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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 17-8377625-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=17&pos=8377625&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "17",
      "pos": 8377625,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_000987.5",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "NM_000987.5",
          "protein_id": "NP_000978.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000648839.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000987.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "ENST00000648839.1",
          "protein_id": "ENSP00000498177.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000987.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000648839.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 3,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000263809",
          "gene_hgnc_id": null,
          "hgvs_c": "n.310-1521G>A",
          "hgvs_p": null,
          "transcript": "ENST00000582471.1",
          "protein_id": "ENSP00000463847.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000582471.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.401G>A",
          "hgvs_p": "p.Arg134His",
          "transcript": "ENST00000913691.1",
          "protein_id": "ENSP00000583750.1",
          "transcript_support_level": null,
          "aa_start": 134,
          "aa_end": null,
          "aa_length": 153,
          "cds_start": 401,
          "cds_end": null,
          "cds_length": 462,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000913691.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.401G>A",
          "hgvs_p": "p.Arg134His",
          "transcript": "ENST00000913696.1",
          "protein_id": "ENSP00000583755.1",
          "transcript_support_level": null,
          "aa_start": 134,
          "aa_end": null,
          "aa_length": 153,
          "cds_start": 401,
          "cds_end": null,
          "cds_length": 462,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000913696.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.383G>A",
          "hgvs_p": "p.Arg128His",
          "transcript": "ENST00000913688.1",
          "protein_id": "ENSP00000583747.1",
          "transcript_support_level": null,
          "aa_start": 128,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": 383,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000913688.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "NM_001315530.2",
          "protein_id": "NP_001302459.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001315530.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "NM_001315531.2",
          "protein_id": "NP_001302460.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001315531.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "ENST00000582556.5",
          "protein_id": "ENSP00000463470.1",
          "transcript_support_level": 2,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000582556.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "ENST00000583011.6",
          "protein_id": "ENSP00000462322.1",
          "transcript_support_level": 3,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000583011.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "ENST00000584164.6",
          "protein_id": "ENSP00000463784.1",
          "transcript_support_level": 2,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000584164.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "ENST00000851389.1",
          "protein_id": "ENSP00000521448.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851389.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "ENST00000851390.1",
          "protein_id": "ENSP00000521449.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851390.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "ENST00000851392.1",
          "protein_id": "ENSP00000521451.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851392.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "ENST00000851394.1",
          "protein_id": "ENSP00000521453.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851394.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "ENST00000913690.1",
          "protein_id": "ENSP00000583749.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000913690.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "ENST00000913692.1",
          "protein_id": "ENSP00000583751.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000913692.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "ENST00000913697.1",
          "protein_id": "ENSP00000583756.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000913697.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "ENST00000913699.1",
          "protein_id": "ENSP00000583758.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000913699.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL26",
          "gene_hgnc_id": 10327,
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His",
          "transcript": "ENST00000913702.1",
          "protein_id": "ENSP00000583761.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 145,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 438,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000913702.1"
        },
        {
          "aa_ref": "R",
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      "dbsnp": "rs932068728",
      "frequency_reference_population": 0.000004118588,
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      "allele_count_reference_population": 6,
      "gnomad_exomes_af": 0.00000411859,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 6,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.3348715305328369,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.266,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.4928,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.18,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 6.056,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4,BS2",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 5,
          "pathogenic_score": 0,
          "criteria": [
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            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_000987.5",
          "gene_symbol": "RPL26",
          "hgnc_id": 10327,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.377G>A",
          "hgvs_p": "p.Arg126His"
        },
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000582471.1",
          "gene_symbol": "ENSG00000263809",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.310-1521G>A",
          "hgvs_p": null
        },
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000579904.6",
          "gene_symbol": "ENSG00000265749",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.543+1514C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Diamond-Blackfan anemia,Diamond-Blackfan anemia 11,not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:4",
      "phenotype_combined": "Diamond-Blackfan anemia|not specified|Diamond-Blackfan anemia 11",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}