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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-59575424-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=59575424&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "17",
      "pos": 59575424,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_024612.5",
      "consequences": [
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.926C>T",
          "hgvs_p": "p.Thr309Ile",
          "transcript": "NM_024612.5",
          "protein_id": "NP_078888.4",
          "transcript_support_level": null,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 779,
          "cds_start": 926,
          "cds_end": null,
          "cds_length": 2340,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000251241.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_024612.5"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.926C>T",
          "hgvs_p": "p.Thr309Ile",
          "transcript": "ENST00000251241.9",
          "protein_id": "ENSP00000251241.4",
          "transcript_support_level": 1,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 779,
          "cds_start": 926,
          "cds_end": null,
          "cds_length": 2340,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_024612.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000251241.9"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.926C>T",
          "hgvs_p": "p.Thr309Ile",
          "transcript": "ENST00000930678.1",
          "protein_id": "ENSP00000600737.1",
          "transcript_support_level": null,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 779,
          "cds_start": 926,
          "cds_end": null,
          "cds_length": 2340,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930678.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.923C>T",
          "hgvs_p": "p.Thr308Ile",
          "transcript": "ENST00000930680.1",
          "protein_id": "ENSP00000600739.1",
          "transcript_support_level": null,
          "aa_start": 308,
          "aa_end": null,
          "aa_length": 778,
          "cds_start": 923,
          "cds_end": null,
          "cds_length": 2337,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930680.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.926C>T",
          "hgvs_p": "p.Thr309Ile",
          "transcript": "ENST00000961853.1",
          "protein_id": "ENSP00000631912.1",
          "transcript_support_level": null,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 778,
          "cds_start": 926,
          "cds_end": null,
          "cds_length": 2337,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000961853.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.926C>T",
          "hgvs_p": "p.Thr309Ile",
          "transcript": "ENST00000930679.1",
          "protein_id": "ENSP00000600738.1",
          "transcript_support_level": null,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 764,
          "cds_start": 926,
          "cds_end": null,
          "cds_length": 2295,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000930679.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.926C>T",
          "hgvs_p": "p.Thr309Ile",
          "transcript": "ENST00000961854.1",
          "protein_id": "ENSP00000631913.1",
          "transcript_support_level": null,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 750,
          "cds_start": 926,
          "cds_end": null,
          "cds_length": 2253,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000961854.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.806C>T",
          "hgvs_p": "p.Thr269Ile",
          "transcript": "ENST00000961857.1",
          "protein_id": "ENSP00000631916.1",
          "transcript_support_level": null,
          "aa_start": 269,
          "aa_end": null,
          "aa_length": 739,
          "cds_start": 806,
          "cds_end": null,
          "cds_length": 2220,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000961857.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.779C>T",
          "hgvs_p": "p.Thr260Ile",
          "transcript": "ENST00000961856.1",
          "protein_id": "ENSP00000631915.1",
          "transcript_support_level": null,
          "aa_start": 260,
          "aa_end": null,
          "aa_length": 730,
          "cds_start": 779,
          "cds_end": null,
          "cds_length": 2193,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000961856.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.773C>T",
          "hgvs_p": "p.Thr258Ile",
          "transcript": "ENST00000867586.1",
          "protein_id": "ENSP00000537645.1",
          "transcript_support_level": null,
          "aa_start": 258,
          "aa_end": null,
          "aa_length": 728,
          "cds_start": 773,
          "cds_end": null,
          "cds_length": 2187,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000867586.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.926C>T",
          "hgvs_p": "p.Thr309Ile",
          "transcript": "ENST00000961852.1",
          "protein_id": "ENSP00000631911.1",
          "transcript_support_level": null,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 718,
          "cds_start": 926,
          "cds_end": null,
          "cds_length": 2157,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000961852.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.695C>T",
          "hgvs_p": "p.Thr232Ile",
          "transcript": "NM_001166301.2",
          "protein_id": "NP_001159773.1",
          "transcript_support_level": null,
          "aa_start": 232,
          "aa_end": null,
          "aa_length": 702,
          "cds_start": 695,
          "cds_end": null,
          "cds_length": 2109,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001166301.2"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.695C>T",
          "hgvs_p": "p.Thr232Ile",
          "transcript": "ENST00000425628.7",
          "protein_id": "ENSP00000388749.3",
          "transcript_support_level": 2,
          "aa_start": 232,
          "aa_end": null,
          "aa_length": 702,
          "cds_start": 695,
          "cds_end": null,
          "cds_length": 2109,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000425628.7"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.665C>T",
          "hgvs_p": "p.Thr222Ile",
          "transcript": "ENST00000930681.1",
          "protein_id": "ENSP00000600740.1",
          "transcript_support_level": null,
          "aa_start": 222,
          "aa_end": null,
          "aa_length": 692,
          "cds_start": 665,
          "cds_end": null,
          "cds_length": 2079,
          "cdna_start": null,
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          "feature": "ENST00000930681.1"
        },
        {
          "aa_ref": "T",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 5,
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          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.578C>T",
          "hgvs_p": "p.Thr193Ile",
          "transcript": "ENST00000961855.1",
          "protein_id": "ENSP00000631914.1",
          "transcript_support_level": null,
          "aa_start": 193,
          "aa_end": null,
          "aa_length": 663,
          "cds_start": 578,
          "cds_end": null,
          "cds_length": 1992,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000961855.1"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.926C>T",
          "hgvs_p": "p.Thr309Ile",
          "transcript": "XM_006722083.4",
          "protein_id": "XP_006722146.1",
          "transcript_support_level": null,
          "aa_start": 309,
          "aa_end": null,
          "aa_length": 752,
          "cds_start": 926,
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          "cds_length": 2259,
          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "XM_006722083.4"
        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.758C>T",
          "hgvs_p": "p.Thr253Ile",
          "transcript": "XM_017025080.3",
          "protein_id": "XP_016880569.1",
          "transcript_support_level": null,
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          "aa_length": 723,
          "cds_start": 758,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "T",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
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          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.926C>T",
          "hgvs_p": "p.Thr309Ile",
          "transcript": "XM_011525253.4",
          "protein_id": "XP_011523555.1",
          "transcript_support_level": null,
          "aa_start": 309,
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          "cds_start": 926,
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          "cds_length": 2157,
          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "T",
          "aa_alt": "I",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.758C>T",
          "hgvs_p": "p.Thr253Ile",
          "transcript": "XM_047436758.1",
          "protein_id": "XP_047292714.1",
          "transcript_support_level": null,
          "aa_start": 253,
          "aa_end": null,
          "aa_length": 662,
          "cds_start": 758,
          "cds_end": null,
          "cds_length": 1989,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047436758.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DHX40",
          "gene_hgnc_id": 18018,
          "hgvs_c": "c.*60C>T",
          "hgvs_p": null,
          "transcript": "ENST00000580030.1",
          "protein_id": "ENSP00000462686.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 267,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 806,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000580030.1"
        }
      ],
      "gene_symbol": "DHX40",
      "gene_hgnc_id": 18018,
      "dbsnp": "rs2523376",
      "frequency_reference_population": 0.000006570993,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 0.00000547687,
      "gnomad_genomes_af": 0.00000657099,
      "gnomad_exomes_ac": 8,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.21914082765579224,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.116,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.3678,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.53,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.552,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_024612.5",
          "gene_symbol": "DHX40",
          "hgnc_id": 18018,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.926C>T",
          "hgvs_p": "p.Thr309Ile"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}