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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-81936330-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=81936330&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 81936330,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000564138.6",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.3004C>A",
          "hgvs_p": "p.Leu1002Ile",
          "transcript": "NM_002661.5",
          "protein_id": "NP_002652.2",
          "transcript_support_level": null,
          "aa_start": 1002,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 3004,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": 3185,
          "cdna_end": null,
          "cdna_length": 8666,
          "mane_select": "ENST00000564138.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.3004C>A",
          "hgvs_p": "p.Leu1002Ile",
          "transcript": "ENST00000564138.6",
          "protein_id": "ENSP00000482457.1",
          "transcript_support_level": 1,
          "aa_start": 1002,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 3004,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": 3185,
          "cdna_end": null,
          "cdna_length": 8666,
          "mane_select": "NM_002661.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.3004C>A",
          "hgvs_p": "p.Leu1002Ile",
          "transcript": "NM_001425749.1",
          "protein_id": "NP_001412678.1",
          "transcript_support_level": null,
          "aa_start": 1002,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 3004,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": 3493,
          "cdna_end": null,
          "cdna_length": 4583,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.3004C>A",
          "hgvs_p": "p.Leu1002Ile",
          "transcript": "NM_001425750.1",
          "protein_id": "NP_001412679.1",
          "transcript_support_level": null,
          "aa_start": 1002,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 3004,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": 3396,
          "cdna_end": null,
          "cdna_length": 4486,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.3004C>A",
          "hgvs_p": "p.Leu1002Ile",
          "transcript": "NM_001425751.1",
          "protein_id": "NP_001412680.1",
          "transcript_support_level": null,
          "aa_start": 1002,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 3004,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": 3544,
          "cdna_end": null,
          "cdna_length": 4634,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.3004C>A",
          "hgvs_p": "p.Leu1002Ile",
          "transcript": "ENST00000565054.7",
          "protein_id": "ENSP00000520638.1",
          "transcript_support_level": 5,
          "aa_start": 1002,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 3004,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": 3464,
          "cdna_end": null,
          "cdna_length": 4554,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.3004C>A",
          "hgvs_p": "p.Leu1002Ile",
          "transcript": "ENST00000697580.2",
          "protein_id": "ENSP00000520637.1",
          "transcript_support_level": null,
          "aa_start": 1002,
          "aa_end": null,
          "aa_length": 1265,
          "cds_start": 3004,
          "cds_end": null,
          "cds_length": 3798,
          "cdna_start": 3404,
          "cdna_end": null,
          "cdna_length": 4312,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.2887C>A",
          "hgvs_p": "p.Leu963Ile",
          "transcript": "ENST00000697564.1",
          "protein_id": "ENSP00000513340.1",
          "transcript_support_level": null,
          "aa_start": 963,
          "aa_end": null,
          "aa_length": 1226,
          "cds_start": 2887,
          "cds_end": null,
          "cds_length": 3681,
          "cdna_start": 3046,
          "cdna_end": null,
          "cdna_length": 4119,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.2803C>A",
          "hgvs_p": "p.Leu935Ile",
          "transcript": "ENST00000697583.1",
          "protein_id": "ENSP00000513349.1",
          "transcript_support_level": null,
          "aa_start": 935,
          "aa_end": null,
          "aa_length": 1198,
          "cds_start": 2803,
          "cds_end": null,
          "cds_length": 3597,
          "cdna_start": 3075,
          "cdna_end": null,
          "cdna_length": 4145,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.2803C>A",
          "hgvs_p": "p.Leu935Ile",
          "transcript": "ENST00000697584.1",
          "protein_id": "ENSP00000513350.1",
          "transcript_support_level": null,
          "aa_start": 935,
          "aa_end": null,
          "aa_length": 1198,
          "cds_start": 2803,
          "cds_end": null,
          "cds_length": 3597,
          "cdna_start": 3083,
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          "cdna_length": 4181,
          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "L",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.2803C>A",
          "hgvs_p": "p.Leu935Ile",
          "transcript": "ENST00000697585.1",
          "protein_id": "ENSP00000513351.1",
          "transcript_support_level": null,
          "aa_start": 935,
          "aa_end": null,
          "aa_length": 1198,
          "cds_start": 2803,
          "cds_end": null,
          "cds_length": 3597,
          "cdna_start": 3036,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "L",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
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          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.2803C>A",
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          "transcript": "ENST00000697586.1",
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          "aa_start": 935,
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          "aa_length": 1198,
          "cds_start": 2803,
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          "cdna_start": 2969,
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        },
        {
          "aa_ref": "L",
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          "strand": true,
          "consequences": [
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          ],
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          "exon_rank_end": null,
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "c.2803C>A",
          "hgvs_p": "p.Leu935Ile",
          "transcript": "ENST00000697587.1",
          "protein_id": "ENSP00000513353.1",
          "transcript_support_level": null,
          "aa_start": 935,
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          "cds_start": 2803,
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "n.1179C>A",
          "hgvs_p": null,
          "transcript": "ENST00000563269.2",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
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          "cdna_length": 2196,
          "mane_select": null,
          "mane_plus": null,
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          "feature": null
        },
        {
          "aa_ref": null,
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          "strand": true,
          "consequences": [
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          ],
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          "exon_count": 21,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "n.2122C>A",
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          "transcript": "ENST00000570198.2",
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          "aa_start": null,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "n.*1864C>A",
          "hgvs_p": null,
          "transcript": "ENST00000697562.1",
          "protein_id": "ENSP00000513338.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "cds_start": -4,
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          "cdna_start": null,
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          "cdna_length": 4343,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "n.*2850C>A",
          "hgvs_p": null,
          "transcript": "ENST00000697563.1",
          "protein_id": "ENSP00000513339.1",
          "transcript_support_level": null,
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          "aa_length": null,
          "cds_start": -4,
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          "cds_length": null,
          "cdna_start": null,
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          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "n.*2998C>A",
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "n.*286C>A",
          "hgvs_p": null,
          "transcript": "ENST00000697582.1",
          "protein_id": "ENSP00000513347.1",
          "transcript_support_level": null,
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          "cds_start": -4,
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          "cdna_length": 8742,
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          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "n.*1864C>A",
          "hgvs_p": null,
          "transcript": "ENST00000697562.1",
          "protein_id": "ENSP00000513338.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4343,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 26,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "n.*2850C>A",
          "hgvs_p": null,
          "transcript": "ENST00000697563.1",
          "protein_id": "ENSP00000513339.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4458,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 28,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "n.*2998C>A",
          "hgvs_p": null,
          "transcript": "ENST00000697581.1",
          "protein_id": "ENSP00000513346.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4490,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 27,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLCG2",
          "gene_hgnc_id": 9066,
          "hgvs_c": "n.*286C>A",
          "hgvs_p": null,
          "transcript": "ENST00000697582.1",
          "protein_id": "ENSP00000513347.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 8742,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "PLCG2",
      "gene_hgnc_id": 9066,
      "dbsnp": "rs977656147",
      "frequency_reference_population": 0.000006814977,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 11,
      "gnomad_exomes_af": 0.00000547244,
      "gnomad_genomes_af": 0.0000197081,
      "gnomad_exomes_ac": 8,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.5170303583145142,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.133,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.2893,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.32,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.064,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BS2",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000564138.6",
          "gene_symbol": "PLCG2",
          "hgnc_id": 9066,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.3004C>A",
          "hgvs_p": "p.Leu1002Ile"
        }
      ],
      "clinvar_disease": "Familial cold autoinflammatory syndrome 3,Inborn genetic diseases",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:2",
      "phenotype_combined": "Familial cold autoinflammatory syndrome 3|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}