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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-75628354-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=75628354&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "17",
      "pos": 75628354,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_004259.7",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.2669G>C",
          "hgvs_p": "p.Ser890Thr",
          "transcript": "NM_004259.7",
          "protein_id": "NP_004250.4",
          "transcript_support_level": null,
          "aa_start": 890,
          "aa_end": null,
          "aa_length": 991,
          "cds_start": 2669,
          "cds_end": null,
          "cds_length": 2976,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000317905.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004259.7"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.2669G>C",
          "hgvs_p": "p.Ser890Thr",
          "transcript": "ENST00000317905.10",
          "protein_id": "ENSP00000317636.5",
          "transcript_support_level": 1,
          "aa_start": 890,
          "aa_end": null,
          "aa_length": 991,
          "cds_start": 2669,
          "cds_end": null,
          "cds_length": 2976,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_004259.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000317905.10"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.2588G>C",
          "hgvs_p": "p.Ser863Thr",
          "transcript": "ENST00000423245.6",
          "protein_id": "ENSP00000394820.2",
          "transcript_support_level": 1,
          "aa_start": 863,
          "aa_end": null,
          "aa_length": 964,
          "cds_start": 2588,
          "cds_end": null,
          "cds_length": 2895,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000423245.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "n.2205G>C",
          "hgvs_p": null,
          "transcript": "ENST00000443199.6",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000443199.6"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "n.292G>C",
          "hgvs_p": null,
          "transcript": "ENST00000578865.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000578865.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.2669G>C",
          "hgvs_p": "p.Ser890Thr",
          "transcript": "ENST00000919295.1",
          "protein_id": "ENSP00000589354.1",
          "transcript_support_level": null,
          "aa_start": 890,
          "aa_end": null,
          "aa_length": 991,
          "cds_start": 2669,
          "cds_end": null,
          "cds_length": 2976,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919295.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.2669G>C",
          "hgvs_p": "p.Ser890Thr",
          "transcript": "ENST00000919296.1",
          "protein_id": "ENSP00000589355.1",
          "transcript_support_level": null,
          "aa_start": 890,
          "aa_end": null,
          "aa_length": 991,
          "cds_start": 2669,
          "cds_end": null,
          "cds_length": 2976,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919296.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.2669G>C",
          "hgvs_p": "p.Ser890Thr",
          "transcript": "ENST00000941132.1",
          "protein_id": "ENSP00000611191.1",
          "transcript_support_level": null,
          "aa_start": 890,
          "aa_end": null,
          "aa_length": 990,
          "cds_start": 2669,
          "cds_end": null,
          "cds_length": 2973,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000941132.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.2615G>C",
          "hgvs_p": "p.Ser872Thr",
          "transcript": "ENST00000910739.1",
          "protein_id": "ENSP00000580798.1",
          "transcript_support_level": null,
          "aa_start": 872,
          "aa_end": null,
          "aa_length": 973,
          "cds_start": 2615,
          "cds_end": null,
          "cds_length": 2922,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910739.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.2534G>C",
          "hgvs_p": "p.Ser845Thr",
          "transcript": "ENST00000941133.1",
          "protein_id": "ENSP00000611192.1",
          "transcript_support_level": null,
          "aa_start": 845,
          "aa_end": null,
          "aa_length": 946,
          "cds_start": 2534,
          "cds_end": null,
          "cds_length": 2841,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000941133.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.2333G>C",
          "hgvs_p": "p.Ser778Thr",
          "transcript": "ENST00000919294.1",
          "protein_id": "ENSP00000589353.1",
          "transcript_support_level": null,
          "aa_start": 778,
          "aa_end": null,
          "aa_length": 879,
          "cds_start": 2333,
          "cds_end": null,
          "cds_length": 2640,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919294.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.2270G>C",
          "hgvs_p": "p.Ser757Thr",
          "transcript": "ENST00000910741.1",
          "protein_id": "ENSP00000580800.1",
          "transcript_support_level": null,
          "aa_start": 757,
          "aa_end": null,
          "aa_length": 858,
          "cds_start": 2270,
          "cds_end": null,
          "cds_length": 2577,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910741.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.1871G>C",
          "hgvs_p": "p.Ser624Thr",
          "transcript": "ENST00000910740.1",
          "protein_id": "ENSP00000580799.1",
          "transcript_support_level": null,
          "aa_start": 624,
          "aa_end": null,
          "aa_length": 725,
          "cds_start": 1871,
          "cds_end": null,
          "cds_length": 2178,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000910740.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.341G>C",
          "hgvs_p": "p.Ser114Thr",
          "transcript": "ENST00000581825.1",
          "protein_id": "ENSP00000462678.1",
          "transcript_support_level": 3,
          "aa_start": 114,
          "aa_end": null,
          "aa_length": 205,
          "cds_start": 341,
          "cds_end": null,
          "cds_length": 618,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000581825.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.2732G>C",
          "hgvs_p": "p.Ser911Thr",
          "transcript": "XM_005257818.5",
          "protein_id": "XP_005257875.1",
          "transcript_support_level": null,
          "aa_start": 911,
          "aa_end": null,
          "aa_length": 1012,
          "cds_start": 2732,
          "cds_end": null,
          "cds_length": 3039,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005257818.5"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.2669G>C",
          "hgvs_p": "p.Ser890Thr",
          "transcript": "XM_047437085.1",
          "protein_id": "XP_047293041.1",
          "transcript_support_level": null,
          "aa_start": 890,
          "aa_end": null,
          "aa_length": 991,
          "cds_start": 2669,
          "cds_end": null,
          "cds_length": 2976,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047437085.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.1595G>C",
          "hgvs_p": "p.Ser532Thr",
          "transcript": "XM_047437086.1",
          "protein_id": "XP_047293042.1",
          "transcript_support_level": null,
          "aa_start": 532,
          "aa_end": null,
          "aa_length": 633,
          "cds_start": 1595,
          "cds_end": null,
          "cds_length": 1902,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047437086.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.1595G>C",
          "hgvs_p": "p.Ser532Thr",
          "transcript": "XM_047437087.1",
          "protein_id": "XP_047293043.1",
          "transcript_support_level": null,
          "aa_start": 532,
          "aa_end": null,
          "aa_length": 633,
          "cds_start": 1595,
          "cds_end": null,
          "cds_length": 1902,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047437087.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.1550G>C",
          "hgvs_p": "p.Ser517Thr",
          "transcript": "XM_011525484.2",
          "protein_id": "XP_011523786.1",
          "transcript_support_level": null,
          "aa_start": 517,
          "aa_end": null,
          "aa_length": 618,
          "cds_start": 1550,
          "cds_end": null,
          "cds_length": 1857,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011525484.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RECQL5",
          "gene_hgnc_id": 9950,
          "hgvs_c": "c.1532G>C",
          "hgvs_p": "p.Ser511Thr",
          "transcript": "XM_011525485.3",
          "protein_id": "XP_011523787.1",
          "transcript_support_level": null,
          "aa_start": 511,
          "aa_end": null,
          "aa_length": 612,
          "cds_start": 1532,
          "cds_end": null,
          "cds_length": 1839,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
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        {
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        {
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      ],
      "gene_symbol": "RECQL5",
      "gene_hgnc_id": 9950,
      "dbsnp": "rs928380682",
      "frequency_reference_population": 0.000008054923,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 13,
      "gnomad_exomes_af": 0.00000820948,
      "gnomad_genomes_af": 0.00000657047,
      "gnomad_exomes_ac": 12,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.09122732281684875,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.104,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0713,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.55,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.119,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_004259.7",
          "gene_symbol": "RECQL5",
          "hgnc_id": 9950,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR,AD",
          "hgvs_c": "c.2669G>C",
          "hgvs_p": "p.Ser890Thr"
        },
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000910959.1",
          "gene_symbol": "SMIM5",
          "hgnc_id": 40030,
          "effects": [
            "upstream_gene_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.-5888C>G",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}