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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-75891833-A-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=75891833&ref=A&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "17",
      "pos": 75891833,
      "ref": "A",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_173547.4",
      "consequences": [
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.965T>G",
          "hgvs_p": "p.Leu322Arg",
          "transcript": "NM_173547.4",
          "protein_id": "NP_775818.2",
          "transcript_support_level": null,
          "aa_start": 322,
          "aa_end": null,
          "aa_length": 517,
          "cds_start": 965,
          "cds_end": null,
          "cds_length": 1554,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000269383.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_173547.4"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.965T>G",
          "hgvs_p": "p.Leu322Arg",
          "transcript": "ENST00000269383.8",
          "protein_id": "ENSP00000269383.3",
          "transcript_support_level": 1,
          "aa_start": 322,
          "aa_end": null,
          "aa_length": 517,
          "cds_start": 965,
          "cds_end": null,
          "cds_length": 1554,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_173547.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000269383.8"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.1004T>G",
          "hgvs_p": "p.Leu335Arg",
          "transcript": "ENST00000924710.1",
          "protein_id": "ENSP00000594769.1",
          "transcript_support_level": null,
          "aa_start": 335,
          "aa_end": null,
          "aa_length": 530,
          "cds_start": 1004,
          "cds_end": null,
          "cds_length": 1593,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000924710.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.893T>G",
          "hgvs_p": "p.Leu298Arg",
          "transcript": "ENST00000924712.1",
          "protein_id": "ENSP00000594771.1",
          "transcript_support_level": null,
          "aa_start": 298,
          "aa_end": null,
          "aa_length": 493,
          "cds_start": 893,
          "cds_end": null,
          "cds_length": 1482,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000924712.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.869T>G",
          "hgvs_p": "p.Leu290Arg",
          "transcript": "ENST00000909232.1",
          "protein_id": "ENSP00000579291.1",
          "transcript_support_level": null,
          "aa_start": 290,
          "aa_end": null,
          "aa_length": 485,
          "cds_start": 869,
          "cds_end": null,
          "cds_length": 1458,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000909232.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.788T>G",
          "hgvs_p": "p.Leu263Arg",
          "transcript": "ENST00000946120.1",
          "protein_id": "ENSP00000616179.1",
          "transcript_support_level": null,
          "aa_start": 263,
          "aa_end": null,
          "aa_length": 458,
          "cds_start": 788,
          "cds_end": null,
          "cds_length": 1377,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000946120.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.257T>G",
          "hgvs_p": "p.Leu86Arg",
          "transcript": "ENST00000591668.5",
          "protein_id": "ENSP00000465034.1",
          "transcript_support_level": 2,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 136,
          "cds_start": 257,
          "cds_end": null,
          "cds_length": 411,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000591668.5"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.1136T>G",
          "hgvs_p": "p.Leu379Arg",
          "transcript": "XM_047435580.1",
          "protein_id": "XP_047291536.1",
          "transcript_support_level": null,
          "aa_start": 379,
          "aa_end": null,
          "aa_length": 574,
          "cds_start": 1136,
          "cds_end": null,
          "cds_length": 1725,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047435580.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.1097T>G",
          "hgvs_p": "p.Leu366Arg",
          "transcript": "XM_047435581.1",
          "protein_id": "XP_047291537.1",
          "transcript_support_level": null,
          "aa_start": 366,
          "aa_end": null,
          "aa_length": 561,
          "cds_start": 1097,
          "cds_end": null,
          "cds_length": 1686,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047435581.1"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.1004T>G",
          "hgvs_p": "p.Leu335Arg",
          "transcript": "XM_006721760.4",
          "protein_id": "XP_006721823.1",
          "transcript_support_level": null,
          "aa_start": 335,
          "aa_end": null,
          "aa_length": 530,
          "cds_start": 1004,
          "cds_end": null,
          "cds_length": 1593,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006721760.4"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.1004T>G",
          "hgvs_p": "p.Leu335Arg",
          "transcript": "XM_011524499.3",
          "protein_id": "XP_011522801.1",
          "transcript_support_level": null,
          "aa_start": 335,
          "aa_end": null,
          "aa_length": 385,
          "cds_start": 1004,
          "cds_end": null,
          "cds_length": 1158,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011524499.3"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.1004T>G",
          "hgvs_p": "p.Leu335Arg",
          "transcript": "XM_011524500.3",
          "protein_id": "XP_011522802.1",
          "transcript_support_level": null,
          "aa_start": 335,
          "aa_end": null,
          "aa_length": 385,
          "cds_start": 1004,
          "cds_end": null,
          "cds_length": 1158,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011524500.3"
        },
        {
          "aa_ref": "L",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.965T>G",
          "hgvs_p": "p.Leu322Arg",
          "transcript": "XM_047435583.1",
          "protein_id": "XP_047291539.1",
          "transcript_support_level": null,
          "aa_start": 322,
          "aa_end": null,
          "aa_length": 372,
          "cds_start": 965,
          "cds_end": null,
          "cds_length": 1119,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047435583.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.958+178T>G",
          "hgvs_p": null,
          "transcript": "ENST00000924711.1",
          "protein_id": "ENSP00000594770.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 508,
          "cds_start": null,
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          "cds_length": 1527,
          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
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          "canonical": false,
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          ],
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          "intron_rank": 4,
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          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.919+178T>G",
          "hgvs_p": null,
          "transcript": "NM_001256124.2",
          "protein_id": "NP_001243053.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 495,
          "cds_start": null,
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          "cds_length": 1488,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001256124.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.910+178T>G",
          "hgvs_p": null,
          "transcript": "ENST00000946122.1",
          "protein_id": "ENSP00000616181.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 492,
          "cds_start": null,
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          "cds_length": 1479,
          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000946122.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.823+178T>G",
          "hgvs_p": null,
          "transcript": "ENST00000946121.1",
          "protein_id": "ENSP00000616180.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 463,
          "cds_start": null,
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        },
        {
          "aa_ref": null,
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          "strand": false,
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          ],
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          "exon_count": 5,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.538+178T>G",
          "hgvs_p": null,
          "transcript": "ENST00000543309.5",
          "protein_id": "ENSP00000441480.1",
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          "cds_start": null,
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          "cdna_start": null,
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          "biotype": "protein_coding",
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        },
        {
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
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          ],
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          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.717+434T>G",
          "hgvs_p": null,
          "transcript": "ENST00000540128.1",
          "protein_id": "ENSP00000444607.1",
          "transcript_support_level": 3,
          "aa_start": null,
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          "aa_length": 243,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000540128.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "TRIM65",
          "gene_hgnc_id": 27316,
          "hgvs_c": "c.139+178T>G",
          "hgvs_p": null,
          "transcript": "ENST00000592642.1",
          "protein_id": "ENSP00000466353.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 90,
          "cds_start": null,
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          "cds_length": 273,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000592642.1"
        },
        {
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          "biotype": "pseudogene",
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      ],
      "gene_symbol": "TRIM65",
      "gene_hgnc_id": 27316,
      "dbsnp": "rs758468327",
      "frequency_reference_population": 0.0000043412197,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 7,
      "gnomad_exomes_af": 0.00000410882,
      "gnomad_genomes_af": 0.00000657125,
      "gnomad_exomes_ac": 6,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.1670660376548767,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.18000000715255737,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.575,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.0827,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.01,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 1.745,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.18,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_173547.4",
          "gene_symbol": "TRIM65",
          "hgnc_id": 27316,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.965T>G",
          "hgvs_p": "p.Leu322Arg"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}