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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-78204840-A-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=78204840&ref=A&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "17",
      "pos": 78204840,
      "ref": "A",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_001145526.3",
      "consequences": [
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.407A>G",
          "hgvs_p": "p.His136Arg",
          "transcript": "NM_001010982.5",
          "protein_id": "NP_001010982.2",
          "transcript_support_level": null,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 303,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 912,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000409257.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001010982.5"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.407A>G",
          "hgvs_p": "p.His136Arg",
          "transcript": "ENST00000409257.10",
          "protein_id": "ENSP00000386890.4",
          "transcript_support_level": 1,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 303,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 912,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001010982.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000409257.10"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.407A>G",
          "hgvs_p": "p.His136Arg",
          "transcript": "ENST00000327898.9",
          "protein_id": "ENSP00000328938.5",
          "transcript_support_level": 1,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 308,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 927,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000327898.9"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.500A>G",
          "hgvs_p": "p.His167Arg",
          "transcript": "ENST00000857474.1",
          "protein_id": "ENSP00000527533.1",
          "transcript_support_level": null,
          "aa_start": 167,
          "aa_end": null,
          "aa_length": 334,
          "cds_start": 500,
          "cds_end": null,
          "cds_length": 1005,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857474.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.494A>G",
          "hgvs_p": "p.His165Arg",
          "transcript": "ENST00000857484.1",
          "protein_id": "ENSP00000527543.1",
          "transcript_support_level": null,
          "aa_start": 165,
          "aa_end": null,
          "aa_length": 332,
          "cds_start": 494,
          "cds_end": null,
          "cds_length": 999,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857484.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.407A>G",
          "hgvs_p": "p.His136Arg",
          "transcript": "ENST00000965675.1",
          "protein_id": "ENSP00000635734.1",
          "transcript_support_level": null,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 327,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 984,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000965675.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.407A>G",
          "hgvs_p": "p.His136Arg",
          "transcript": "ENST00000857463.1",
          "protein_id": "ENSP00000527522.1",
          "transcript_support_level": null,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 322,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 969,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857463.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.458A>G",
          "hgvs_p": "p.His153Arg",
          "transcript": "ENST00000857469.1",
          "protein_id": "ENSP00000527528.1",
          "transcript_support_level": null,
          "aa_start": 153,
          "aa_end": null,
          "aa_length": 320,
          "cds_start": 458,
          "cds_end": null,
          "cds_length": 963,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857469.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.425A>G",
          "hgvs_p": "p.His142Arg",
          "transcript": "ENST00000857477.1",
          "protein_id": "ENSP00000527536.1",
          "transcript_support_level": null,
          "aa_start": 142,
          "aa_end": null,
          "aa_length": 314,
          "cds_start": 425,
          "cds_end": null,
          "cds_length": 945,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857477.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.410A>G",
          "hgvs_p": "p.His137Arg",
          "transcript": "ENST00000857470.1",
          "protein_id": "ENSP00000527529.1",
          "transcript_support_level": null,
          "aa_start": 137,
          "aa_end": null,
          "aa_length": 309,
          "cds_start": 410,
          "cds_end": null,
          "cds_length": 930,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857470.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.407A>G",
          "hgvs_p": "p.His136Arg",
          "transcript": "NM_001145526.3",
          "protein_id": "NP_001138998.1",
          "transcript_support_level": null,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 308,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 927,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001145526.3"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.413A>G",
          "hgvs_p": "p.His138Arg",
          "transcript": "ENST00000857478.1",
          "protein_id": "ENSP00000527537.1",
          "transcript_support_level": null,
          "aa_start": 138,
          "aa_end": null,
          "aa_length": 305,
          "cds_start": 413,
          "cds_end": null,
          "cds_length": 918,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857478.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.410A>G",
          "hgvs_p": "p.His137Arg",
          "transcript": "ENST00000857464.1",
          "protein_id": "ENSP00000527523.1",
          "transcript_support_level": null,
          "aa_start": 137,
          "aa_end": null,
          "aa_length": 304,
          "cds_start": 410,
          "cds_end": null,
          "cds_length": 915,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857464.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.302A>G",
          "hgvs_p": "p.His101Arg",
          "transcript": "ENST00000857480.1",
          "protein_id": "ENSP00000527539.1",
          "transcript_support_level": null,
          "aa_start": 101,
          "aa_end": null,
          "aa_length": 273,
          "cds_start": 302,
          "cds_end": null,
          "cds_length": 822,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857480.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.407A>G",
          "hgvs_p": "p.His136Arg",
          "transcript": "ENST00000857481.1",
          "protein_id": "ENSP00000527540.1",
          "transcript_support_level": null,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 273,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 822,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857481.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.302A>G",
          "hgvs_p": "p.His101Arg",
          "transcript": "ENST00000857465.1",
          "protein_id": "ENSP00000527524.1",
          "transcript_support_level": null,
          "aa_start": 101,
          "aa_end": null,
          "aa_length": 268,
          "cds_start": 302,
          "cds_end": null,
          "cds_length": 807,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000857465.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.230A>G",
          "hgvs_p": "p.His77Arg",
          "transcript": "ENST00000857482.1",
          "protein_id": "ENSP00000527541.1",
          "transcript_support_level": null,
          "aa_start": 77,
          "aa_end": null,
          "aa_length": 249,
          "cds_start": 230,
          "cds_end": null,
          "cds_length": 750,
          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.230A>G",
          "hgvs_p": "p.His77Arg",
          "transcript": "ENST00000857476.1",
          "protein_id": "ENSP00000527535.1",
          "transcript_support_level": null,
          "aa_start": 77,
          "aa_end": null,
          "aa_length": 244,
          "cds_start": 230,
          "cds_end": null,
          "cds_length": 735,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857476.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.407A>G",
          "hgvs_p": "p.His136Arg",
          "transcript": "NM_001391999.1",
          "protein_id": "NP_001378928.1",
          "transcript_support_level": null,
          "aa_start": 136,
          "aa_end": null,
          "aa_length": 240,
          "cds_start": 407,
          "cds_end": null,
          "cds_length": 723,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001391999.1"
        },
        {
          "aa_ref": "H",
          "aa_alt": "R",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "AFMID",
          "gene_hgnc_id": 20910,
          "hgvs_c": "c.167A>G",
          "hgvs_p": "p.His56Arg",
          "transcript": "ENST00000857485.1",
          "protein_id": "ENSP00000527544.1",
          "transcript_support_level": null,
          "aa_start": 56,
          "aa_end": null,
          "aa_length": 223,
          "cds_start": 167,
          "cds_end": null,
          "cds_length": 672,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000857485.1"
        },
        {
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        {
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        {
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          ],
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        {
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          ],
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          "gene_symbol": "AFMID",
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          "hgvs_c": "n.309-600A>G",
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          "transcript": "ENST00000588199.5",
          "protein_id": "ENSP00000465406.1",
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          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000588199.5"
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      ],
      "gene_symbol": "AFMID",
      "gene_hgnc_id": 20910,
      "dbsnp": "rs764562456",
      "frequency_reference_population": 0.00002540068,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 41,
      "gnomad_exomes_af": 0.0000205214,
      "gnomad_genomes_af": 0.0000722553,
      "gnomad_exomes_ac": 30,
      "gnomad_genomes_ac": 11,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.05284148454666138,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.05999999865889549,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.064,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0603,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.74,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.987,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.06,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001145526.3",
          "gene_symbol": "AFMID",
          "hgnc_id": 20910,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.407A>G",
          "hgvs_p": "p.His136Arg"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}