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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 16-88697611-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=16&pos=88697611&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "16",
      "pos": 88697611,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_178841.4",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "c.671C>T",
          "hgvs_p": "p.Ala224Val",
          "transcript": "NM_178841.4",
          "protein_id": "NP_849163.1",
          "transcript_support_level": null,
          "aa_start": 224,
          "aa_end": null,
          "aa_length": 237,
          "cds_start": 671,
          "cds_end": null,
          "cds_length": 714,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000312838.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_178841.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "c.671C>T",
          "hgvs_p": "p.Ala224Val",
          "transcript": "ENST00000312838.9",
          "protein_id": "ENSP00000326095.4",
          "transcript_support_level": 1,
          "aa_start": 224,
          "aa_end": null,
          "aa_length": 237,
          "cds_start": 671,
          "cds_end": null,
          "cds_length": 714,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_178841.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000312838.9"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "c.668C>T",
          "hgvs_p": "p.Ala223Val",
          "transcript": "ENST00000956472.1",
          "protein_id": "ENSP00000626531.1",
          "transcript_support_level": null,
          "aa_start": 223,
          "aa_end": null,
          "aa_length": 236,
          "cds_start": 668,
          "cds_end": null,
          "cds_length": 711,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956472.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "c.656C>T",
          "hgvs_p": "p.Ala219Val",
          "transcript": "ENST00000878562.1",
          "protein_id": "ENSP00000548621.1",
          "transcript_support_level": null,
          "aa_start": 219,
          "aa_end": null,
          "aa_length": 232,
          "cds_start": 656,
          "cds_end": null,
          "cds_length": 699,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000878562.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "c.428C>T",
          "hgvs_p": "p.Ala143Val",
          "transcript": "NM_001171815.2",
          "protein_id": "NP_001165286.1",
          "transcript_support_level": null,
          "aa_start": 143,
          "aa_end": null,
          "aa_length": 156,
          "cds_start": 428,
          "cds_end": null,
          "cds_length": 471,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001171815.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "c.428C>T",
          "hgvs_p": "p.Ala143Val",
          "transcript": "ENST00000567844.1",
          "protein_id": "ENSP00000457336.1",
          "transcript_support_level": 5,
          "aa_start": 143,
          "aa_end": null,
          "aa_length": 156,
          "cds_start": 428,
          "cds_end": null,
          "cds_length": 471,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000567844.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "c.344C>T",
          "hgvs_p": "p.Ala115Val",
          "transcript": "NM_001171816.2",
          "protein_id": "NP_001165287.1",
          "transcript_support_level": null,
          "aa_start": 115,
          "aa_end": null,
          "aa_length": 128,
          "cds_start": 344,
          "cds_end": null,
          "cds_length": 387,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001171816.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "c.344C>T",
          "hgvs_p": "p.Ala115Val",
          "transcript": "ENST00000537718.6",
          "protein_id": "ENSP00000446301.2",
          "transcript_support_level": 2,
          "aa_start": 115,
          "aa_end": null,
          "aa_length": 128,
          "cds_start": 344,
          "cds_end": null,
          "cds_length": 387,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000537718.6"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "c.344C>T",
          "hgvs_p": "p.Ala115Val",
          "transcript": "ENST00000541206.6",
          "protein_id": "ENSP00000440454.2",
          "transcript_support_level": 2,
          "aa_start": 115,
          "aa_end": null,
          "aa_length": 128,
          "cds_start": 344,
          "cds_end": null,
          "cds_length": 387,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000541206.6"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "c.1010C>T",
          "hgvs_p": "p.Ala337Val",
          "transcript": "XM_011522845.3",
          "protein_id": "XP_011521147.1",
          "transcript_support_level": null,
          "aa_start": 337,
          "aa_end": null,
          "aa_length": 350,
          "cds_start": 1010,
          "cds_end": null,
          "cds_length": 1053,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011522845.3"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "c.344C>T",
          "hgvs_p": "p.Ala115Val",
          "transcript": "XM_047433580.1",
          "protein_id": "XP_047289536.1",
          "transcript_support_level": null,
          "aa_start": 115,
          "aa_end": null,
          "aa_length": 128,
          "cds_start": 344,
          "cds_end": null,
          "cds_length": 387,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047433580.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "c.*714C>T",
          "hgvs_p": null,
          "transcript": "ENST00000568683.5",
          "protein_id": "ENSP00000457374.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 165,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 498,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000568683.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "c.*9C>T",
          "hgvs_p": null,
          "transcript": "XM_047433578.1",
          "protein_id": "XP_047289534.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 272,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 819,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047433578.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "c.363-973C>T",
          "hgvs_p": null,
          "transcript": "ENST00000562544.1",
          "protein_id": "ENSP00000455539.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 148,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 447,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000562544.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "c.102-902C>T",
          "hgvs_p": null,
          "transcript": "ENST00000567408.1",
          "protein_id": "ENSP00000455617.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 45,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 138,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000567408.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RNF166",
          "gene_hgnc_id": 28856,
          "hgvs_c": "n.273C>T",
          "hgvs_p": null,
          "transcript": "ENST00000565083.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000565083.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000259813",
          "gene_hgnc_id": null,
          "hgvs_c": "n.144-930G>A",
          "hgvs_p": null,
          "transcript": "ENST00000561699.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000561699.1"
        }
      ],
      "gene_symbol": "RNF166",
      "gene_hgnc_id": 28856,
      "dbsnp": "rs367841158",
      "frequency_reference_population": 0.000025129093,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 39,
      "gnomad_exomes_af": 0.0000271472,
      "gnomad_genomes_af": 0.00000656978,
      "gnomad_exomes_ac": 38,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.2978978157043457,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.11999999731779099,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.262,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.4905,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.39,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 7.444,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.12,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_178841.4",
          "gene_symbol": "RNF166",
          "hgnc_id": 28856,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.671C>T",
          "hgvs_p": "p.Ala224Val"
        },
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000561699.1",
          "gene_symbol": "ENSG00000259813",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.144-930G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}