← Back to variant description

GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-86541774-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=86541774&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 86541774,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001159377.2",
      "consequences": [
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.604G>C",
          "hgvs_p": "p.Asp202His",
          "transcript": "NM_001159377.2",
          "protein_id": "NP_001152849.1",
          "transcript_support_level": null,
          "aa_start": 202,
          "aa_end": null,
          "aa_length": 383,
          "cds_start": 604,
          "cds_end": null,
          "cds_length": 1152,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000360900.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001159377.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.604G>C",
          "hgvs_p": "p.Asp202His",
          "transcript": "ENST00000360900.11",
          "protein_id": "ENSP00000354152.6",
          "transcript_support_level": 1,
          "aa_start": 202,
          "aa_end": null,
          "aa_length": 383,
          "cds_start": 604,
          "cds_end": null,
          "cds_length": 1152,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001159377.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000360900.11"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.604G>C",
          "hgvs_p": "p.Asp202His",
          "transcript": "ENST00000381214.9",
          "protein_id": "ENSP00000370612.5",
          "transcript_support_level": 1,
          "aa_start": 202,
          "aa_end": null,
          "aa_length": 383,
          "cds_start": 604,
          "cds_end": null,
          "cds_length": 1152,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000381214.9"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.601G>C",
          "hgvs_p": "p.Asp201His",
          "transcript": "ENST00000543303.6",
          "protein_id": "ENSP00000444003.2",
          "transcript_support_level": 1,
          "aa_start": 201,
          "aa_end": null,
          "aa_length": 382,
          "cds_start": 601,
          "cds_end": null,
          "cds_length": 1149,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000543303.6"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.601G>C",
          "hgvs_p": "p.Asp201His",
          "transcript": "ENST00000634347.1",
          "protein_id": "ENSP00000489295.1",
          "transcript_support_level": 1,
          "aa_start": 201,
          "aa_end": null,
          "aa_length": 382,
          "cds_start": 601,
          "cds_end": null,
          "cds_length": 1149,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000634347.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.586G>C",
          "hgvs_p": "p.Asp196His",
          "transcript": "ENST00000562940.6",
          "protein_id": "ENSP00000456652.2",
          "transcript_support_level": 1,
          "aa_start": 196,
          "aa_end": null,
          "aa_length": 223,
          "cds_start": 586,
          "cds_end": null,
          "cds_length": 672,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000562940.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "n.*409G>C",
          "hgvs_p": null,
          "transcript": "ENST00000566050.5",
          "protein_id": "ENSP00000456910.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000566050.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "n.*409G>C",
          "hgvs_p": null,
          "transcript": "ENST00000566050.5",
          "protein_id": "ENSP00000456910.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000566050.5"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.604G>C",
          "hgvs_p": "p.Asp202His",
          "transcript": "NM_001159378.2",
          "protein_id": "NP_001152850.1",
          "transcript_support_level": null,
          "aa_start": 202,
          "aa_end": null,
          "aa_length": 383,
          "cds_start": 604,
          "cds_end": null,
          "cds_length": 1152,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001159378.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.601G>C",
          "hgvs_p": "p.Asp201His",
          "transcript": "NM_001159379.2",
          "protein_id": "NP_001152851.1",
          "transcript_support_level": null,
          "aa_start": 201,
          "aa_end": null,
          "aa_length": 382,
          "cds_start": 601,
          "cds_end": null,
          "cds_length": 1149,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001159379.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.601G>C",
          "hgvs_p": "p.Asp201His",
          "transcript": "NM_022764.3",
          "protein_id": "NP_073601.2",
          "transcript_support_level": null,
          "aa_start": 201,
          "aa_end": null,
          "aa_length": 382,
          "cds_start": 601,
          "cds_end": null,
          "cds_length": 1149,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_022764.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.544G>C",
          "hgvs_p": "p.Asp182His",
          "transcript": "NM_001159380.2",
          "protein_id": "NP_001152852.1",
          "transcript_support_level": null,
          "aa_start": 182,
          "aa_end": null,
          "aa_length": 363,
          "cds_start": 544,
          "cds_end": null,
          "cds_length": 1092,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001159380.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.169G>C",
          "hgvs_p": "p.Asp57His",
          "transcript": "ENST00000950129.1",
          "protein_id": "ENSP00000620188.1",
          "transcript_support_level": null,
          "aa_start": 57,
          "aa_end": null,
          "aa_length": 238,
          "cds_start": 169,
          "cds_end": null,
          "cds_length": 717,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000950129.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.583G>C",
          "hgvs_p": "p.Asp195His",
          "transcript": "ENST00000566469.5",
          "protein_id": "ENSP00000455940.1",
          "transcript_support_level": 2,
          "aa_start": 195,
          "aa_end": null,
          "aa_length": 232,
          "cds_start": 583,
          "cds_end": null,
          "cds_length": 699,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000566469.5"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.115G>C",
          "hgvs_p": "p.Asp39His",
          "transcript": "ENST00000546093.5",
          "protein_id": "ENSP00000438761.1",
          "transcript_support_level": 2,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 220,
          "cds_start": 115,
          "cds_end": null,
          "cds_length": 663,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000546093.5"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.487G>C",
          "hgvs_p": "p.Asp163His",
          "transcript": "ENST00000569000.5",
          "protein_id": "ENSP00000454807.1",
          "transcript_support_level": 4,
          "aa_start": 163,
          "aa_end": null,
          "aa_length": 173,
          "cds_start": 487,
          "cds_end": null,
          "cds_length": 522,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000569000.5"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.544G>C",
          "hgvs_p": "p.Asp182His",
          "transcript": "XM_005256101.2",
          "protein_id": "XP_005256158.1",
          "transcript_support_level": null,
          "aa_start": 182,
          "aa_end": null,
          "aa_length": 363,
          "cds_start": 544,
          "cds_end": null,
          "cds_length": 1092,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005256101.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.544G>C",
          "hgvs_p": "p.Asp182His",
          "transcript": "XM_011523280.3",
          "protein_id": "XP_011521582.1",
          "transcript_support_level": null,
          "aa_start": 182,
          "aa_end": null,
          "aa_length": 363,
          "cds_start": 544,
          "cds_end": null,
          "cds_length": 1092,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011523280.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.544G>C",
          "hgvs_p": "p.Asp182His",
          "transcript": "XM_047434497.1",
          "protein_id": "XP_047290453.1",
          "transcript_support_level": null,
          "aa_start": 182,
          "aa_end": null,
          "aa_length": 363,
          "cds_start": 544,
          "cds_end": null,
          "cds_length": 1092,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434497.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.544G>C",
          "hgvs_p": "p.Asp182His",
          "transcript": "XM_047434498.1",
          "protein_id": "XP_047290454.1",
          "transcript_support_level": null,
          "aa_start": 182,
          "aa_end": null,
          "aa_length": 363,
          "cds_start": 544,
          "cds_end": null,
          "cds_length": 1092,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434498.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.544G>C",
          "hgvs_p": "p.Asp182His",
          "transcript": "XM_047434499.1",
          "protein_id": "XP_047290455.1",
          "transcript_support_level": null,
          "aa_start": 182,
          "aa_end": null,
          "aa_length": 363,
          "cds_start": 544,
          "cds_end": null,
          "cds_length": 1092,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434499.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.490G>C",
          "hgvs_p": "p.Asp164His",
          "transcript": "XM_017023571.2",
          "protein_id": "XP_016879060.1",
          "transcript_support_level": null,
          "aa_start": 164,
          "aa_end": null,
          "aa_length": 345,
          "cds_start": 490,
          "cds_end": null,
          "cds_length": 1038,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023571.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.487G>C",
          "hgvs_p": "p.Asp163His",
          "transcript": "XM_017023572.3",
          "protein_id": "XP_016879061.3",
          "transcript_support_level": null,
          "aa_start": 163,
          "aa_end": null,
          "aa_length": 344,
          "cds_start": 487,
          "cds_end": null,
          "cds_length": 1035,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017023572.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.229G>C",
          "hgvs_p": "p.Asp77His",
          "transcript": "XM_047434503.1",
          "protein_id": "XP_047290459.1",
          "transcript_support_level": null,
          "aa_start": 77,
          "aa_end": null,
          "aa_length": 258,
          "cds_start": 229,
          "cds_end": null,
          "cds_length": 777,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434503.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.178G>C",
          "hgvs_p": "p.Asp60His",
          "transcript": "XM_047434504.1",
          "protein_id": "XP_047290460.1",
          "transcript_support_level": null,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 241,
          "cds_start": 178,
          "cds_end": null,
          "cds_length": 726,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434504.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.604G>C",
          "hgvs_p": "p.Asp202His",
          "transcript": "XM_011523282.3",
          "protein_id": "XP_011521584.1",
          "transcript_support_level": null,
          "aa_start": 202,
          "aa_end": null,
          "aa_length": 231,
          "cds_start": 604,
          "cds_end": null,
          "cds_length": 696,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011523282.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.604G>C",
          "hgvs_p": "p.Asp202His",
          "transcript": "XM_047434500.1",
          "protein_id": "XP_047290456.1",
          "transcript_support_level": null,
          "aa_start": 202,
          "aa_end": null,
          "aa_length": 231,
          "cds_start": 604,
          "cds_end": null,
          "cds_length": 696,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434500.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.604G>C",
          "hgvs_p": "p.Asp202His",
          "transcript": "XM_011523283.3",
          "protein_id": "XP_011521585.1",
          "transcript_support_level": null,
          "aa_start": 202,
          "aa_end": null,
          "aa_length": 229,
          "cds_start": 604,
          "cds_end": null,
          "cds_length": 690,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011523283.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.604G>C",
          "hgvs_p": "p.Asp202His",
          "transcript": "XM_011523284.3",
          "protein_id": "XP_011521586.1",
          "transcript_support_level": null,
          "aa_start": 202,
          "aa_end": null,
          "aa_length": 229,
          "cds_start": 604,
          "cds_end": null,
          "cds_length": 690,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011523284.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.601G>C",
          "hgvs_p": "p.Asp201His",
          "transcript": "XM_047434501.1",
          "protein_id": "XP_047290457.1",
          "transcript_support_level": null,
          "aa_start": 201,
          "aa_end": null,
          "aa_length": 228,
          "cds_start": 601,
          "cds_end": null,
          "cds_length": 687,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434501.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.601G>C",
          "hgvs_p": "p.Asp201His",
          "transcript": "XM_047434502.1",
          "protein_id": "XP_047290458.1",
          "transcript_support_level": null,
          "aa_start": 201,
          "aa_end": null,
          "aa_length": 228,
          "cds_start": 601,
          "cds_end": null,
          "cds_length": 687,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434502.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.115G>C",
          "hgvs_p": "p.Asp39His",
          "transcript": "XM_047434506.1",
          "protein_id": "XP_047290462.1",
          "transcript_support_level": null,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 220,
          "cds_start": 115,
          "cds_end": null,
          "cds_length": 663,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434506.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.115G>C",
          "hgvs_p": "p.Asp39His",
          "transcript": "XM_047434507.1",
          "protein_id": "XP_047290463.1",
          "transcript_support_level": null,
          "aa_start": 39,
          "aa_end": null,
          "aa_length": 220,
          "cds_start": 115,
          "cds_end": null,
          "cds_length": 663,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434507.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.544G>C",
          "hgvs_p": "p.Asp182His",
          "transcript": "XM_047434508.1",
          "protein_id": "XP_047290464.1",
          "transcript_support_level": null,
          "aa_start": 182,
          "aa_end": null,
          "aa_length": 209,
          "cds_start": 544,
          "cds_end": null,
          "cds_length": 630,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434508.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "c.490G>C",
          "hgvs_p": "p.Asp164His",
          "transcript": "XM_047434505.1",
          "protein_id": "XP_047290461.1",
          "transcript_support_level": null,
          "aa_start": 164,
          "aa_end": null,
          "aa_length": 191,
          "cds_start": 490,
          "cds_end": null,
          "cds_length": 576,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047434505.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "n.494G>C",
          "hgvs_p": null,
          "transcript": "ENST00000562096.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000562096.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "n.*111G>C",
          "hgvs_p": null,
          "transcript": "ENST00000567539.5",
          "protein_id": "ENSP00000454593.1",
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000567539.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "n.725G>C",
          "hgvs_p": null,
          "transcript": "NR_027489.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_027489.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "n.715G>C",
          "hgvs_p": null,
          "transcript": "NR_027490.2",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_027490.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MTHFSD",
          "gene_hgnc_id": 25778,
          "hgvs_c": "n.*111G>C",
          "hgvs_p": null,
          "transcript": "ENST00000567539.5",
          "protein_id": "ENSP00000454593.1",
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000567539.5"
        }
      ],
      "gene_symbol": "MTHFSD",
      "gene_hgnc_id": 25778,
      "dbsnp": "rs201325688",
      "frequency_reference_population": 0.00038161082,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 616,
      "gnomad_exomes_af": 0.000396071,
      "gnomad_genomes_af": 0.000242859,
      "gnomad_exomes_ac": 579,
      "gnomad_genomes_ac": 37,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8303865790367126,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.684,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.7652,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.25,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.201,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PP3",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 0,
          "pathogenic_score": 1,
          "criteria": [
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001159377.2",
          "gene_symbol": "MTHFSD",
          "hgnc_id": 25778,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.604G>C",
          "hgvs_p": "p.Asp202His"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}