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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-50375732-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=50375732&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "17",
      "pos": 50375732,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001166131.2",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.524C>T",
          "hgvs_p": "p.Thr175Ile",
          "transcript": "NM_152463.4",
          "protein_id": "NP_689676.2",
          "transcript_support_level": null,
          "aa_start": 175,
          "aa_end": null,
          "aa_length": 570,
          "cds_start": 524,
          "cds_end": null,
          "cds_length": 1713,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000338165.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_152463.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.524C>T",
          "hgvs_p": "p.Thr175Ile",
          "transcript": "ENST00000338165.9",
          "protein_id": "ENSP00000339897.4",
          "transcript_support_level": 2,
          "aa_start": 175,
          "aa_end": null,
          "aa_length": 570,
          "cds_start": 524,
          "cds_end": null,
          "cds_length": 1713,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_152463.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000338165.9"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.524C>T",
          "hgvs_p": "p.Thr175Ile",
          "transcript": "ENST00000511648.6",
          "protein_id": "ENSP00000421700.2",
          "transcript_support_level": 1,
          "aa_start": 175,
          "aa_end": null,
          "aa_length": 583,
          "cds_start": 524,
          "cds_end": null,
          "cds_length": 1752,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000511648.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "n.352+220C>T",
          "hgvs_p": null,
          "transcript": "ENST00000510007.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000510007.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.524C>T",
          "hgvs_p": "p.Thr175Ile",
          "transcript": "NM_001166131.2",
          "protein_id": "NP_001159603.1",
          "transcript_support_level": null,
          "aa_start": 175,
          "aa_end": null,
          "aa_length": 583,
          "cds_start": 524,
          "cds_end": null,
          "cds_length": 1752,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001166131.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.524C>T",
          "hgvs_p": "p.Thr175Ile",
          "transcript": "ENST00000393271.6",
          "protein_id": "ENSP00000376952.2",
          "transcript_support_level": 5,
          "aa_start": 175,
          "aa_end": null,
          "aa_length": 583,
          "cds_start": 524,
          "cds_end": null,
          "cds_length": 1752,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000393271.6"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.524C>T",
          "hgvs_p": "p.Thr175Ile",
          "transcript": "ENST00000927718.1",
          "protein_id": "ENSP00000597777.1",
          "transcript_support_level": null,
          "aa_start": 175,
          "aa_end": null,
          "aa_length": 566,
          "cds_start": 524,
          "cds_end": null,
          "cds_length": 1701,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000927718.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.524C>T",
          "hgvs_p": "p.Thr175Ile",
          "transcript": "ENST00000927717.1",
          "protein_id": "ENSP00000597776.1",
          "transcript_support_level": null,
          "aa_start": 175,
          "aa_end": null,
          "aa_length": 461,
          "cds_start": 524,
          "cds_end": null,
          "cds_length": 1386,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000927717.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.44C>T",
          "hgvs_p": "p.Thr15Ile",
          "transcript": "ENST00000510246.1",
          "protein_id": "ENSP00000425567.1",
          "transcript_support_level": 5,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 335,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 1009,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000510246.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.524C>T",
          "hgvs_p": "p.Thr175Ile",
          "transcript": "XM_047435470.1",
          "protein_id": "XP_047291426.1",
          "transcript_support_level": null,
          "aa_start": 175,
          "aa_end": null,
          "aa_length": 570,
          "cds_start": 524,
          "cds_end": null,
          "cds_length": 1713,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047435470.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.524C>T",
          "hgvs_p": "p.Thr175Ile",
          "transcript": "XM_017024236.3",
          "protein_id": "XP_016879725.1",
          "transcript_support_level": null,
          "aa_start": 175,
          "aa_end": null,
          "aa_length": 541,
          "cds_start": 524,
          "cds_end": null,
          "cds_length": 1626,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017024236.3"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.524C>T",
          "hgvs_p": "p.Thr175Ile",
          "transcript": "XM_047435471.1",
          "protein_id": "XP_047291427.1",
          "transcript_support_level": null,
          "aa_start": 175,
          "aa_end": null,
          "aa_length": 456,
          "cds_start": 524,
          "cds_end": null,
          "cds_length": 1371,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047435471.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LRRC59",
          "gene_hgnc_id": 28817,
          "hgvs_c": "c.168G>A",
          "hgvs_p": "p.Arg56Arg",
          "transcript": "ENST00000503118.2",
          "protein_id": "ENSP00000459616.1",
          "transcript_support_level": 5,
          "aa_start": 56,
          "aa_end": null,
          "aa_length": 56,
          "cds_start": 168,
          "cds_end": null,
          "cds_length": 171,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000503118.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.-523C>T",
          "hgvs_p": null,
          "transcript": "XM_047435472.1",
          "protein_id": "XP_047291428.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 247,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 744,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047435472.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.304+220C>T",
          "hgvs_p": null,
          "transcript": "ENST00000948328.1",
          "protein_id": "ENSP00000618387.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 413,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1242,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000948328.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.304+220C>T",
          "hgvs_p": null,
          "transcript": "ENST00000927716.1",
          "protein_id": "ENSP00000597775.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 384,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1155,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000927716.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.304+220C>T",
          "hgvs_p": null,
          "transcript": "ENST00000511519.6",
          "protein_id": "ENSP00000423029.2",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 170,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 514,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000511519.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "c.304+220C>T",
          "hgvs_p": null,
          "transcript": "XM_005257081.4",
          "protein_id": "XP_005257138.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 413,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1242,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005257081.4"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "n.597C>T",
          "hgvs_p": null,
          "transcript": "XR_007065270.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "XR_007065270.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "EME1",
          "gene_hgnc_id": 24965,
          "hgvs_c": "n.305-65C>T",
          "hgvs_p": null,
          "transcript": "ENST00000511711.5",
          "protein_id": "ENSP00000427392.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000511711.5"
        }
      ],
      "gene_symbol": "EME1",
      "gene_hgnc_id": 24965,
      "dbsnp": "rs746804207",
      "frequency_reference_population": 0.000023543202,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 38,
      "gnomad_exomes_af": 0.0000225739,
      "gnomad_genomes_af": 0.0000328537,
      "gnomad_exomes_ac": 33,
      "gnomad_genomes_ac": 5,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.04446730017662048,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.018,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0956,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.61,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.523,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001166131.2",
          "gene_symbol": "EME1",
          "hgnc_id": 24965,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.524C>T",
          "hgvs_p": "p.Thr175Ile"
        },
        {
          "score": -5,
          "benign_score": 5,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP7"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000503118.2",
          "gene_symbol": "LRRC59",
          "hgnc_id": 28817,
          "effects": [
            "synonymous_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.168G>A",
          "hgvs_p": "p.Arg56Arg"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}