgvs_c": "c.452C>T",
          "hgvs_p": "p.Thr151Met",
          "transcript": "ENST00000566408.5",
          "protein_id": "ENSP00000457583.1",
          "transcript_support_level": 2,
          "aa_start": 151,
          "aa_end": null,
          "aa_length": 184,
          "cds_start": 452,
          "cds_end": null,
          "cds_length": 555,
          "cdna_start": 454,
          "cdna_end": null,
          "cdna_length": 684,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "T",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "STUB1",
          "gene_hgnc_id": 11427,
          "hgvs_c": "c.296C>T",
          "hgvs_p": "p.Thr99Met",
          "transcript": "ENST00000564316.1",
          "protein_id": "ENSP00000457090.1",
          "transcript_support_level": 3,
          "aa_start": 99,
          "aa_end": null,
          "aa_length": 156,
          "cds_start": 296,
          "cds_end": null,
          "cds_length": 471,
          "cdna_start": 297,
          "cdna_end": null,
          "cdna_length": 729,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "JMJD8",
          "gene_hgnc_id": 14148,
          "hgvs_c": "n.1441G>A",
          "hgvs_p": null,
          "transcript": "ENST00000565302.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1975,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "JMJD8",
          "gene_hgnc_id": 14148,
          "hgvs_c": "n.797G>A",
          "hgvs_p": null,
          "transcript": "ENST00000568313.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 955,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "JMJD8",
          "gene_hgnc_id": 14148,
          "hgvs_c": "n.1465G>A",
          "hgvs_p": null,
          "transcript": "ENST00000568689.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1998,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "STUB1",
          "gene_hgnc_id": 11427,
          "hgvs_c": "n.1450C>T",
          "hgvs_p": null,
          "transcript": "ENST00000569248.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1853,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "JMJD8",
          "gene_hgnc_id": 14148,
          "hgvs_c": "n.1455G>A",
          "hgvs_p": null,
          "transcript": "NR_136650.3",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
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          "cdna_length": 2017,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "JMJD8",
          "gene_hgnc_id": 14148,
          "hgvs_c": "n.1460G>A",
          "hgvs_p": null,
          "transcript": "NR_136651.3",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2022,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "JMJD8",
          "gene_hgnc_id": 14148,
          "hgvs_c": "n.1370G>A",
          "hgvs_p": null,
          "transcript": "NR_136652.3",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1932,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "JMJD8",
          "gene_hgnc_id": 14148,
          "hgvs_c": "c.*596G>A",
          "hgvs_p": null,
          "transcript": "NM_001323918.3",
          "protein_id": "NP_001310847.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 251,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 756,
          "cdna_start": null,
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          "cdna_length": 1929,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "JMJD8",
          "gene_hgnc_id": 14148,
          "hgvs_c": "c.*562G>A",
          "hgvs_p": null,
          "transcript": "NM_001323920.3",
          "protein_id": "NP_001310849.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": -4,
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          "cds_length": 705,
          "cdna_start": null,
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          "cdna_length": 1844,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "JMJD8",
          "gene_hgnc_id": 14148,
          "hgvs_c": "c.*596G>A",
          "hgvs_p": null,
          "transcript": "NM_001323922.3",
          "protein_id": "NP_001310851.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 221,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 666,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1839,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "JMJD8",
          "gene_hgnc_id": 14148,
          "hgvs_c": "c.*562G>A",
          "hgvs_p": null,
          "transcript": "NM_001323919.3",
          "protein_id": "NP_001310848.2",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 219,
          "cds_start": -4,
          "cds_end": null,
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        {
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          "exon_count": 5,
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        {
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          "exon_count": 7,
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          "gene_symbol": "JMJD8",
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          "transcript": "ENST00000569441.5",
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        }
      ],
      "gene_symbol": "STUB1",
      "gene_hgnc_id": 11427,
      "dbsnp": "rs587777343",
      "frequency_reference_population": 0.0000034230022,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 5,
      "gnomad_exomes_af": 0.000003423,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 5,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.928570032119751,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.788,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9926,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.02,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 7.615,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 8,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM1,PM2,PP2,PP3_Moderate,PP5",
      "acmg_by_gene": [
        {
          "score": 8,
          "benign_score": 0,
          "pathogenic_score": 8,
          "criteria": [
            "PM1",
            "PM2",
            "PP2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "ENST00000219548.9",
          "gene_symbol": "STUB1",
          "hgnc_id": 11427,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.737C>T",
          "hgvs_p": "p.Thr246Met"
        },
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PM2",
            "PP3_Moderate",
            "PP5"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000609261.6",
          "gene_symbol": "JMJD8",
          "hgnc_id": 14148,
          "effects": [
            "3_prime_UTR_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.*562G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Autosomal recessive spinocerebellar ataxia 16,Spinocerebellar ataxia 48,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "P:3 US:1",
      "phenotype_combined": "Autosomal recessive spinocerebellar ataxia 16|not provided|Spinocerebellar ataxia 48",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}