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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-72021201-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=72021201&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "message": null,
  "variants": [
    {
      "acmg_by_gene": [
        {
          "benign_score": 0,
          "criteria": [
            "PM1",
            "PM2",
            "PP3_Moderate",
            "PP5"
          ],
          "effects": [
            "missense_variant"
          ],
          "gene_symbol": "DHODH",
          "hgnc_id": 2867,
          "hgvs_c": "c.595C>T",
          "hgvs_p": "p.Arg199Cys",
          "inheritance_mode": "AR",
          "pathogenic_score": 7,
          "score": 7,
          "transcript": "NM_001361.5",
          "verdict": "Likely_pathogenic"
        }
      ],
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM1,PM2,PP3_Moderate,PP5",
      "acmg_score": 7,
      "allele_count_reference_population": 37,
      "alphamissense_prediction": null,
      "alphamissense_score": 0.1641,
      "alt": "T",
      "apogee2_prediction": null,
      "apogee2_score": null,
      "bayesdelnoaf_prediction": "Pathogenic",
      "bayesdelnoaf_score": 0.38,
      "chr": "16",
      "clinvar_classification": "Pathogenic",
      "clinvar_disease": "Miller syndrome",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "computational_prediction_selected": "Pathogenic",
      "computational_score_selected": 0.8886052370071411,
      "computational_source_selected": "MetaRNN",
      "consequences": [
        {
          "aa_alt": "C",
          "aa_end": null,
          "aa_length": 395,
          "aa_ref": "R",
          "aa_start": 199,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 4669,
          "cdna_start": 616,
          "cds_end": null,
          "cds_length": 1188,
          "cds_start": 595,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 9,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "NM_001361.5",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "c.595C>T",
          "hgvs_p": "p.Arg199Cys",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "ENST00000219240.9",
          "protein_coding": true,
          "protein_id": "NP_001352.2",
          "strand": true,
          "transcript": "NM_001361.5",
          "transcript_support_level": null
        },
        {
          "aa_alt": "C",
          "aa_end": null,
          "aa_length": 395,
          "aa_ref": "R",
          "aa_start": 199,
          "biotype": "protein_coding",
          "canonical": true,
          "cdna_end": null,
          "cdna_length": 4669,
          "cdna_start": 616,
          "cds_end": null,
          "cds_length": 1188,
          "cds_start": 595,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 9,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000219240.9",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "c.595C>T",
          "hgvs_p": "p.Arg199Cys",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "NM_001361.5",
          "protein_coding": true,
          "protein_id": "ENSP00000219240.4",
          "strand": true,
          "transcript": "ENST00000219240.9",
          "transcript_support_level": 1
        },
        {
          "aa_alt": "C",
          "aa_end": null,
          "aa_length": 457,
          "aa_ref": "R",
          "aa_start": 261,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2617,
          "cdna_start": 796,
          "cds_end": null,
          "cds_length": 1374,
          "cds_start": 781,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 11,
          "exon_rank": 7,
          "exon_rank_end": null,
          "feature": "ENST00000894311.1",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "c.781C>T",
          "hgvs_p": "p.Arg261Cys",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000564370.1",
          "strand": true,
          "transcript": "ENST00000894311.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "C",
          "aa_end": null,
          "aa_length": 394,
          "aa_ref": "R",
          "aa_start": 198,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1510,
          "cdna_start": 607,
          "cds_end": null,
          "cds_length": 1185,
          "cds_start": 592,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 9,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000894313.1",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "c.592C>T",
          "hgvs_p": "p.Arg198Cys",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000564372.1",
          "strand": true,
          "transcript": "ENST00000894313.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "C",
          "aa_end": null,
          "aa_length": 393,
          "aa_ref": "R",
          "aa_start": 199,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 4824,
          "cdna_start": 772,
          "cds_end": null,
          "cds_length": 1182,
          "cds_start": 595,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 9,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000572887.5",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "c.595C>T",
          "hgvs_p": "p.Arg199Cys",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000461848.1",
          "strand": true,
          "transcript": "ENST00000572887.5",
          "transcript_support_level": 5
        },
        {
          "aa_alt": "C",
          "aa_end": null,
          "aa_length": 392,
          "aa_ref": "R",
          "aa_start": 199,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2284,
          "cdna_start": 610,
          "cds_end": null,
          "cds_length": 1179,
          "cds_start": 595,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 9,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000894312.1",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "c.595C>T",
          "hgvs_p": "p.Arg199Cys",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000564371.1",
          "strand": true,
          "transcript": "ENST00000894312.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "C",
          "aa_end": null,
          "aa_length": 375,
          "aa_ref": "R",
          "aa_start": 179,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1462,
          "cdna_start": 556,
          "cds_end": null,
          "cds_length": 1128,
          "cds_start": 535,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 9,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000936686.1",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "c.535C>T",
          "hgvs_p": "p.Arg179Cys",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000606745.1",
          "strand": true,
          "transcript": "ENST00000936686.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "C",
          "aa_end": null,
          "aa_length": 357,
          "aa_ref": "R",
          "aa_start": 199,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2185,
          "cdna_start": 615,
          "cds_end": null,
          "cds_length": 1074,
          "cds_start": 595,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 8,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000936684.1",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "c.595C>T",
          "hgvs_p": "p.Arg199Cys",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000606743.1",
          "strand": true,
          "transcript": "ENST00000936684.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "C",
          "aa_end": null,
          "aa_length": 324,
          "aa_ref": "R",
          "aa_start": 128,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1850,
          "cdna_start": 403,
          "cds_end": null,
          "cds_length": 975,
          "cds_start": 382,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 8,
          "exon_rank": 4,
          "exon_rank_end": null,
          "feature": "ENST00000936685.1",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "c.382C>T",
          "hgvs_p": "p.Arg128Cys",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000606744.1",
          "strand": true,
          "transcript": "ENST00000936685.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "C",
          "aa_end": null,
          "aa_length": 367,
          "aa_ref": "R",
          "aa_start": 171,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 4793,
          "cdna_start": 740,
          "cds_end": null,
          "cds_length": 1104,
          "cds_start": 511,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 9,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "XM_047433674.1",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "c.511C>T",
          "hgvs_p": "p.Arg171Cys",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_047289630.1",
          "strand": true,
          "transcript": "XM_047433674.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "C",
          "aa_end": null,
          "aa_length": 252,
          "aa_ref": "R",
          "aa_start": 56,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 4342,
          "cdna_start": 289,
          "cds_end": null,
          "cds_length": 759,
          "cds_start": 166,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 7,
          "exon_rank": 3,
          "exon_rank_end": null,
          "feature": "XM_005255829.5",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "c.166C>T",
          "hgvs_p": "p.Arg56Cys",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "XP_005255886.1",
          "strand": true,
          "transcript": "XM_005255829.5",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 192,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 607,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 579,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 5,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000574309.5",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "c.512-2944C>T",
          "hgvs_p": null,
          "intron_rank": 4,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000460966.1",
          "strand": true,
          "transcript": "ENST00000574309.5",
          "transcript_support_level": 5
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 162,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1603,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 489,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 4,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000958705.1",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "c.435-2944C>T",
          "hgvs_p": null,
          "intron_rank": 3,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000628764.1",
          "strand": true,
          "transcript": "ENST00000958705.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "retained_intron",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 542,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 4,
          "exon_rank": 4,
          "exon_rank_end": null,
          "feature": "ENST00000572003.5",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "n.512C>T",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": true,
          "transcript": "ENST00000572003.5",
          "transcript_support_level": 4
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "retained_intron",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 2165,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 4,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000571392.1",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "n.1485-1964C>T",
          "hgvs_p": null,
          "intron_rank": 1,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": true,
          "transcript": "ENST00000571392.1",
          "transcript_support_level": 5
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "pseudogene",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 427,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "intron_variant"
          ],
          "exon_count": 4,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000573922.5",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "n.314-1964C>T",
          "hgvs_p": null,
          "intron_rank": 3,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": true,
          "transcript": "ENST00000573922.5",
          "transcript_support_level": 5
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "retained_intron",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 549,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_count": 4,
          "exon_rank": null,
          "exon_rank_end": null,
          "feature": "ENST00000573843.1",
          "gene_hgnc_id": 2867,
          "gene_symbol": "DHODH",
          "hgvs_c": "n.*9C>T",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": true,
          "transcript": "ENST00000573843.1",
          "transcript_support_level": 4
        }
      ],
      "custom_annotations": null,
      "dbscsnv_ada_prediction": null,
      "dbscsnv_ada_score": null,
      "dbsnp": "rs267606769",
      "effect": "missense_variant",
      "frequency_reference_population": 0.000022972206,
      "gene_hgnc_id": 2867,
      "gene_symbol": "DHODH",
      "gnomad_exomes_ac": 32,
      "gnomad_exomes_af": 0.0000219411,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_ac": 5,
      "gnomad_genomes_af": 0.0000328528,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_heteroplasmic": null,
      "gnomad_mito_homoplasmic": null,
      "hom_count_reference_population": 0,
      "mitotip_prediction": null,
      "mitotip_score": null,
      "pathogenicity_classification_combined": "Pathogenic",
      "phenotype_combined": "Miller syndrome",
      "phylop100way_prediction": "Uncertain_significance",
      "phylop100way_score": 4.806,
      "pos": 72021201,
      "ref": "C",
      "revel_prediction": "Pathogenic",
      "revel_score": 0.659,
      "splice_prediction_selected": "Benign",
      "splice_score_selected": 0,
      "splice_source_selected": "max_spliceai",
      "spliceai_max_prediction": "Benign",
      "spliceai_max_score": 0,
      "transcript": "NM_001361.5"
    }
  ]
}
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