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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 18-21384325-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=18&pos=21384325&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "18",
      "pos": 21384325,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001410867.1",
      "consequences": [
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "NM_001142966.3",
          "protein_id": "NP_001136438.1",
          "transcript_support_level": null,
          "aa_start": 93,
          "aa_end": null,
          "aa_length": 1923,
          "cds_start": 277,
          "cds_end": null,
          "cds_length": 5772,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000424526.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001142966.3"
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "ENST00000424526.7",
          "protein_id": "ENSP00000412060.1",
          "transcript_support_level": 5,
          "aa_start": 93,
          "aa_end": null,
          "aa_length": 1923,
          "cds_start": 277,
          "cds_end": null,
          "cds_length": 5772,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001142966.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000424526.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "n.382G>A",
          "hgvs_p": null,
          "transcript": "ENST00000578368.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000578368.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "n.548G>A",
          "hgvs_p": null,
          "transcript": "ENST00000584446.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000584446.5"
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "ENST00000861016.1",
          "protein_id": "ENSP00000531075.1",
          "transcript_support_level": null,
          "aa_start": 93,
          "aa_end": null,
          "aa_length": 1967,
          "cds_start": 277,
          "cds_end": null,
          "cds_length": 5904,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000861016.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "NM_001410867.1",
          "protein_id": "NP_001397796.1",
          "transcript_support_level": null,
          "aa_start": 93,
          "aa_end": null,
          "aa_length": 1966,
          "cds_start": 277,
          "cds_end": null,
          "cds_length": 5901,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001410867.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "ENST00000579454.2",
          "protein_id": "ENSP00000463926.2",
          "transcript_support_level": 5,
          "aa_start": 93,
          "aa_end": null,
          "aa_length": 1966,
          "cds_start": 277,
          "cds_end": null,
          "cds_length": 5901,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000579454.2"
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "ENST00000580732.6",
          "protein_id": "ENSP00000464162.1",
          "transcript_support_level": 5,
          "aa_start": 93,
          "aa_end": null,
          "aa_length": 1923,
          "cds_start": 277,
          "cds_end": null,
          "cds_length": 5772,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000580732.6"
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 33,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "ENST00000942000.1",
          "protein_id": "ENSP00000612059.1",
          "transcript_support_level": null,
          "aa_start": 93,
          "aa_end": null,
          "aa_length": 1899,
          "cds_start": 277,
          "cds_end": null,
          "cds_length": 5700,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942000.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "NM_001410868.1",
          "protein_id": "NP_001397797.1",
          "transcript_support_level": null,
          "aa_start": 93,
          "aa_end": null,
          "aa_length": 1814,
          "cds_start": 277,
          "cds_end": null,
          "cds_length": 5445,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001410868.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "ENST00000269218.10",
          "protein_id": "ENSP00000269218.6",
          "transcript_support_level": 5,
          "aa_start": 93,
          "aa_end": null,
          "aa_length": 1814,
          "cds_start": 277,
          "cds_end": null,
          "cds_length": 5445,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000269218.10"
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "XM_017025989.2",
          "protein_id": "XP_016881478.1",
          "transcript_support_level": null,
          "aa_start": 93,
          "aa_end": null,
          "aa_length": 1998,
          "cds_start": 277,
          "cds_end": null,
          "cds_length": 5997,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017025989.2"
        },
        {
          "aa_ref": "E",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "XM_047437809.1",
          "protein_id": "XP_047293765.1",
          "transcript_support_level": null,
          "aa_start": 93,
          "aa_end": null,
          "aa_length": 1998,
          "cds_start": 277,
          "cds_end": null,
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          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047437809.1"
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "XM_047437810.1",
          "protein_id": "XP_047293766.1",
          "transcript_support_level": null,
          "aa_start": 93,
          "aa_end": null,
          "aa_length": 1998,
          "cds_start": 277,
          "cds_end": null,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "E",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
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          "intron_rank": null,
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          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "XM_017025990.2",
          "protein_id": "XP_016881479.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 1997,
          "cds_start": 277,
          "cds_end": null,
          "cds_length": 5994,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_017025990.2"
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "XM_017025991.2",
          "protein_id": "XP_016881480.1",
          "transcript_support_level": null,
          "aa_start": 93,
          "aa_end": null,
          "aa_length": 1970,
          "cds_start": 277,
          "cds_end": null,
          "cds_length": 5913,
          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "XM_017025991.2"
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 32,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "XM_047437811.1",
          "protein_id": "XP_047293767.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 1969,
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          "cdna_start": null,
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        },
        {
          "aa_ref": "E",
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          "protein_coding": true,
          "strand": true,
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          ],
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          "exon_count": 34,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "XM_006722547.4",
          "protein_id": "XP_006722610.1",
          "transcript_support_level": null,
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          "cds_start": 277,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "E",
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          "protein_coding": true,
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          ],
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          "exon_count": 30,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "XM_017025993.2",
          "protein_id": "XP_016881482.1",
          "transcript_support_level": null,
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          "aa_end": null,
          "aa_length": 1955,
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          "cdna_start": null,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "XM_017025993.2"
        },
        {
          "aa_ref": "E",
          "aa_alt": "K",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GREB1L",
          "gene_hgnc_id": 31042,
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys",
          "transcript": "XM_047437813.1",
          "protein_id": "XP_047293769.1",
          "transcript_support_level": null,
          "aa_start": 93,
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          "aa_length": 1955,
          "cds_start": 277,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
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          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_187982.1"
        }
      ],
      "gene_symbol": "GREB1L",
      "gene_hgnc_id": 31042,
      "dbsnp": "rs185578147",
      "frequency_reference_population": 0.0041533913,
      "hom_count_reference_population": 19,
      "allele_count_reference_population": 6445,
      "gnomad_exomes_af": 0.00427033,
      "gnomad_genomes_af": 0.00307904,
      "gnomad_exomes_ac": 5976,
      "gnomad_genomes_ac": 469,
      "gnomad_exomes_homalt": 17,
      "gnomad_genomes_homalt": 2,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0039663612842559814,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.202,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.3103,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.35,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 9.138,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -13,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -13,
          "benign_score": 13,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_001410867.1",
          "gene_symbol": "GREB1L",
          "hgnc_id": 31042,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.277G>A",
          "hgvs_p": "p.Glu93Lys"
        },
        {
          "score": -9,
          "benign_score": 9,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000584611.2",
          "gene_symbol": "GREB1L-AS1",
          "hgnc_id": 58310,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.319+3188C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "GREB1L-related disorder,Mayer-Rokitansky-Kuster-Hauser syndrome,Renal hypodysplasia/aplasia 3,not provided",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:2 B:2",
      "phenotype_combined": "Renal hypodysplasia/aplasia 3;Mayer-Rokitansky-Kuster-Hauser syndrome|not provided|GREB1L-related disorder",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}