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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-40184257-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=40184257&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "17",
      "pos": 40184257,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_016339.6",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAPGEFL1",
          "gene_hgnc_id": 17428,
          "hgvs_c": "c.643C>T",
          "hgvs_p": "p.Arg215Trp",
          "transcript": "NM_016339.6",
          "protein_id": "NP_057423.2",
          "transcript_support_level": null,
          "aa_start": 215,
          "aa_end": null,
          "aa_length": 662,
          "cds_start": 643,
          "cds_end": null,
          "cds_length": 1989,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000620260.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_016339.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAPGEFL1",
          "gene_hgnc_id": 17428,
          "hgvs_c": "c.643C>T",
          "hgvs_p": "p.Arg215Trp",
          "transcript": "ENST00000620260.6",
          "protein_id": "ENSP00000479735.1",
          "transcript_support_level": 1,
          "aa_start": 215,
          "aa_end": null,
          "aa_length": 662,
          "cds_start": 643,
          "cds_end": null,
          "cds_length": 1989,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_016339.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000620260.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAPGEFL1",
          "gene_hgnc_id": 17428,
          "hgvs_c": "c.190C>T",
          "hgvs_p": "p.Arg64Trp",
          "transcript": "ENST00000456989.6",
          "protein_id": "ENSP00000394530.2",
          "transcript_support_level": 1,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 511,
          "cds_start": 190,
          "cds_end": null,
          "cds_length": 1536,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000456989.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAPGEFL1",
          "gene_hgnc_id": 17428,
          "hgvs_c": "c.172C>T",
          "hgvs_p": "p.Arg58Trp",
          "transcript": "ENST00000544503.5",
          "protein_id": "ENSP00000438631.1",
          "transcript_support_level": 2,
          "aa_start": 58,
          "aa_end": null,
          "aa_length": 505,
          "cds_start": 172,
          "cds_end": null,
          "cds_length": 1518,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000544503.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAPGEFL1",
          "gene_hgnc_id": 17428,
          "hgvs_c": "c.25C>T",
          "hgvs_p": "p.Arg9Trp",
          "transcript": "ENST00000264644.10",
          "protein_id": "ENSP00000264644.5",
          "transcript_support_level": 5,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 456,
          "cds_start": 25,
          "cds_end": null,
          "cds_length": 1371,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000264644.10"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAPGEFL1",
          "gene_hgnc_id": 17428,
          "hgvs_c": "c.25C>T",
          "hgvs_p": "p.Arg9Trp",
          "transcript": "ENST00000543876.5",
          "protein_id": "ENSP00000440226.1",
          "transcript_support_level": 4,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 148,
          "cds_start": 25,
          "cds_end": null,
          "cds_length": 447,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000543876.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAPGEFL1",
          "gene_hgnc_id": 17428,
          "hgvs_c": "c.25C>T",
          "hgvs_p": "p.Arg9Trp",
          "transcript": "ENST00000538981.1",
          "protein_id": "ENSP00000441059.1",
          "transcript_support_level": 2,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 100,
          "cds_start": 25,
          "cds_end": null,
          "cds_length": 304,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000538981.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAPGEFL1",
          "gene_hgnc_id": 17428,
          "hgvs_c": "c.136C>T",
          "hgvs_p": "p.Arg46Trp",
          "transcript": "ENST00000541245.1",
          "protein_id": "ENSP00000444646.1",
          "transcript_support_level": 3,
          "aa_start": 46,
          "aa_end": null,
          "aa_length": 61,
          "cds_start": 136,
          "cds_end": null,
          "cds_length": 187,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000541245.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAPGEFL1",
          "gene_hgnc_id": 17428,
          "hgvs_c": "c.25C>T",
          "hgvs_p": "p.Arg9Trp",
          "transcript": "ENST00000538884.1",
          "protein_id": "ENSP00000440006.1",
          "transcript_support_level": 4,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 19,
          "cds_start": 25,
          "cds_end": null,
          "cds_length": 62,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000538884.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAPGEFL1",
          "gene_hgnc_id": 17428,
          "hgvs_c": "c.190C>T",
          "hgvs_p": "p.Arg64Trp",
          "transcript": "NM_001303533.2",
          "protein_id": "NP_001290462.1",
          "transcript_support_level": null,
          "aa_start": 64,
          "aa_end": null,
          "aa_length": 511,
          "cds_start": 190,
          "cds_end": null,
          "cds_length": 1536,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001303533.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAPGEFL1",
          "gene_hgnc_id": 17428,
          "hgvs_c": "c.172C>T",
          "hgvs_p": "p.Arg58Trp",
          "transcript": "NM_001303534.3",
          "protein_id": "NP_001290463.1",
          "transcript_support_level": null,
          "aa_start": 58,
          "aa_end": null,
          "aa_length": 505,
          "cds_start": 172,
          "cds_end": null,
          "cds_length": 1518,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001303534.3"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAPGEFL1",
          "gene_hgnc_id": 17428,
          "hgvs_c": "c.643C>T",
          "hgvs_p": "p.Arg215Trp",
          "transcript": "XM_047436203.1",
          "protein_id": "XP_047292159.1",
          "transcript_support_level": null,
          "aa_start": 215,
          "aa_end": null,
          "aa_length": 662,
          "cds_start": 643,
          "cds_end": null,
          "cds_length": 1989,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047436203.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAPGEFL1",
          "gene_hgnc_id": 17428,
          "hgvs_c": "c.136C>T",
          "hgvs_p": "p.Arg46Trp",
          "transcript": "XM_047436204.1",
          "protein_id": "XP_047292160.1",
          "transcript_support_level": null,
          "aa_start": 46,
          "aa_end": null,
          "aa_length": 493,
          "cds_start": 136,
          "cds_end": null,
          "cds_length": 1482,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047436204.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RAPGEFL1",
          "gene_hgnc_id": 17428,
          "hgvs_c": "c.25C>T",
          "hgvs_p": "p.Arg9Trp",
          "transcript": "XM_047436205.1",
          "protein_id": "XP_047292161.1",
          "transcript_support_level": null,
          "aa_start": 9,
          "aa_end": null,
          "aa_length": 456,
          "cds_start": 25,
          "cds_end": null,
          "cds_length": 1371,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047436205.1"
        }
      ],
      "gene_symbol": "RAPGEFL1",
      "gene_hgnc_id": 17428,
      "dbsnp": "rs765143011",
      "frequency_reference_population": 0.00003284931,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 53,
      "gnomad_exomes_af": 0.0000328458,
      "gnomad_genomes_af": 0.0000328826,
      "gnomad_exomes_ac": 48,
      "gnomad_genomes_ac": 5,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.26755595207214355,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.221,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1824,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.32,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.559,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_016339.6",
          "gene_symbol": "RAPGEFL1",
          "hgnc_id": 17428,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.643C>T",
          "hgvs_p": "p.Arg215Trp"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}