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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-17346827-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=17346827&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "17",
      "pos": 17346827,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001438936.1",
      "consequences": [
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "c.567G>C",
          "hgvs_p": "p.Trp189Cys",
          "transcript": "NM_020201.4",
          "protein_id": "NP_064586.1",
          "transcript_support_level": null,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 228,
          "cds_start": 567,
          "cds_end": null,
          "cds_length": 687,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000389022.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_020201.4"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "c.567G>C",
          "hgvs_p": "p.Trp189Cys",
          "transcript": "ENST00000389022.9",
          "protein_id": "ENSP00000373674.4",
          "transcript_support_level": 1,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 228,
          "cds_start": 567,
          "cds_end": null,
          "cds_length": 687,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_020201.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000389022.9"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "c.585G>C",
          "hgvs_p": "p.Trp195Cys",
          "transcript": "ENST00000616989.1",
          "protein_id": "ENSP00000481269.1",
          "transcript_support_level": 1,
          "aa_start": 195,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 585,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000616989.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "c.678G>C",
          "hgvs_p": "p.Trp226Cys",
          "transcript": "NM_001438936.1",
          "protein_id": "NP_001425865.1",
          "transcript_support_level": null,
          "aa_start": 226,
          "aa_end": null,
          "aa_length": 265,
          "cds_start": 678,
          "cds_end": null,
          "cds_length": 798,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001438936.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "c.660G>C",
          "hgvs_p": "p.Trp220Cys",
          "transcript": "NM_001438937.1",
          "protein_id": "NP_001425866.1",
          "transcript_support_level": null,
          "aa_start": 220,
          "aa_end": null,
          "aa_length": 259,
          "cds_start": 660,
          "cds_end": null,
          "cds_length": 780,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001438937.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "c.660G>C",
          "hgvs_p": "p.Trp220Cys",
          "transcript": "ENST00000879604.1",
          "protein_id": "ENSP00000549663.1",
          "transcript_support_level": null,
          "aa_start": 220,
          "aa_end": null,
          "aa_length": 259,
          "cds_start": 660,
          "cds_end": null,
          "cds_length": 780,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000879604.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "c.585G>C",
          "hgvs_p": "p.Trp195Cys",
          "transcript": "NM_001438938.1",
          "protein_id": "NP_001425867.1",
          "transcript_support_level": null,
          "aa_start": 195,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 585,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001438938.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "c.735G>C",
          "hgvs_p": "p.Trp245Cys",
          "transcript": "XM_047436419.1",
          "protein_id": "XP_047292375.1",
          "transcript_support_level": null,
          "aa_start": 245,
          "aa_end": null,
          "aa_length": 284,
          "cds_start": 735,
          "cds_end": null,
          "cds_length": 855,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047436419.1"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "c.642G>C",
          "hgvs_p": "p.Trp214Cys",
          "transcript": "XM_005256731.4",
          "protein_id": "XP_005256788.1",
          "transcript_support_level": null,
          "aa_start": 214,
          "aa_end": null,
          "aa_length": 253,
          "cds_start": 642,
          "cds_end": null,
          "cds_length": 762,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005256731.4"
        },
        {
          "aa_ref": "W",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "c.327G>C",
          "hgvs_p": "p.Trp109Cys",
          "transcript": "XM_011523964.2",
          "protein_id": "XP_011522266.1",
          "transcript_support_level": null,
          "aa_start": 109,
          "aa_end": null,
          "aa_length": 148,
          "cds_start": 327,
          "cds_end": null,
          "cds_length": 447,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011523964.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "c.*67G>C",
          "hgvs_p": null,
          "transcript": "XM_047436421.1",
          "protein_id": "XP_047292377.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 231,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 696,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047436421.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "c.*67G>C",
          "hgvs_p": null,
          "transcript": "XM_047436422.1",
          "protein_id": "XP_047292378.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 225,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 678,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047436422.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "n.*421G>C",
          "hgvs_p": null,
          "transcript": "ENST00000470418.5",
          "protein_id": "ENSP00000463674.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000470418.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "n.67G>C",
          "hgvs_p": null,
          "transcript": "ENST00000480529.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000480529.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "n.*417G>C",
          "hgvs_p": null,
          "transcript": "ENST00000483704.5",
          "protein_id": "ENSP00000467567.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000483704.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "n.234G>C",
          "hgvs_p": null,
          "transcript": "ENST00000582909.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000582909.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "n.*421G>C",
          "hgvs_p": null,
          "transcript": "ENST00000470418.5",
          "protein_id": "ENSP00000463674.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000470418.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NT5M",
          "gene_hgnc_id": 15769,
          "hgvs_c": "n.*417G>C",
          "hgvs_p": null,
          "transcript": "ENST00000483704.5",
          "protein_id": "ENSP00000467567.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000483704.5"
        }
      ],
      "gene_symbol": "NT5M",
      "gene_hgnc_id": 15769,
      "dbsnp": "rs146354908",
      "frequency_reference_population": 0.000014926635,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 24,
      "gnomad_exomes_af": 0.0000137392,
      "gnomad_genomes_af": 0.0000262854,
      "gnomad_exomes_ac": 20,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.943243145942688,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.783,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.9972,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.21,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 8.647,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 6,
      "acmg_classification": "Likely_pathogenic",
      "acmg_criteria": "PM2,PP3_Strong",
      "acmg_by_gene": [
        {
          "score": 6,
          "benign_score": 0,
          "pathogenic_score": 6,
          "criteria": [
            "PM2",
            "PP3_Strong"
          ],
          "verdict": "Likely_pathogenic",
          "transcript": "NM_001438936.1",
          "gene_symbol": "NT5M",
          "hgnc_id": 15769,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.678G>C",
          "hgvs_p": "p.Trp226Cys"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}