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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-43529634-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=43529634&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "17",
      "pos": 43529634,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001986.4",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.998C>T",
          "hgvs_p": "p.Pro333Leu",
          "transcript": "NM_001079675.5",
          "protein_id": "NP_001073143.1",
          "transcript_support_level": null,
          "aa_start": 333,
          "aa_end": null,
          "aa_length": 484,
          "cds_start": 998,
          "cds_end": null,
          "cds_length": 1455,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000319349.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001079675.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.998C>T",
          "hgvs_p": "p.Pro333Leu",
          "transcript": "ENST00000319349.10",
          "protein_id": "ENSP00000321835.4",
          "transcript_support_level": 1,
          "aa_start": 333,
          "aa_end": null,
          "aa_length": 484,
          "cds_start": 998,
          "cds_end": null,
          "cds_length": 1455,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001079675.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000319349.10"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.998C>T",
          "hgvs_p": "p.Pro333Leu",
          "transcript": "ENST00000393664.6",
          "protein_id": "ENSP00000377273.1",
          "transcript_support_level": 1,
          "aa_start": 333,
          "aa_end": null,
          "aa_length": 484,
          "cds_start": 998,
          "cds_end": null,
          "cds_length": 1455,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000393664.6"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.998C>T",
          "hgvs_p": "p.Pro333Leu",
          "transcript": "ENST00000591713.5",
          "protein_id": "ENSP00000465718.1",
          "transcript_support_level": 1,
          "aa_start": 333,
          "aa_end": null,
          "aa_length": 484,
          "cds_start": 998,
          "cds_end": null,
          "cds_length": 1455,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000591713.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.167C>T",
          "hgvs_p": "p.Pro56Leu",
          "transcript": "ENST00000586826.1",
          "protein_id": "ENSP00000468636.1",
          "transcript_support_level": 1,
          "aa_start": 56,
          "aa_end": null,
          "aa_length": 207,
          "cds_start": 167,
          "cds_end": null,
          "cds_length": 624,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000586826.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.998C>T",
          "hgvs_p": "p.Pro333Leu",
          "transcript": "NM_001369366.2",
          "protein_id": "NP_001356295.1",
          "transcript_support_level": null,
          "aa_start": 333,
          "aa_end": null,
          "aa_length": 484,
          "cds_start": 998,
          "cds_end": null,
          "cds_length": 1455,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001369366.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.998C>T",
          "hgvs_p": "p.Pro333Leu",
          "transcript": "NM_001986.4",
          "protein_id": "NP_001977.1",
          "transcript_support_level": null,
          "aa_start": 333,
          "aa_end": null,
          "aa_length": 484,
          "cds_start": 998,
          "cds_end": null,
          "cds_length": 1455,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001986.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.998C>T",
          "hgvs_p": "p.Pro333Leu",
          "transcript": "ENST00000857844.1",
          "protein_id": "ENSP00000527903.1",
          "transcript_support_level": null,
          "aa_start": 333,
          "aa_end": null,
          "aa_length": 484,
          "cds_start": 998,
          "cds_end": null,
          "cds_length": 1455,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857844.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.998C>T",
          "hgvs_p": "p.Pro333Leu",
          "transcript": "ENST00000922775.1",
          "protein_id": "ENSP00000592834.1",
          "transcript_support_level": null,
          "aa_start": 333,
          "aa_end": null,
          "aa_length": 484,
          "cds_start": 998,
          "cds_end": null,
          "cds_length": 1455,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922775.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.995C>T",
          "hgvs_p": "p.Pro332Leu",
          "transcript": "NM_001369367.2",
          "protein_id": "NP_001356296.1",
          "transcript_support_level": null,
          "aa_start": 332,
          "aa_end": null,
          "aa_length": 483,
          "cds_start": 995,
          "cds_end": null,
          "cds_length": 1452,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001369367.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.983C>T",
          "hgvs_p": "p.Pro328Leu",
          "transcript": "NM_001369368.2",
          "protein_id": "NP_001356297.1",
          "transcript_support_level": null,
          "aa_start": 328,
          "aa_end": null,
          "aa_length": 479,
          "cds_start": 983,
          "cds_end": null,
          "cds_length": 1440,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001369368.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.983C>T",
          "hgvs_p": "p.Pro328Leu",
          "transcript": "ENST00000857843.1",
          "protein_id": "ENSP00000527902.1",
          "transcript_support_level": null,
          "aa_start": 328,
          "aa_end": null,
          "aa_length": 479,
          "cds_start": 983,
          "cds_end": null,
          "cds_length": 1440,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857843.1"
        },
        {
          "aa_ref": "P",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.983C>T",
          "hgvs_p": "p.Pro328Leu",
          "transcript": "ENST00000922774.1",
          "protein_id": "ENSP00000592833.1",
          "transcript_support_level": null,
          "aa_start": 328,
          "aa_end": null,
          "aa_length": 479,
          "cds_start": 983,
          "cds_end": null,
          "cds_length": 1440,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922774.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.938C>T",
          "hgvs_p": "p.Pro313Leu",
          "transcript": "ENST00000922776.1",
          "protein_id": "ENSP00000592835.1",
          "transcript_support_level": null,
          "aa_start": 313,
          "aa_end": null,
          "aa_length": 464,
          "cds_start": 938,
          "cds_end": null,
          "cds_length": 1395,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000922776.1"
        },
        {
          "aa_ref": "P",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.923C>T",
          "hgvs_p": "p.Pro308Leu",
          "transcript": "ENST00000922777.1",
          "protein_id": "ENSP00000592836.1",
          "transcript_support_level": null,
          "aa_start": 308,
          "aa_end": null,
          "aa_length": 459,
          "cds_start": 923,
          "cds_end": null,
          "cds_length": 1380,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922777.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.881C>T",
          "hgvs_p": "p.Pro294Leu",
          "transcript": "NM_001261437.3",
          "protein_id": "NP_001248366.1",
          "transcript_support_level": null,
          "aa_start": 294,
          "aa_end": null,
          "aa_length": 445,
          "cds_start": 881,
          "cds_end": null,
          "cds_length": 1338,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001261437.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.881C>T",
          "hgvs_p": "p.Pro294Leu",
          "transcript": "NM_001261438.3",
          "protein_id": "NP_001248367.1",
          "transcript_support_level": null,
          "aa_start": 294,
          "aa_end": null,
          "aa_length": 445,
          "cds_start": 881,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001261438.3"
        },
        {
          "aa_ref": "P",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.881C>T",
          "hgvs_p": "p.Pro294Leu",
          "transcript": "ENST00000538265.5",
          "protein_id": "ENSP00000443846.1",
          "transcript_support_level": 2,
          "aa_start": 294,
          "aa_end": null,
          "aa_length": 445,
          "cds_start": 881,
          "cds_end": null,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "P",
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          "consequences": [
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          ],
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          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.881C>T",
          "hgvs_p": "p.Pro294Leu",
          "transcript": "ENST00000545954.5",
          "protein_id": "ENSP00000440023.1",
          "transcript_support_level": 2,
          "aa_start": 294,
          "aa_end": null,
          "aa_length": 445,
          "cds_start": 881,
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          "cds_length": 1338,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000545954.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETV4",
          "gene_hgnc_id": 3493,
          "hgvs_c": "c.836C>T",
          "hgvs_p": "p.Pro279Leu",
          "transcript": "ENST00000545089.5",
          "protein_id": "ENSP00000441749.1",
          "transcript_support_level": 2,
          "aa_start": 279,
          "aa_end": null,
          "aa_length": 430,
          "cds_start": 836,
          "cds_end": null,
          "cds_length": 1293,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
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      ],
      "gene_symbol": "ETV4",
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      "dbsnp": "rs141569304",
      "frequency_reference_population": 0.00007745281,
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      "allele_count_reference_population": 125,
      "gnomad_exomes_af": 0.0000499405,
      "gnomad_genomes_af": 0.000341777,
      "gnomad_exomes_ac": 73,
      "gnomad_genomes_ac": 52,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.09051164984703064,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.319,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.0868,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.29,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 8.159,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001986.4",
          "gene_symbol": "ETV4",
          "hgnc_id": 3493,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.998C>T",
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        {
          "score": -6,
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          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001322219.2",
          "gene_symbol": "DHX8",
          "hgnc_id": 2749,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.3444-6778G>A",
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}