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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-74704696-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=74704696&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "17",
      "pos": 74704696,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001289084.2",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.164G>A",
          "hgvs_p": "p.Arg55Gln",
          "transcript": "NM_139018.5",
          "protein_id": "NP_620587.2",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 290,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 873,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000326165.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_139018.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.164G>A",
          "hgvs_p": "p.Arg55Gln",
          "transcript": "ENST00000326165.11",
          "protein_id": "ENSP00000327075.6",
          "transcript_support_level": 1,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 290,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 873,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_139018.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000326165.11"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.173G>A",
          "hgvs_p": "p.Arg58Gln",
          "transcript": "ENST00000464910.5",
          "protein_id": "ENSP00000464257.1",
          "transcript_support_level": 1,
          "aa_start": 58,
          "aa_end": null,
          "aa_length": 293,
          "cds_start": 173,
          "cds_end": null,
          "cds_length": 882,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000464910.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.173G>A",
          "hgvs_p": "p.Arg58Gln",
          "transcript": "ENST00000581500.1",
          "protein_id": "ENSP00000464610.1",
          "transcript_support_level": 1,
          "aa_start": 58,
          "aa_end": null,
          "aa_length": 239,
          "cds_start": 173,
          "cds_end": null,
          "cds_length": 720,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000581500.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.173G>A",
          "hgvs_p": "p.Arg58Gln",
          "transcript": "ENST00000469092.5",
          "protein_id": "ENSP00000463743.1",
          "transcript_support_level": 1,
          "aa_start": 58,
          "aa_end": null,
          "aa_length": 194,
          "cds_start": 173,
          "cds_end": null,
          "cds_length": 585,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000469092.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "n.173G>A",
          "hgvs_p": null,
          "transcript": "ENST00000462044.5",
          "protein_id": "ENSP00000464223.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000462044.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.164G>A",
          "hgvs_p": "p.Arg55Gln",
          "transcript": "ENST00000963433.1",
          "protein_id": "ENSP00000633492.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 313,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 942,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963433.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.164G>A",
          "hgvs_p": "p.Arg55Gln",
          "transcript": "ENST00000963432.1",
          "protein_id": "ENSP00000633491.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 309,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 930,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963432.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.164G>A",
          "hgvs_p": "p.Arg55Gln",
          "transcript": "ENST00000886268.1",
          "protein_id": "ENSP00000556327.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 308,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 927,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000886268.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.164G>A",
          "hgvs_p": "p.Arg55Gln",
          "transcript": "NM_001289084.2",
          "protein_id": "NP_001276013.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 305,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 918,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001289084.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.164G>A",
          "hgvs_p": "p.Arg55Gln",
          "transcript": "ENST00000583937.5",
          "protein_id": "ENSP00000462309.1",
          "transcript_support_level": 2,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 305,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 918,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000583937.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.164G>A",
          "hgvs_p": "p.Arg55Gln",
          "transcript": "ENST00000963430.1",
          "protein_id": "ENSP00000633489.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 304,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 915,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963430.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.173G>A",
          "hgvs_p": "p.Arg58Gln",
          "transcript": "NM_001289085.2",
          "protein_id": "NP_001276014.1",
          "transcript_support_level": null,
          "aa_start": 58,
          "aa_end": null,
          "aa_length": 293,
          "cds_start": 173,
          "cds_end": null,
          "cds_length": 882,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001289085.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.164G>A",
          "hgvs_p": "p.Arg55Gln",
          "transcript": "ENST00000963431.1",
          "protein_id": "ENSP00000633490.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 289,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 870,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000963431.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.164G>A",
          "hgvs_p": "p.Arg55Gln",
          "transcript": "NM_001289083.2",
          "protein_id": "NP_001276012.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 244,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 735,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001289083.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.164G>A",
          "hgvs_p": "p.Arg55Gln",
          "transcript": "ENST00000301573.13",
          "protein_id": "ENSP00000301573.9",
          "transcript_support_level": 2,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 244,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 735,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000301573.13"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.173G>A",
          "hgvs_p": "p.Arg58Gln",
          "transcript": "NM_001289086.2",
          "protein_id": "NP_001276015.1",
          "transcript_support_level": null,
          "aa_start": 58,
          "aa_end": null,
          "aa_length": 239,
          "cds_start": 173,
          "cds_end": null,
          "cds_length": 720,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001289086.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.164G>A",
          "hgvs_p": "p.Arg55Gln",
          "transcript": "NM_001289087.2",
          "protein_id": "NP_001276016.1",
          "transcript_support_level": null,
          "aa_start": 55,
          "aa_end": null,
          "aa_length": 221,
          "cds_start": 164,
          "cds_end": null,
          "cds_length": 666,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001289087.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.173G>A",
          "hgvs_p": "p.Arg58Gln",
          "transcript": "NM_001289082.2",
          "protein_id": "NP_001276011.1",
          "transcript_support_level": null,
          "aa_start": 58,
          "aa_end": null,
          "aa_length": 194,
          "cds_start": 173,
          "cds_end": null,
          "cds_length": 585,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001289082.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CD300LF",
          "gene_hgnc_id": 29883,
          "hgvs_c": "c.173G>A",
          "hgvs_p": "p.Arg58Gln",
          "transcript": "XM_047435407.1",
          "protein_id": "XP_047291363.1",
          "transcript_support_level": null,
          "aa_start": 58,
          "aa_end": null,
          "aa_length": 338,
          "cds_start": 173,
          "cds_end": null,
          "cds_length": 1017,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047435407.1"
        },
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      "dbsnp": "rs199990109",
      "frequency_reference_population": 0.0002093919,
      "hom_count_reference_population": 1,
      "allele_count_reference_population": 338,
      "gnomad_exomes_af": 0.000210686,
      "gnomad_genomes_af": 0.000196969,
      "gnomad_exomes_ac": 308,
      "gnomad_genomes_ac": 30,
      "gnomad_exomes_homalt": 1,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.016047567129135132,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.348,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.1155,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.4,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.134,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001289084.2",
          "gene_symbol": "CD300LF",
          "hgnc_id": 29883,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.164G>A",
          "hgvs_p": "p.Arg55Gln"
        },
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_175738.5",
          "gene_symbol": "RAB37",
          "hgnc_id": 30268,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.73-24560C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}