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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-75234923-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=75234923&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 75234923,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001170714.3",
      "consequences": [
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.1976G>C",
          "hgvs_p": "p.Gly659Ala",
          "transcript": "NM_014567.5",
          "protein_id": "NP_055382.2",
          "transcript_support_level": null,
          "aa_start": 659,
          "aa_end": null,
          "aa_length": 870,
          "cds_start": 1976,
          "cds_end": null,
          "cds_length": 2613,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000162330.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_014567.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.1976G>C",
          "hgvs_p": "p.Gly659Ala",
          "transcript": "ENST00000162330.10",
          "protein_id": "ENSP00000162330.5",
          "transcript_support_level": 1,
          "aa_start": 659,
          "aa_end": null,
          "aa_length": 870,
          "cds_start": 1976,
          "cds_end": null,
          "cds_length": 2613,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_014567.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000162330.10"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "n.1581G>C",
          "hgvs_p": null,
          "transcript": "ENST00000563038.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000563038.5"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.2114G>C",
          "hgvs_p": "p.Gly705Ala",
          "transcript": "NM_001170714.3",
          "protein_id": "NP_001164185.1",
          "transcript_support_level": null,
          "aa_start": 705,
          "aa_end": null,
          "aa_length": 916,
          "cds_start": 2114,
          "cds_end": null,
          "cds_length": 2751,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001170714.3"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.2114G>C",
          "hgvs_p": "p.Gly705Ala",
          "transcript": "ENST00000418647.7",
          "protein_id": "ENSP00000391669.3",
          "transcript_support_level": 2,
          "aa_start": 705,
          "aa_end": null,
          "aa_length": 916,
          "cds_start": 2114,
          "cds_end": null,
          "cds_length": 2751,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000418647.7"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.1976G>C",
          "hgvs_p": "p.Gly659Ala",
          "transcript": "ENST00000853597.1",
          "protein_id": "ENSP00000523656.1",
          "transcript_support_level": null,
          "aa_start": 659,
          "aa_end": null,
          "aa_length": 891,
          "cds_start": 1976,
          "cds_end": null,
          "cds_length": 2676,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000853597.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.2030G>C",
          "hgvs_p": "p.Gly677Ala",
          "transcript": "NM_001170715.3",
          "protein_id": "NP_001164186.1",
          "transcript_support_level": null,
          "aa_start": 677,
          "aa_end": null,
          "aa_length": 888,
          "cds_start": 2030,
          "cds_end": null,
          "cds_length": 2667,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001170715.3"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.2030G>C",
          "hgvs_p": "p.Gly677Ala",
          "transcript": "NM_001170716.3",
          "protein_id": "NP_001164187.1",
          "transcript_support_level": null,
          "aa_start": 677,
          "aa_end": null,
          "aa_length": 888,
          "cds_start": 2030,
          "cds_end": null,
          "cds_length": 2667,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001170716.3"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.2030G>C",
          "hgvs_p": "p.Gly677Ala",
          "transcript": "NM_001170717.3",
          "protein_id": "NP_001164188.1",
          "transcript_support_level": null,
          "aa_start": 677,
          "aa_end": null,
          "aa_length": 888,
          "cds_start": 2030,
          "cds_end": null,
          "cds_length": 2667,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001170717.3"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.2030G>C",
          "hgvs_p": "p.Gly677Ala",
          "transcript": "ENST00000393420.10",
          "protein_id": "ENSP00000377072.6",
          "transcript_support_level": 5,
          "aa_start": 677,
          "aa_end": null,
          "aa_length": 888,
          "cds_start": 2030,
          "cds_end": null,
          "cds_length": 2667,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000393420.10"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.2030G>C",
          "hgvs_p": "p.Gly677Ala",
          "transcript": "ENST00000393422.6",
          "protein_id": "ENSP00000377074.2",
          "transcript_support_level": 2,
          "aa_start": 677,
          "aa_end": null,
          "aa_length": 888,
          "cds_start": 2030,
          "cds_end": null,
          "cds_length": 2667,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000393422.6"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.2030G>C",
          "hgvs_p": "p.Gly677Ala",
          "transcript": "ENST00000420641.7",
          "protein_id": "ENSP00000392708.3",
          "transcript_support_level": 2,
          "aa_start": 677,
          "aa_end": null,
          "aa_length": 888,
          "cds_start": 2030,
          "cds_end": null,
          "cds_length": 2667,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000420641.7"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.1994G>C",
          "hgvs_p": "p.Gly665Ala",
          "transcript": "ENST00000942293.1",
          "protein_id": "ENSP00000612352.1",
          "transcript_support_level": null,
          "aa_start": 665,
          "aa_end": null,
          "aa_length": 876,
          "cds_start": 1994,
          "cds_end": null,
          "cds_length": 2631,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942293.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.1976G>C",
          "hgvs_p": "p.Gly659Ala",
          "transcript": "NM_001170718.3",
          "protein_id": "NP_001164189.1",
          "transcript_support_level": null,
          "aa_start": 659,
          "aa_end": null,
          "aa_length": 870,
          "cds_start": 1976,
          "cds_end": null,
          "cds_length": 2613,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001170718.3"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.1976G>C",
          "hgvs_p": "p.Gly659Ala",
          "transcript": "ENST00000538440.6",
          "protein_id": "ENSP00000443841.2",
          "transcript_support_level": 2,
          "aa_start": 659,
          "aa_end": null,
          "aa_length": 870,
          "cds_start": 1976,
          "cds_end": null,
          "cds_length": 2613,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000538440.6"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.1970G>C",
          "hgvs_p": "p.Gly657Ala",
          "transcript": "NM_001170719.3",
          "protein_id": "NP_001164190.1",
          "transcript_support_level": null,
          "aa_start": 657,
          "aa_end": null,
          "aa_length": 868,
          "cds_start": 1970,
          "cds_end": null,
          "cds_length": 2607,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001170719.3"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.1970G>C",
          "hgvs_p": "p.Gly657Ala",
          "transcript": "ENST00000542031.6",
          "protein_id": "ENSP00000440415.2",
          "transcript_support_level": 2,
          "aa_start": 657,
          "aa_end": null,
          "aa_length": 868,
          "cds_start": 1970,
          "cds_end": null,
          "cds_length": 2607,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000542031.6"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.1976G>C",
          "hgvs_p": "p.Gly659Ala",
          "transcript": "ENST00000928349.1",
          "protein_id": "ENSP00000598408.1",
          "transcript_support_level": null,
          "aa_start": 659,
          "aa_end": null,
          "aa_length": 868,
          "cds_start": 1976,
          "cds_end": null,
          "cds_length": 2607,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000928349.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.1976G>C",
          "hgvs_p": "p.Gly659Ala",
          "transcript": "ENST00000942295.1",
          "protein_id": "ENSP00000612354.1",
          "transcript_support_level": null,
          "aa_start": 659,
          "aa_end": null,
          "aa_length": 867,
          "cds_start": 1976,
          "cds_end": null,
          "cds_length": 2604,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942295.1"
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "BCAR1",
          "gene_hgnc_id": 971,
          "hgvs_c": "c.1883G>C",
          "hgvs_p": "p.Gly628Ala",
          "transcript": "ENST00000942294.1",
          "protein_id": "ENSP00000612353.1",
          "transcript_support_level": null,
          "aa_start": 628,
          "aa_end": null,
          "aa_length": 839,
          "cds_start": 1883,
          "cds_end": null,
          "cds_length": 2520,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
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        {
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        {
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          "consequences": [
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          ],
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          "transcript": "ENST00000562556.5",
          "protein_id": "ENSP00000455166.1",
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000562556.5"
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      ],
      "gene_symbol": "BCAR1",
      "gene_hgnc_id": 971,
      "dbsnp": "rs1232194704",
      "frequency_reference_population": 7.1379526e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 7.13795e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.46471476554870605,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.177,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1595,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.17,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.841,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
            "PM2"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001170714.3",
          "gene_symbol": "BCAR1",
          "hgnc_id": 971,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.2114G>C",
          "hgvs_p": "p.Gly705Ala"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}