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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-7709066-G-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=7709066&ref=G&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 7709066,
      "ref": "G",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000550418.6",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1006G>T",
          "hgvs_p": "p.Val336Phe",
          "transcript": "NM_018723.4",
          "protein_id": "NP_061193.2",
          "transcript_support_level": null,
          "aa_start": 336,
          "aa_end": null,
          "aa_length": 397,
          "cds_start": 1006,
          "cds_end": null,
          "cds_length": 1194,
          "cdna_start": 2101,
          "cdna_end": null,
          "cdna_length": 4884,
          "mane_select": "ENST00000550418.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "F",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1006G>T",
          "hgvs_p": "p.Val336Phe",
          "transcript": "ENST00000550418.6",
          "protein_id": "ENSP00000450031.1",
          "transcript_support_level": 1,
          "aa_start": 336,
          "aa_end": null,
          "aa_length": 397,
          "cds_start": 1006,
          "cds_end": null,
          "cds_length": 1194,
          "cdna_start": 2101,
          "cdna_end": null,
          "cdna_length": 4884,
          "mane_select": "NM_018723.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1122G>T",
          "hgvs_p": "p.Glu374Asp",
          "transcript": "NM_145893.3",
          "protein_id": "NP_665900.1",
          "transcript_support_level": null,
          "aa_start": 374,
          "aa_end": null,
          "aa_length": 395,
          "cds_start": 1122,
          "cds_end": null,
          "cds_length": 1188,
          "cdna_start": 1279,
          "cdna_end": null,
          "cdna_length": 4062,
          "mane_select": null,
          "mane_plus": "ENST00000355637.9",
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1122G>T",
          "hgvs_p": "p.Glu374Asp",
          "transcript": "ENST00000355637.9",
          "protein_id": "ENSP00000347855.4",
          "transcript_support_level": 1,
          "aa_start": 374,
          "aa_end": null,
          "aa_length": 395,
          "cds_start": 1122,
          "cds_end": null,
          "cds_length": 1188,
          "cdna_start": 1279,
          "cdna_end": null,
          "cdna_length": 4062,
          "mane_select": null,
          "mane_plus": "NM_145893.3",
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1069G>T",
          "hgvs_p": "p.Val357Phe",
          "transcript": "ENST00000311745.9",
          "protein_id": "ENSP00000309117.5",
          "transcript_support_level": 1,
          "aa_start": 357,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 1069,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": 1321,
          "cdna_end": null,
          "cdna_length": 3268,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1069G>T",
          "hgvs_p": "p.Val357Phe",
          "transcript": "ENST00000436368.6",
          "protein_id": "ENSP00000402745.2",
          "transcript_support_level": 1,
          "aa_start": 357,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 1069,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": 1321,
          "cdna_end": null,
          "cdna_length": 1530,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.925G>T",
          "hgvs_p": "p.Val309Phe",
          "transcript": "ENST00000553186.5",
          "protein_id": "ENSP00000447753.1",
          "transcript_support_level": 1,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 370,
          "cds_start": 925,
          "cds_end": null,
          "cds_length": 1113,
          "cdna_start": 1913,
          "cdna_end": null,
          "cdna_length": 2196,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1536G>T",
          "hgvs_p": "p.Glu512Asp",
          "transcript": "ENST00000641259.1",
          "protein_id": "ENSP00000493041.1",
          "transcript_support_level": null,
          "aa_start": 512,
          "aa_end": null,
          "aa_length": 582,
          "cds_start": 1536,
          "cds_end": null,
          "cds_length": 1749,
          "cdna_start": 1621,
          "cdna_end": null,
          "cdna_length": 3651,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1656G>T",
          "hgvs_p": "p.Glu552Asp",
          "transcript": "NM_001415887.1",
          "protein_id": "NP_001402816.1",
          "transcript_support_level": null,
          "aa_start": 552,
          "aa_end": null,
          "aa_length": 573,
          "cds_start": 1656,
          "cds_end": null,
          "cds_length": 1722,
          "cdna_start": 1702,
          "cdna_end": null,
          "cdna_length": 4485,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1522G>T",
          "hgvs_p": "p.Val508Phe",
          "transcript": "NM_001415888.1",
          "protein_id": "NP_001402817.1",
          "transcript_support_level": null,
          "aa_start": 508,
          "aa_end": null,
          "aa_length": 569,
          "cds_start": 1522,
          "cds_end": null,
          "cds_length": 1710,
          "cdna_start": 1568,
          "cdna_end": null,
          "cdna_length": 4351,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1188G>T",
          "hgvs_p": "p.Glu396Asp",
          "transcript": "NM_001411047.1",
          "protein_id": "NP_001397976.1",
          "transcript_support_level": null,
          "aa_start": 396,
          "aa_end": null,
          "aa_length": 466,
          "cds_start": 1188,
          "cds_end": null,
          "cds_length": 1401,
          "cdna_start": 2063,
          "cdna_end": null,
          "cdna_length": 2416,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1188G>T",
          "hgvs_p": "p.Glu396Asp",
          "transcript": "ENST00000547372.5",
          "protein_id": "ENSP00000446842.1",
          "transcript_support_level": 5,
          "aa_start": 396,
          "aa_end": null,
          "aa_length": 466,
          "cds_start": 1188,
          "cds_end": null,
          "cds_length": 1401,
          "cdna_start": 1494,
          "cdna_end": null,
          "cdna_length": 3524,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1167G>T",
          "hgvs_p": "p.Glu389Asp",
          "transcript": "NM_001415889.1",
          "protein_id": "NP_001402818.1",
          "transcript_support_level": null,
          "aa_start": 389,
          "aa_end": null,
          "aa_length": 459,
          "cds_start": 1167,
          "cds_end": null,
          "cds_length": 1380,
          "cdna_start": 1281,
          "cdna_end": null,
          "cdna_length": 5193,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "D",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1134G>T",
          "hgvs_p": "p.Glu378Asp",
          "transcript": "NM_001415890.1",
          "protein_id": "NP_001402819.1",
          "transcript_support_level": null,
          "aa_start": 378,
          "aa_end": null,
          "aa_length": 448,
          "cds_start": 1134,
          "cds_end": null,
          "cds_length": 1347,
          "cdna_start": 1162,
          "cdna_end": null,
          "cdna_length": 5074,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1135G>T",
          "hgvs_p": "p.Val379Phe",
          "transcript": "NM_001415891.1",
          "protein_id": "NP_001402820.1",
          "transcript_support_level": null,
          "aa_start": 379,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 1135,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": 2010,
          "cdna_end": null,
          "cdna_length": 4793,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1114G>T",
          "hgvs_p": "p.Val372Phe",
          "transcript": "NM_001415892.1",
          "protein_id": "NP_001402821.1",
          "transcript_support_level": null,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 433,
          "cds_start": 1114,
          "cds_end": null,
          "cds_length": 1302,
          "cdna_start": 1228,
          "cdna_end": null,
          "cdna_length": 4011,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1135G>T",
          "hgvs_p": "p.Val379Phe",
          "transcript": "NM_001308117.1",
          "protein_id": "NP_001295046.1",
          "transcript_support_level": null,
          "aa_start": 379,
          "aa_end": null,
          "aa_length": 425,
          "cds_start": 1135,
          "cds_end": null,
          "cds_length": 1278,
          "cdna_start": 1331,
          "cdna_end": null,
          "cdna_length": 1684,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "F",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
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          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1135G>T",
          "hgvs_p": "p.Val379Phe",
          "transcript": "ENST00000422070.8",
          "protein_id": "ENSP00000391269.4",
          "transcript_support_level": 2,
          "aa_start": 379,
          "aa_end": null,
          "aa_length": 425,
          "cds_start": 1135,
          "cds_end": null,
          "cds_length": 1278,
          "cdna_start": 1331,
          "cdna_end": null,
          "cdna_length": 1684,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1081G>T",
          "hgvs_p": "p.Val361Phe",
          "transcript": "NM_001415893.1",
          "protein_id": "NP_001402822.1",
          "transcript_support_level": null,
          "aa_start": 361,
          "aa_end": null,
          "aa_length": 422,
          "cds_start": 1081,
          "cds_end": null,
          "cds_length": 1269,
          "cdna_start": 1109,
          "cdna_end": null,
          "cdna_length": 3892,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1114G>T",
          "hgvs_p": "p.Val372Phe",
          "transcript": "NM_001415894.1",
          "protein_id": "NP_001402823.1",
          "transcript_support_level": null,
          "aa_start": 372,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 1114,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": 1228,
          "cdna_end": null,
          "cdna_length": 5140,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
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          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 352,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "RBFOX1",
      "gene_hgnc_id": 18222,
      "dbsnp": "rs766496349",
      "frequency_reference_population": 6.842482e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84248e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.31136763095855713,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.313,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.3917,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.14,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.179,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000550418.6",
          "gene_symbol": "RBFOX1",
          "hgnc_id": 18222,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "Unknown,AD",
          "hgvs_c": "c.1006G>T",
          "hgvs_p": "p.Val336Phe"
        },
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000777597.1",
          "gene_symbol": "ENSG00000301273",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.403-15605C>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}