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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-7710640-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=7710640&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 7710640,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "ENST00000550418.6",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1089G>C",
          "hgvs_p": "p.Leu363Phe",
          "transcript": "NM_018723.4",
          "protein_id": "NP_061193.2",
          "transcript_support_level": null,
          "aa_start": 363,
          "aa_end": null,
          "aa_length": 397,
          "cds_start": 1089,
          "cds_end": null,
          "cds_length": 1194,
          "cdna_start": 2184,
          "cdna_end": null,
          "cdna_length": 4884,
          "mane_select": "ENST00000550418.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1089G>C",
          "hgvs_p": "p.Leu363Phe",
          "transcript": "ENST00000550418.6",
          "protein_id": "ENSP00000450031.1",
          "transcript_support_level": 1,
          "aa_start": 363,
          "aa_end": null,
          "aa_length": 397,
          "cds_start": 1089,
          "cds_end": null,
          "cds_length": 1194,
          "cdna_start": 2184,
          "cdna_end": null,
          "cdna_length": 4884,
          "mane_select": "NM_018723.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1152G>C",
          "hgvs_p": "p.Leu384Phe",
          "transcript": "ENST00000311745.9",
          "protein_id": "ENSP00000309117.5",
          "transcript_support_level": 1,
          "aa_start": 384,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 1152,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": 1404,
          "cdna_end": null,
          "cdna_length": 3268,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1008G>C",
          "hgvs_p": "p.Leu336Phe",
          "transcript": "ENST00000553186.5",
          "protein_id": "ENSP00000447753.1",
          "transcript_support_level": 1,
          "aa_start": 336,
          "aa_end": null,
          "aa_length": 370,
          "cds_start": 1008,
          "cds_end": null,
          "cds_length": 1113,
          "cdna_start": 1996,
          "cdna_end": null,
          "cdna_length": 2196,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.*17G>C",
          "hgvs_p": null,
          "transcript": "NM_145893.3",
          "protein_id": "NP_665900.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 395,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1188,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4062,
          "mane_select": null,
          "mane_plus": "ENST00000355637.9",
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.*17G>C",
          "hgvs_p": null,
          "transcript": "ENST00000355637.9",
          "protein_id": "ENSP00000347855.4",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 395,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1188,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4062,
          "mane_select": null,
          "mane_plus": "NM_145893.3",
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": 12,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1135-61G>C",
          "hgvs_p": null,
          "transcript": "ENST00000436368.6",
          "protein_id": "ENSP00000402745.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1530,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1605G>C",
          "hgvs_p": "p.Leu535Phe",
          "transcript": "NM_001415888.1",
          "protein_id": "NP_001402817.1",
          "transcript_support_level": null,
          "aa_start": 535,
          "aa_end": null,
          "aa_length": 569,
          "cds_start": 1605,
          "cds_end": null,
          "cds_length": 1710,
          "cdna_start": 1651,
          "cdna_end": null,
          "cdna_length": 4351,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1218G>C",
          "hgvs_p": "p.Leu406Phe",
          "transcript": "NM_001415891.1",
          "protein_id": "NP_001402820.1",
          "transcript_support_level": null,
          "aa_start": 406,
          "aa_end": null,
          "aa_length": 440,
          "cds_start": 1218,
          "cds_end": null,
          "cds_length": 1323,
          "cdna_start": 2093,
          "cdna_end": null,
          "cdna_length": 4793,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1197G>C",
          "hgvs_p": "p.Leu399Phe",
          "transcript": "NM_001415892.1",
          "protein_id": "NP_001402821.1",
          "transcript_support_level": null,
          "aa_start": 399,
          "aa_end": null,
          "aa_length": 433,
          "cds_start": 1197,
          "cds_end": null,
          "cds_length": 1302,
          "cdna_start": 1311,
          "cdna_end": null,
          "cdna_length": 4011,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1164G>C",
          "hgvs_p": "p.Leu388Phe",
          "transcript": "NM_001415893.1",
          "protein_id": "NP_001402822.1",
          "transcript_support_level": null,
          "aa_start": 388,
          "aa_end": null,
          "aa_length": 422,
          "cds_start": 1164,
          "cds_end": null,
          "cds_length": 1269,
          "cdna_start": 1192,
          "cdna_end": null,
          "cdna_length": 3892,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1152G>C",
          "hgvs_p": "p.Leu384Phe",
          "transcript": "NM_145891.3",
          "protein_id": "NP_665898.1",
          "transcript_support_level": null,
          "aa_start": 384,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 1152,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": 1309,
          "cdna_end": null,
          "cdna_length": 4009,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1149G>C",
          "hgvs_p": "p.Leu383Phe",
          "transcript": "NM_001415896.1",
          "protein_id": "NP_001402825.1",
          "transcript_support_level": null,
          "aa_start": 383,
          "aa_end": null,
          "aa_length": 417,
          "cds_start": 1149,
          "cds_end": null,
          "cds_length": 1254,
          "cdna_start": 1306,
          "cdna_end": null,
          "cdna_length": 4006,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1137G>C",
          "hgvs_p": "p.Leu379Phe",
          "transcript": "NM_001415897.1",
          "protein_id": "NP_001402826.1",
          "transcript_support_level": null,
          "aa_start": 379,
          "aa_end": null,
          "aa_length": 413,
          "cds_start": 1137,
          "cds_end": null,
          "cds_length": 1242,
          "cdna_start": 2012,
          "cdna_end": null,
          "cdna_length": 4712,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
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          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1116G>C",
          "hgvs_p": "p.Leu372Phe",
          "transcript": "NM_001415900.1",
          "protein_id": "NP_001402829.1",
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          "aa_start": 372,
          "aa_end": null,
          "aa_length": 406,
          "cds_start": 1116,
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          "cds_length": 1221,
          "cdna_start": 1230,
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          "cdna_length": 3930,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1095G>C",
          "hgvs_p": "p.Leu365Phe",
          "transcript": "NM_001415902.1",
          "protein_id": "NP_001402831.1",
          "transcript_support_level": null,
          "aa_start": 365,
          "aa_end": null,
          "aa_length": 399,
          "cds_start": 1095,
          "cds_end": null,
          "cds_length": 1200,
          "cdna_start": 1253,
          "cdna_end": null,
          "cdna_length": 3953,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1089G>C",
          "hgvs_p": "p.Leu363Phe",
          "transcript": "NM_001142334.2",
          "protein_id": "NP_001135806.1",
          "transcript_support_level": null,
          "aa_start": 363,
          "aa_end": null,
          "aa_length": 397,
          "cds_start": 1089,
          "cds_end": null,
          "cds_length": 1194,
          "cdna_start": 1309,
          "cdna_end": null,
          "cdna_length": 4009,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1089G>C",
          "hgvs_p": "p.Leu363Phe",
          "transcript": "ENST00000547338.5",
          "protein_id": "ENSP00000447717.1",
          "transcript_support_level": 2,
          "aa_start": 363,
          "aa_end": null,
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          "cds_start": 1089,
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          "cdna_start": 1309,
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          "cdna_length": 1513,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1089G>C",
          "hgvs_p": "p.Leu363Phe",
          "transcript": "ENST00000675653.1",
          "protein_id": "ENSP00000502718.1",
          "transcript_support_level": null,
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          "aa_end": null,
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          "cds_start": 1089,
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          "cdna_start": 1334,
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          "cdna_length": 4019,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RBFOX1",
          "gene_hgnc_id": 18222,
          "hgvs_c": "c.1089G>C",
          "hgvs_p": "p.Leu363Phe",
          "transcript": "ENST00000675842.1",
          "protein_id": "ENSP00000501599.1",
          "transcript_support_level": null,
          "aa_start": 363,
          "aa_end": null,
          "aa_length": 397,
          "cds_start": 1089,
          "cds_end": null,
          "cds_length": 1194,
          "cdna_start": 1569,
          "cdna_end": null,
          "cdna_length": 4254,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "F",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
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      ],
      "gene_symbol": "RBFOX1",
      "gene_hgnc_id": 18222,
      "dbsnp": "rs776822373",
      "frequency_reference_population": 6.8442426e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84424e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.19993039965629578,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.07000000029802322,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.143,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2408,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.32,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 6.655,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.07,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
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      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000550418.6",
          "gene_symbol": "RBFOX1",
          "hgnc_id": 18222,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "Unknown,AD",
          "hgvs_c": "c.1089G>C",
          "hgvs_p": "p.Leu363Phe"
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        {
          "score": 0,
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          "pathogenic_score": 2,
          "criteria": [
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            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000777597.1",
          "gene_symbol": "ENSG00000301273",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.403-17179C>G",
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      ],
      "clinvar_disease": "Idiopathic generalized epilepsy,Inborn genetic diseases",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:2",
      "phenotype_combined": "Idiopathic generalized epilepsy|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}