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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-58521809-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=58521809&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "message": null,
  "variants": [
    {
      "acmg_by_gene": [
        {
          "benign_score": 1,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "effects": [
            "missense_variant"
          ],
          "gene_symbol": "SEPTIN4",
          "hgnc_id": 9165,
          "hgvs_c": "c.2396G>A",
          "hgvs_p": "p.Arg799Gln",
          "inheritance_mode": "AR",
          "pathogenic_score": 2,
          "score": 1,
          "transcript": "NM_001368771.2",
          "verdict": "Uncertain_significance"
        },
        {
          "benign_score": 1,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "effects": [
            "intron_variant"
          ],
          "gene_symbol": "SEPTIN4-AS1",
          "hgnc_id": 51345,
          "hgvs_c": "n.143+1832C>T",
          "hgvs_p": null,
          "inheritance_mode": "",
          "pathogenic_score": 2,
          "score": 1,
          "transcript": "ENST00000580589.6",
          "verdict": "Uncertain_significance"
        }
      ],
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4",
      "acmg_score": 1,
      "allele_count_reference_population": 16,
      "alphamissense_prediction": null,
      "alphamissense_score": 0.2107,
      "alt": "T",
      "apogee2_prediction": null,
      "apogee2_score": null,
      "bayesdelnoaf_prediction": "Benign",
      "bayesdelnoaf_score": -0.34,
      "chr": "17",
      "clinvar_classification": "Uncertain significance",
      "clinvar_disease": "not specified",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "computational_prediction_selected": "Benign",
      "computational_score_selected": 0.3368871212005615,
      "computational_source_selected": "MetaRNN",
      "consequences": [
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 996,
          "aa_ref": "R",
          "aa_start": 799,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 3303,
          "cdna_start": 2538,
          "cds_end": null,
          "cds_length": 2991,
          "cds_start": 2396,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 14,
          "exon_rank": 9,
          "exon_rank_end": null,
          "feature": "NM_001368771.2",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.2396G>A",
          "hgvs_p": "p.Arg799Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "ENST00000672673.2",
          "protein_coding": true,
          "protein_id": "NP_001355700.1",
          "strand": false,
          "transcript": "NM_001368771.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 996,
          "aa_ref": "R",
          "aa_start": 799,
          "biotype": "protein_coding",
          "canonical": true,
          "cdna_end": null,
          "cdna_length": 3303,
          "cdna_start": 2538,
          "cds_end": null,
          "cds_length": 2991,
          "cds_start": 2396,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 14,
          "exon_rank": 9,
          "exon_rank_end": null,
          "feature": "ENST00000672673.2",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.2396G>A",
          "hgvs_p": "p.Arg799Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": "NM_001368771.2",
          "protein_coding": true,
          "protein_id": "ENSP00000500383.1",
          "strand": false,
          "transcript": "ENST00000672673.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 478,
          "aa_ref": "R",
          "aa_start": 281,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1784,
          "cdna_start": 1019,
          "cds_end": null,
          "cds_length": 1437,
          "cds_start": 842,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 12,
          "exon_rank": 7,
          "exon_rank_end": null,
          "feature": "ENST00000317268.7",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.842G>A",
          "hgvs_p": "p.Arg281Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000321674.3",
          "strand": false,
          "transcript": "ENST00000317268.7",
          "transcript_support_level": 1
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 459,
          "aa_ref": "R",
          "aa_start": 262,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1530,
          "cdna_start": 868,
          "cds_end": null,
          "cds_length": 1380,
          "cds_start": 785,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 12,
          "exon_rank": 7,
          "exon_rank_end": null,
          "feature": "ENST00000317256.10",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.785G>A",
          "hgvs_p": "p.Arg262Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000321071.6",
          "strand": false,
          "transcript": "ENST00000317256.10",
          "transcript_support_level": 1
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 274,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1906,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 825,
          "cds_start": null,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_count": 10,
          "exon_rank": 6,
          "exon_rank_end": null,
          "feature": "ENST00000426861.5",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.*73G>A",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000402348.1",
          "strand": false,
          "transcript": "ENST00000426861.5",
          "transcript_support_level": 1
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": 175,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 952,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": 528,
          "cds_start": null,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_count": 5,
          "exon_rank": 5,
          "exon_rank_end": null,
          "feature": "ENST00000580809.5",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.*73G>A",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000464382.1",
          "strand": false,
          "transcript": "ENST00000580809.5",
          "transcript_support_level": 1
        },
        {
          "aa_alt": null,
          "aa_end": null,
          "aa_length": null,
          "aa_ref": null,
          "aa_start": null,
          "biotype": "retained_intron",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1731,
          "cdna_start": null,
          "cds_end": null,
          "cds_length": null,
          "cds_start": null,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_count": 11,
          "exon_rank": 7,
          "exon_rank_end": null,
          "feature": "ENST00000584488.5",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "n.981G>A",
          "hgvs_p": null,
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": false,
          "protein_id": null,
          "strand": false,
          "transcript": "ENST00000584488.5",
          "transcript_support_level": 1
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 493,
          "aa_ref": "R",
          "aa_start": 296,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1797,
          "cdna_start": 1032,
          "cds_end": null,
          "cds_length": 1482,
          "cds_start": 887,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 13,
          "exon_rank": 8,
          "exon_rank_end": null,
          "feature": "NM_001256782.2",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.887G>A",
          "hgvs_p": "p.Arg296Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_001243711.1",
          "strand": false,
          "transcript": "NM_001256782.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 493,
          "aa_ref": "R",
          "aa_start": 296,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1795,
          "cdna_start": 1032,
          "cds_end": null,
          "cds_length": 1482,
          "cds_start": 887,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 13,
          "exon_rank": 8,
          "exon_rank_end": null,
          "feature": "ENST00000457347.6",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.887G>A",
          "hgvs_p": "p.Arg296Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000402000.2",
          "strand": false,
          "transcript": "ENST00000457347.6",
          "transcript_support_level": 2
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 493,
          "aa_ref": "R",
          "aa_start": 296,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1795,
          "cdna_start": 1032,
          "cds_end": null,
          "cds_length": 1482,
          "cds_start": 887,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 13,
          "exon_rank": 8,
          "exon_rank_end": null,
          "feature": "ENST00000672699.1",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.887G>A",
          "hgvs_p": "p.Arg296Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000500355.1",
          "strand": false,
          "transcript": "ENST00000672699.1",
          "transcript_support_level": null
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 478,
          "aa_ref": "R",
          "aa_start": 281,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1749,
          "cdna_start": 984,
          "cds_end": null,
          "cds_length": 1437,
          "cds_start": 842,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 12,
          "exon_rank": 7,
          "exon_rank_end": null,
          "feature": "NM_004574.5",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.842G>A",
          "hgvs_p": "p.Arg281Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_004565.1",
          "strand": false,
          "transcript": "NM_004574.5",
          "transcript_support_level": null
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 471,
          "aa_ref": "R",
          "aa_start": 274,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1658,
          "cdna_start": 893,
          "cds_end": null,
          "cds_length": 1416,
          "cds_start": 821,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 14,
          "exon_rank": 9,
          "exon_rank_end": null,
          "feature": "NM_001368772.2",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.821G>A",
          "hgvs_p": "p.Arg274Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_001355701.1",
          "strand": false,
          "transcript": "NM_001368772.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 470,
          "aa_ref": "R",
          "aa_start": 273,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1700,
          "cdna_start": 935,
          "cds_end": null,
          "cds_length": 1413,
          "cds_start": 818,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 12,
          "exon_rank": 7,
          "exon_rank_end": null,
          "feature": "NM_001198713.2",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.818G>A",
          "hgvs_p": "p.Arg273Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_001185642.1",
          "strand": false,
          "transcript": "NM_001198713.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 470,
          "aa_ref": "R",
          "aa_start": 273,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1597,
          "cdna_start": 935,
          "cds_end": null,
          "cds_length": 1413,
          "cds_start": 818,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 12,
          "exon_rank": 7,
          "exon_rank_end": null,
          "feature": "ENST00000412945.7",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.818G>A",
          "hgvs_p": "p.Arg273Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000414779.3",
          "strand": false,
          "transcript": "ENST00000412945.7",
          "transcript_support_level": 2
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 459,
          "aa_ref": "R",
          "aa_start": 262,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1647,
          "cdna_start": 882,
          "cds_end": null,
          "cds_length": 1380,
          "cds_start": 785,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 12,
          "exon_rank": 7,
          "exon_rank_end": null,
          "feature": "NM_080416.4",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.785G>A",
          "hgvs_p": "p.Arg262Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_536341.1",
          "strand": false,
          "transcript": "NM_080416.4",
          "transcript_support_level": null
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 459,
          "aa_ref": "R",
          "aa_start": 262,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1556,
          "cdna_start": 785,
          "cds_end": null,
          "cds_length": 1380,
          "cds_start": 785,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 12,
          "exon_rank": 7,
          "exon_rank_end": null,
          "feature": "ENST00000393086.5",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.785G>A",
          "hgvs_p": "p.Arg262Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000376801.1",
          "strand": false,
          "transcript": "ENST00000393086.5",
          "transcript_support_level": 5
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 379,
          "aa_ref": "R",
          "aa_start": 182,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1452,
          "cdna_start": 687,
          "cds_end": null,
          "cds_length": 1140,
          "cds_start": 545,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 11,
          "exon_rank": 6,
          "exon_rank_end": null,
          "feature": "NM_001363803.2",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.545G>A",
          "hgvs_p": "p.Arg182Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "NP_001350732.1",
          "strand": false,
          "transcript": "NM_001363803.2",
          "transcript_support_level": null
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 379,
          "aa_ref": "R",
          "aa_start": 182,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1396,
          "cdna_start": 674,
          "cds_end": null,
          "cds_length": 1140,
          "cds_start": 545,
          "consequences": [
            "missense_variant"
          ],
          "exon_count": 11,
          "exon_rank": 6,
          "exon_rank_end": null,
          "feature": "ENST00000580844.5",
          "gene_hgnc_id": 9165,
          "gene_symbol": "SEPTIN4",
          "hgvs_c": "c.545G>A",
          "hgvs_p": "p.Arg182Gln",
          "intron_rank": null,
          "intron_rank_end": null,
          "mane_plus": null,
          "mane_select": null,
          "protein_coding": true,
          "protein_id": "ENSP00000462727.1",
          "strand": false,
          "transcript": "ENST00000580844.5",
          "transcript_support_level": 5
        },
        {
          "aa_alt": "Q",
          "aa_end": null,
          "aa_length": 332,
          "aa_ref": "R",
          "aa_start": 182,
          "biotype": "protein_coding",
          "canonical": false,
          "cdna_end": null,
          "cdna_length": 1362,
          "cdna_start": 650,
          "cds_end": null,
          "cds_length": 999,
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      "dbscsnv_ada_prediction": null,
      "dbscsnv_ada_score": null,
      "dbsnp": "rs776786457",
      "effect": "missense_variant",
      "frequency_reference_population": 0.000009912854,
      "gene_hgnc_id": 9165,
      "gene_symbol": "SEPTIN4",
      "gnomad_exomes_ac": 10,
      "gnomad_exomes_af": 0.00000684047,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_ac": 6,
      "gnomad_genomes_af": 0.0000394275,
      "gnomad_genomes_homalt": 1,
      "gnomad_mito_heteroplasmic": null,
      "gnomad_mito_homoplasmic": null,
      "hom_count_reference_population": 1,
      "mitotip_prediction": null,
      "mitotip_score": null,
      "pathogenicity_classification_combined": "Uncertain significance",
      "phenotype_combined": "not specified",
      "phylop100way_prediction": "Benign",
      "phylop100way_score": 2.645,
      "pos": 58521809,
      "ref": "C",
      "revel_prediction": "Benign",
      "revel_score": 0.137,
      "splice_prediction_selected": "Benign",
      "splice_score_selected": 0.05999999865889549,
      "splice_source_selected": "max_spliceai",
      "spliceai_max_prediction": "Benign",
      "spliceai_max_score": 0.06,
      "transcript": "NM_001368771.2"
    }
  ]
}
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