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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-77482288-G-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=77482288&ref=G&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "17",
      "pos": 77482288,
      "ref": "G",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "ENST00000427177.6",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEPTIN9",
          "gene_hgnc_id": 7323,
          "hgvs_c": "c.866G>T",
          "hgvs_p": "p.Arg289Leu",
          "transcript": "NM_001113491.2",
          "protein_id": "NP_001106963.1",
          "transcript_support_level": null,
          "aa_start": 289,
          "aa_end": null,
          "aa_length": 586,
          "cds_start": 866,
          "cds_end": null,
          "cds_length": 1761,
          "cdna_start": 903,
          "cdna_end": null,
          "cdna_length": 3733,
          "mane_select": "ENST00000427177.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEPTIN9",
          "gene_hgnc_id": 7323,
          "hgvs_c": "c.866G>T",
          "hgvs_p": "p.Arg289Leu",
          "transcript": "ENST00000427177.6",
          "protein_id": "ENSP00000391249.1",
          "transcript_support_level": 1,
          "aa_start": 289,
          "aa_end": null,
          "aa_length": 586,
          "cds_start": 866,
          "cds_end": null,
          "cds_length": 1761,
          "cdna_start": 903,
          "cdna_end": null,
          "cdna_length": 3733,
          "mane_select": "NM_001113491.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEPTIN9",
          "gene_hgnc_id": 7323,
          "hgvs_c": "c.812G>T",
          "hgvs_p": "p.Arg271Leu",
          "transcript": "NM_006640.5",
          "protein_id": "NP_006631.2",
          "transcript_support_level": null,
          "aa_start": 271,
          "aa_end": null,
          "aa_length": 568,
          "cds_start": 812,
          "cds_end": null,
          "cds_length": 1707,
          "cdna_start": 1621,
          "cdna_end": null,
          "cdna_length": 4451,
          "mane_select": null,
          "mane_plus": "ENST00000329047.13",
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEPTIN9",
          "gene_hgnc_id": 7323,
          "hgvs_c": "c.812G>T",
          "hgvs_p": "p.Arg271Leu",
          "transcript": "ENST00000329047.13",
          "protein_id": "ENSP00000329161.8",
          "transcript_support_level": 1,
          "aa_start": 271,
          "aa_end": null,
          "aa_length": 568,
          "cds_start": 812,
          "cds_end": null,
          "cds_length": 1707,
          "cdna_start": 1621,
          "cdna_end": null,
          "cdna_length": 4451,
          "mane_select": null,
          "mane_plus": "NM_006640.5",
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEPTIN9",
          "gene_hgnc_id": 7323,
          "hgvs_c": "c.845G>T",
          "hgvs_p": "p.Arg282Leu",
          "transcript": "ENST00000423034.6",
          "protein_id": "ENSP00000405877.1",
          "transcript_support_level": 1,
          "aa_start": 282,
          "aa_end": null,
          "aa_length": 579,
          "cds_start": 845,
          "cds_end": null,
          "cds_length": 1740,
          "cdna_start": 1153,
          "cdna_end": null,
          "cdna_length": 3984,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEPTIN9",
          "gene_hgnc_id": 7323,
          "hgvs_c": "c.374G>T",
          "hgvs_p": "p.Arg125Leu",
          "transcript": "ENST00000427674.6",
          "protein_id": "ENSP00000403194.1",
          "transcript_support_level": 1,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 422,
          "cds_start": 374,
          "cds_end": null,
          "cds_length": 1269,
          "cdna_start": 1106,
          "cdna_end": null,
          "cdna_length": 3937,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEPTIN9",
          "gene_hgnc_id": 7323,
          "hgvs_c": "c.113G>T",
          "hgvs_p": "p.Arg38Leu",
          "transcript": "ENST00000541152.6",
          "protein_id": "ENSP00000438089.2",
          "transcript_support_level": 1,
          "aa_start": 38,
          "aa_end": null,
          "aa_length": 335,
          "cds_start": 113,
          "cds_end": null,
          "cds_length": 1008,
          "cdna_start": 195,
          "cdna_end": null,
          "cdna_length": 3026,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEPTIN9",
          "gene_hgnc_id": 7323,
          "hgvs_c": "c.845G>T",
          "hgvs_p": "p.Arg282Leu",
          "transcript": "NM_001113493.2",
          "protein_id": "NP_001106965.1",
          "transcript_support_level": null,
          "aa_start": 282,
          "aa_end": null,
          "aa_length": 579,
          "cds_start": 845,
          "cds_end": null,
          "cds_length": 1740,
          "cdna_start": 1006,
          "cdna_end": null,
          "cdna_length": 3836,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEPTIN9",
          "gene_hgnc_id": 7323,
          "hgvs_c": "c.809G>T",
          "hgvs_p": "p.Arg270Leu",
          "transcript": "NM_001293695.2",
          "protein_id": "NP_001280624.1",
          "transcript_support_level": null,
          "aa_start": 270,
          "aa_end": null,
          "aa_length": 567,
          "cds_start": 809,
          "cds_end": null,
          "cds_length": 1704,
          "cdna_start": 846,
          "cdna_end": null,
          "cdna_length": 3676,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "R",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SEPTIN9",
          "gene_hgnc_id": 7323,
          "hgvs_c": "c.809G>T",
          "hgvs_p": "p.Arg270Leu",
          "transcript": "ENST00000591198.5",
          "protein_id": "ENSP00000468406.1",
          "transcript_support_level": 2,
          "aa_start": 270,
          "aa_end": null,
          "aa_length": 567,
          "cds_start": 809,
          "cds_end": null,
          "cds_length": 1704,
          "cdna_start": 894,
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          "mane_select": null,
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        {
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          "strand": true,
          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "SEPTIN9",
          "gene_hgnc_id": 7323,
          "hgvs_c": "c.374G>T",
          "hgvs_p": "p.Arg125Leu",
          "transcript": "NM_001113492.2",
          "protein_id": "NP_001106964.1",
          "transcript_support_level": null,
          "aa_start": 125,
          "aa_end": null,
          "aa_length": 422,
          "cds_start": 374,
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          "cds_length": 1269,
          "cdna_start": 1123,
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          "mane_select": null,
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          "biotype": null,
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        {
          "aa_ref": "R",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
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          "exon_count": 11,
          "intron_rank": null,
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          "gene_symbol": "SEPTIN9",
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          "hgvs_c": "c.374G>T",
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        {
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          "exon_rank": 4,
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          "gene_symbol": "SEPTIN9",
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          "hgvs_c": "c.374G>T",
          "hgvs_p": "p.Arg125Leu",
          "transcript": "ENST00000431235.6",
          "protein_id": "ENSP00000406987.2",
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        {
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          "intron_rank": null,
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          "gene_symbol": "SEPTIN9",
          "gene_hgnc_id": 7323,
          "hgvs_c": "c.374G>T",
          "hgvs_p": "p.Arg125Leu",
          "transcript": "ENST00000449803.6",
          "protein_id": "ENSP00000400181.2",
          "transcript_support_level": 2,
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        {
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        {
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          "intron_rank": null,
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          "gene_symbol": "SEPTIN9",
          "gene_hgnc_id": 7323,
          "hgvs_c": "c.194G>T",
          "hgvs_p": "p.Arg65Leu",
          "transcript": "NM_001293696.2",
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        {
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          "hgvs_c": "c.194G>T",
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        {
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          "gene_symbol": "SEPTIN9",
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        {
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        {
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          "intron_rank": null,
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          "gene_symbol": "SEPTIN9",
          "gene_hgnc_id": 7323,
          "hgvs_c": "c.113G>T",
          "hgvs_p": "p.Arg38Leu",
          "transcript": "NM_001293697.2",
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          "cdna_length": 3020,
          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
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            "missense_variant"
          ],
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          "intron_rank": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "SEPTIN9",
          "gene_hgnc_id": 7323,
          "hgvs_c": "n.196-5194G>T",
          "hgvs_p": null,
          "transcript": "ENST00000588575.1",
          "protein_id": "ENSP00000468090.1",
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 559,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "SEPTIN9",
      "gene_hgnc_id": 7323,
      "dbsnp": "rs587781247",
      "frequency_reference_population": 6.845039e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84504e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8115385174751282,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.513,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.9837,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.13,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 6.831,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
          "pathogenic_score": 3,
          "criteria": [
            "PM2",
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000427177.6",
          "gene_symbol": "SEPTIN9",
          "hgnc_id": 7323,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.866G>T",
          "hgvs_p": "p.Arg289Leu"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}