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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-70664131-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=70664131&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 70664131,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_138383.3",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTSS2",
          "gene_hgnc_id": 25094,
          "hgvs_c": "c.1790C>G",
          "hgvs_p": "p.Thr597Arg",
          "transcript": "NM_138383.3",
          "protein_id": "NP_612392.1",
          "transcript_support_level": null,
          "aa_start": 597,
          "aa_end": null,
          "aa_length": 747,
          "cds_start": 1790,
          "cds_end": null,
          "cds_length": 2244,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000338779.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_138383.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTSS2",
          "gene_hgnc_id": 25094,
          "hgvs_c": "c.1790C>G",
          "hgvs_p": "p.Thr597Arg",
          "transcript": "ENST00000338779.11",
          "protein_id": "ENSP00000341171.6",
          "transcript_support_level": 1,
          "aa_start": 597,
          "aa_end": null,
          "aa_length": 747,
          "cds_start": 1790,
          "cds_end": null,
          "cds_length": 2244,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_138383.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000338779.11"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTSS2",
          "gene_hgnc_id": 25094,
          "hgvs_c": "c.1922C>G",
          "hgvs_p": "p.Thr641Arg",
          "transcript": "XM_011523450.3",
          "protein_id": "XP_011521752.1",
          "transcript_support_level": null,
          "aa_start": 641,
          "aa_end": null,
          "aa_length": 791,
          "cds_start": 1922,
          "cds_end": null,
          "cds_length": 2376,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011523450.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTSS2",
          "gene_hgnc_id": 25094,
          "hgvs_c": "c.1847C>G",
          "hgvs_p": "p.Thr616Arg",
          "transcript": "XM_011523453.4",
          "protein_id": "XP_011521755.3",
          "transcript_support_level": null,
          "aa_start": 616,
          "aa_end": null,
          "aa_length": 766,
          "cds_start": 1847,
          "cds_end": null,
          "cds_length": 2301,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011523453.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTSS2",
          "gene_hgnc_id": 25094,
          "hgvs_c": "c.1838C>G",
          "hgvs_p": "p.Thr613Arg",
          "transcript": "XM_011523454.4",
          "protein_id": "XP_011521756.3",
          "transcript_support_level": null,
          "aa_start": 613,
          "aa_end": null,
          "aa_length": 763,
          "cds_start": 1838,
          "cds_end": null,
          "cds_length": 2292,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011523454.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTSS2",
          "gene_hgnc_id": 25094,
          "hgvs_c": "c.1781C>G",
          "hgvs_p": "p.Thr594Arg",
          "transcript": "XM_047434890.1",
          "protein_id": "XP_047290846.1",
          "transcript_support_level": null,
          "aa_start": 594,
          "aa_end": null,
          "aa_length": 744,
          "cds_start": 1781,
          "cds_end": null,
          "cds_length": 2235,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434890.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTSS2",
          "gene_hgnc_id": 25094,
          "hgvs_c": "c.1751C>G",
          "hgvs_p": "p.Thr584Arg",
          "transcript": "XM_017023879.3",
          "protein_id": "XP_016879368.2",
          "transcript_support_level": null,
          "aa_start": 584,
          "aa_end": null,
          "aa_length": 734,
          "cds_start": 1751,
          "cds_end": null,
          "cds_length": 2205,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023879.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTSS2",
          "gene_hgnc_id": 25094,
          "hgvs_c": "c.1742C>G",
          "hgvs_p": "p.Thr581Arg",
          "transcript": "XM_047434891.1",
          "protein_id": "XP_047290847.1",
          "transcript_support_level": null,
          "aa_start": 581,
          "aa_end": null,
          "aa_length": 731,
          "cds_start": 1742,
          "cds_end": null,
          "cds_length": 2196,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434891.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTSS2",
          "gene_hgnc_id": 25094,
          "hgvs_c": "c.1715C>G",
          "hgvs_p": "p.Thr572Arg",
          "transcript": "XM_017023880.3",
          "protein_id": "XP_016879369.2",
          "transcript_support_level": null,
          "aa_start": 572,
          "aa_end": null,
          "aa_length": 722,
          "cds_start": 1715,
          "cds_end": null,
          "cds_length": 2169,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023880.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTSS2",
          "gene_hgnc_id": 25094,
          "hgvs_c": "c.1706C>G",
          "hgvs_p": "p.Thr569Arg",
          "transcript": "XM_047434892.1",
          "protein_id": "XP_047290848.1",
          "transcript_support_level": null,
          "aa_start": 569,
          "aa_end": null,
          "aa_length": 719,
          "cds_start": 1706,
          "cds_end": null,
          "cds_length": 2160,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434892.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTSS2",
          "gene_hgnc_id": 25094,
          "hgvs_c": "c.1694C>G",
          "hgvs_p": "p.Thr565Arg",
          "transcript": "XM_017023881.3",
          "protein_id": "XP_016879370.2",
          "transcript_support_level": null,
          "aa_start": 565,
          "aa_end": null,
          "aa_length": 715,
          "cds_start": 1694,
          "cds_end": null,
          "cds_length": 2148,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023881.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTSS2",
          "gene_hgnc_id": 25094,
          "hgvs_c": "c.1685C>G",
          "hgvs_p": "p.Thr562Arg",
          "transcript": "XM_005256236.6",
          "protein_id": "XP_005256293.3",
          "transcript_support_level": null,
          "aa_start": 562,
          "aa_end": null,
          "aa_length": 712,
          "cds_start": 1685,
          "cds_end": null,
          "cds_length": 2139,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005256236.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTSS2",
          "gene_hgnc_id": 25094,
          "hgvs_c": "c.1403C>G",
          "hgvs_p": "p.Thr468Arg",
          "transcript": "XM_011523458.3",
          "protein_id": "XP_011521760.1",
          "transcript_support_level": null,
          "aa_start": 468,
          "aa_end": null,
          "aa_length": 618,
          "cds_start": 1403,
          "cds_end": null,
          "cds_length": 1857,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011523458.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTSS2",
          "gene_hgnc_id": 25094,
          "hgvs_c": "c.1250C>G",
          "hgvs_p": "p.Thr417Arg",
          "transcript": "XM_047434893.1",
          "protein_id": "XP_047290849.1",
          "transcript_support_level": null,
          "aa_start": 417,
          "aa_end": null,
          "aa_length": 567,
          "cds_start": 1250,
          "cds_end": null,
          "cds_length": 1704,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434893.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000279569",
          "gene_hgnc_id": null,
          "hgvs_c": "n.2465G>C",
          "hgvs_p": null,
          "transcript": "ENST00000623414.1",
          "protein_id": null,
          "transcript_support_level": 6,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "TEC",
          "feature": "ENST00000623414.1"
        }
      ],
      "gene_symbol": "MTSS2",
      "gene_hgnc_id": 25094,
      "dbsnp": "rs531163149",
      "frequency_reference_population": 0.0000020572152,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 3,
      "gnomad_exomes_af": 0.00000205722,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 3,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.6289079785346985,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.352,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.4781,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.2,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 7.886,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 5,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PM5,PP2",
      "acmg_by_gene": [
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PM2",
            "PM5",
            "PP2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_138383.3",
          "gene_symbol": "MTSS2",
          "hgnc_id": 25094,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.1790C>G",
          "hgvs_p": "p.Thr597Arg"
        },
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000623414.1",
          "gene_symbol": "ENSG00000279569",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.2465G>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}
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