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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-68230431-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=68230431&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 68230431,
      "ref": "C",
      "alt": "T",
      "effect": "synonymous_variant",
      "transcript": "NM_001365264.1",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "c.2022G>A",
          "hgvs_p": "p.Thr674Thr",
          "transcript": "NM_024939.3",
          "protein_id": "NP_079215.2",
          "transcript_support_level": null,
          "aa_start": 674,
          "aa_end": null,
          "aa_length": 717,
          "cds_start": 2022,
          "cds_end": null,
          "cds_length": 2154,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000473183.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_024939.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "c.2022G>A",
          "hgvs_p": "p.Thr674Thr",
          "transcript": "ENST00000473183.7",
          "protein_id": "ENSP00000418748.2",
          "transcript_support_level": 1,
          "aa_start": 674,
          "aa_end": null,
          "aa_length": 717,
          "cds_start": 2022,
          "cds_end": null,
          "cds_length": 2154,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_024939.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000473183.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "n.1987G>A",
          "hgvs_p": null,
          "transcript": "ENST00000251366.7",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000251366.7"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "c.2127G>A",
          "hgvs_p": "p.Thr709Thr",
          "transcript": "ENST00000889115.1",
          "protein_id": "ENSP00000559174.1",
          "transcript_support_level": null,
          "aa_start": 709,
          "aa_end": null,
          "aa_length": 752,
          "cds_start": 2127,
          "cds_end": null,
          "cds_length": 2259,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889115.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "c.2052G>A",
          "hgvs_p": "p.Thr684Thr",
          "transcript": "NM_001365264.1",
          "protein_id": "NP_001352193.1",
          "transcript_support_level": null,
          "aa_start": 684,
          "aa_end": null,
          "aa_length": 727,
          "cds_start": 2052,
          "cds_end": null,
          "cds_length": 2184,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001365264.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "c.2052G>A",
          "hgvs_p": "p.Thr684Thr",
          "transcript": "ENST00000565858.5",
          "protein_id": "ENSP00000454554.1",
          "transcript_support_level": 2,
          "aa_start": 684,
          "aa_end": null,
          "aa_length": 727,
          "cds_start": 2052,
          "cds_end": null,
          "cds_length": 2184,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000565858.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "c.2010G>A",
          "hgvs_p": "p.Thr670Thr",
          "transcript": "ENST00000889113.1",
          "protein_id": "ENSP00000559172.1",
          "transcript_support_level": null,
          "aa_start": 670,
          "aa_end": null,
          "aa_length": 713,
          "cds_start": 2010,
          "cds_end": null,
          "cds_length": 2142,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889113.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "c.1998G>A",
          "hgvs_p": "p.Thr666Thr",
          "transcript": "ENST00000889117.1",
          "protein_id": "ENSP00000559176.1",
          "transcript_support_level": null,
          "aa_start": 666,
          "aa_end": null,
          "aa_length": 709,
          "cds_start": 1998,
          "cds_end": null,
          "cds_length": 2130,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889117.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "c.1989G>A",
          "hgvs_p": "p.Thr663Thr",
          "transcript": "ENST00000929965.1",
          "protein_id": "ENSP00000600024.1",
          "transcript_support_level": null,
          "aa_start": 663,
          "aa_end": null,
          "aa_length": 706,
          "cds_start": 1989,
          "cds_end": null,
          "cds_length": 2121,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000929965.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "c.1947G>A",
          "hgvs_p": "p.Thr649Thr",
          "transcript": "ENST00000889116.1",
          "protein_id": "ENSP00000559175.1",
          "transcript_support_level": null,
          "aa_start": 649,
          "aa_end": null,
          "aa_length": 692,
          "cds_start": 1947,
          "cds_end": null,
          "cds_length": 2079,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889116.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "c.1923G>A",
          "hgvs_p": "p.Thr641Thr",
          "transcript": "ENST00000889114.1",
          "protein_id": "ENSP00000559173.1",
          "transcript_support_level": null,
          "aa_start": 641,
          "aa_end": null,
          "aa_length": 684,
          "cds_start": 1923,
          "cds_end": null,
          "cds_length": 2055,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889114.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "c.1911G>A",
          "hgvs_p": "p.Thr637Thr",
          "transcript": "ENST00000889118.1",
          "protein_id": "ENSP00000559177.1",
          "transcript_support_level": null,
          "aa_start": 637,
          "aa_end": null,
          "aa_length": 680,
          "cds_start": 1911,
          "cds_end": null,
          "cds_length": 2043,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000889118.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "c.2019G>A",
          "hgvs_p": "p.Thr673Thr",
          "transcript": "XM_005256153.6",
          "protein_id": "XP_005256210.1",
          "transcript_support_level": null,
          "aa_start": 673,
          "aa_end": null,
          "aa_length": 716,
          "cds_start": 2019,
          "cds_end": null,
          "cds_length": 2151,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005256153.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "c.1644G>A",
          "hgvs_p": "p.Thr548Thr",
          "transcript": "XM_006721273.5",
          "protein_id": "XP_006721336.1",
          "transcript_support_level": null,
          "aa_start": 548,
          "aa_end": null,
          "aa_length": 591,
          "cds_start": 1644,
          "cds_end": null,
          "cds_length": 1776,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006721273.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": 13,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "c.1899-116G>A",
          "hgvs_p": null,
          "transcript": "NM_001365265.1",
          "protein_id": "NP_001352194.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 641,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1926,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001365265.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "n.*919G>A",
          "hgvs_p": null,
          "transcript": "ENST00000566774.1",
          "protein_id": "ENSP00000463237.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000566774.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ESRP2",
          "gene_hgnc_id": 26152,
          "hgvs_c": "n.*919G>A",
          "hgvs_p": null,
          "transcript": "ENST00000566774.1",
          "protein_id": "ENSP00000463237.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000566774.1"
        }
      ],
      "gene_symbol": "ESRP2",
      "gene_hgnc_id": 26152,
      "dbsnp": "rs377157836",
      "frequency_reference_population": 0.0012101224,
      "hom_count_reference_population": 35,
      "allele_count_reference_population": 1952,
      "gnomad_exomes_af": 0.00126302,
      "gnomad_genomes_af": 0.000702681,
      "gnomad_exomes_ac": 1845,
      "gnomad_genomes_ac": 107,
      "gnomad_exomes_homalt": 34,
      "gnomad_genomes_homalt": 1,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.4300000071525574,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.43,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.581,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -12,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Moderate,BP6,BP7,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -12,
          "benign_score": 12,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BP6",
            "BP7",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_001365264.1",
          "gene_symbol": "ESRP2",
          "hgnc_id": 26152,
          "effects": [
            "synonymous_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.2052G>A",
          "hgvs_p": "p.Thr684Thr"
        }
      ],
      "clinvar_disease": "ESRP2-related disorder",
      "clinvar_classification": "Benign",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "ESRP2-related disorder",
      "pathogenicity_classification_combined": "Benign",
      "custom_annotations": null
    }
  ],
  "message": null
}