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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-58206158-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=58206158&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "17",
      "pos": 58206158,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_017777.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.1601G>A",
          "hgvs_p": "p.Arg534Gln",
          "transcript": "NM_017777.4",
          "protein_id": "NP_060247.2",
          "transcript_support_level": null,
          "aa_start": 534,
          "aa_end": null,
          "aa_length": 559,
          "cds_start": 1601,
          "cds_end": null,
          "cds_length": 1680,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000393119.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_017777.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.1601G>A",
          "hgvs_p": "p.Arg534Gln",
          "transcript": "ENST00000393119.7",
          "protein_id": "ENSP00000376827.2",
          "transcript_support_level": 1,
          "aa_start": 534,
          "aa_end": null,
          "aa_length": 559,
          "cds_start": 1601,
          "cds_end": null,
          "cds_length": 1680,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_017777.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000393119.7"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.1172G>A",
          "hgvs_p": "p.Arg391Gln",
          "transcript": "ENST00000537529.7",
          "protein_id": "ENSP00000442096.3",
          "transcript_support_level": 1,
          "aa_start": 391,
          "aa_end": null,
          "aa_length": 416,
          "cds_start": 1172,
          "cds_end": null,
          "cds_length": 1251,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000537529.7"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.1634G>A",
          "hgvs_p": "p.Arg545Gln",
          "transcript": "ENST00000966002.1",
          "protein_id": "ENSP00000636061.1",
          "transcript_support_level": null,
          "aa_start": 545,
          "aa_end": null,
          "aa_length": 570,
          "cds_start": 1634,
          "cds_end": null,
          "cds_length": 1713,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000966002.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.1577G>A",
          "hgvs_p": "p.Arg526Gln",
          "transcript": "ENST00000872459.1",
          "protein_id": "ENSP00000542518.1",
          "transcript_support_level": null,
          "aa_start": 526,
          "aa_end": null,
          "aa_length": 551,
          "cds_start": 1577,
          "cds_end": null,
          "cds_length": 1656,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872459.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.1544G>A",
          "hgvs_p": "p.Arg515Gln",
          "transcript": "ENST00000872460.1",
          "protein_id": "ENSP00000542519.1",
          "transcript_support_level": null,
          "aa_start": 515,
          "aa_end": null,
          "aa_length": 540,
          "cds_start": 1544,
          "cds_end": null,
          "cds_length": 1623,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000872460.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.1472G>A",
          "hgvs_p": "p.Arg491Gln",
          "transcript": "ENST00000676787.1",
          "protein_id": "ENSP00000503999.1",
          "transcript_support_level": null,
          "aa_start": 491,
          "aa_end": null,
          "aa_length": 516,
          "cds_start": 1472,
          "cds_end": null,
          "cds_length": 1551,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000676787.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.992G>A",
          "hgvs_p": "p.Arg331Gln",
          "transcript": "NM_001321268.2",
          "protein_id": "NP_001308197.1",
          "transcript_support_level": null,
          "aa_start": 331,
          "aa_end": null,
          "aa_length": 356,
          "cds_start": 992,
          "cds_end": null,
          "cds_length": 1071,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001321268.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.1610G>A",
          "hgvs_p": "p.Arg537Gln",
          "transcript": "XM_011524958.3",
          "protein_id": "XP_011523260.1",
          "transcript_support_level": null,
          "aa_start": 537,
          "aa_end": null,
          "aa_length": 562,
          "cds_start": 1610,
          "cds_end": null,
          "cds_length": 1689,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011524958.3"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.1518G>A",
          "hgvs_p": "p.Ser506Ser",
          "transcript": "NM_001321269.2",
          "protein_id": "NP_001308198.1",
          "transcript_support_level": null,
          "aa_start": 506,
          "aa_end": null,
          "aa_length": 561,
          "cds_start": 1518,
          "cds_end": null,
          "cds_length": 1686,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001321269.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.1518G>A",
          "hgvs_p": "p.Ser506Ser",
          "transcript": "ENST00000678463.1",
          "protein_id": "ENSP00000502984.1",
          "transcript_support_level": null,
          "aa_start": 506,
          "aa_end": null,
          "aa_length": 561,
          "cds_start": 1518,
          "cds_end": null,
          "cds_length": 1686,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000678463.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.1527G>A",
          "hgvs_p": "p.Ser509Ser",
          "transcript": "XM_011524957.3",
          "protein_id": "XP_011523259.1",
          "transcript_support_level": null,
          "aa_start": 509,
          "aa_end": null,
          "aa_length": 564,
          "cds_start": 1527,
          "cds_end": null,
          "cds_length": 1695,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011524957.3"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.1089G>A",
          "hgvs_p": "p.Ser363Ser",
          "transcript": "XM_005257485.5",
          "protein_id": "XP_005257542.1",
          "transcript_support_level": null,
          "aa_start": 363,
          "aa_end": null,
          "aa_length": 418,
          "cds_start": 1089,
          "cds_end": null,
          "cds_length": 1257,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005257485.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.909G>A",
          "hgvs_p": "p.Ser303Ser",
          "transcript": "XM_047436333.1",
          "protein_id": "XP_047292289.1",
          "transcript_support_level": null,
          "aa_start": 303,
          "aa_end": null,
          "aa_length": 358,
          "cds_start": 909,
          "cds_end": null,
          "cds_length": 1077,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047436333.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.*13G>A",
          "hgvs_p": null,
          "transcript": "NM_001330397.2",
          "protein_id": "NP_001317326.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 456,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1371,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001330397.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "c.*13G>A",
          "hgvs_p": null,
          "transcript": "ENST00000313863.11",
          "protein_id": "ENSP00000316631.6",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 456,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1371,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000313863.11"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "n.*1008G>A",
          "hgvs_p": null,
          "transcript": "ENST00000393120.6",
          "protein_id": "ENSP00000376828.2",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000393120.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "n.427G>A",
          "hgvs_p": null,
          "transcript": "ENST00000583577.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000583577.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "n.*1220G>A",
          "hgvs_p": null,
          "transcript": "ENST00000675753.2",
          "protein_id": "ENSP00000502156.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000675753.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MKS1",
          "gene_hgnc_id": 7121,
          "hgvs_c": "n.*1075G>A",
          "hgvs_p": null,
          "transcript": "ENST00000677111.1",
          "protein_id": "ENSP00000504282.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000677111.1"
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      "dbsnp": "rs199910690",
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      "hom_count_reference_population": 0,
      "allele_count_reference_population": 107,
      "gnomad_exomes_af": 0.0000581536,
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      "gnomad_exomes_ac": 85,
      "gnomad_genomes_ac": 22,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.01651272177696228,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.711,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.1064,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.11,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 3.096,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
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      "apogee2_score": null,
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      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong,BP6",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 5,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6"
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          "verdict": "Likely_benign",
          "transcript": "NM_017777.4",
          "gene_symbol": "MKS1",
          "hgnc_id": 7121,
          "effects": [
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          "inheritance_mode": "AR,Unknown",
          "hgvs_c": "c.1601G>A",
          "hgvs_p": "p.Arg534Gln"
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      ],
      "clinvar_disease": " type 1,Bardet-Biedl syndrome 13,Joubert syndrome,Joubert syndrome 28,MKS1-related disorder,Meckel syndrome,Meckel-Gruber syndrome,Microcephaly,not provided,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:3 LB:2",
      "phenotype_combined": "Bardet-Biedl syndrome 13|not provided|not specified|Meckel-Gruber syndrome;Joubert syndrome|Microcephaly|MKS1-related disorder|Meckel syndrome, type 1|Joubert syndrome 28;Bardet-Biedl syndrome 13;Meckel syndrome, type 1",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}