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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 18-70005258-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=18&pos=70005258&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "18",
      "pos": 70005258,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_173630.4",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 48,
          "exon_rank_end": null,
          "exon_count": 49,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.6535G>C",
          "hgvs_p": "p.Ala2179Pro",
          "transcript": "NM_173630.4",
          "protein_id": "NP_775901.3",
          "transcript_support_level": null,
          "aa_start": 2179,
          "aa_end": null,
          "aa_length": 2226,
          "cds_start": 6535,
          "cds_end": null,
          "cds_length": 6681,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000640769.2",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_173630.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 48,
          "exon_rank_end": null,
          "exon_count": 49,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.6535G>C",
          "hgvs_p": "p.Ala2179Pro",
          "transcript": "ENST00000640769.2",
          "protein_id": "ENSP00000491507.1",
          "transcript_support_level": 2,
          "aa_start": 2179,
          "aa_end": null,
          "aa_length": 2226,
          "cds_start": 6535,
          "cds_end": null,
          "cds_length": 6681,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_173630.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000640769.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 47,
          "exon_rank_end": null,
          "exon_count": 48,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "n.*4849G>C",
          "hgvs_p": null,
          "transcript": "ENST00000581161.5",
          "protein_id": "ENSP00000462926.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000581161.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 42,
          "exon_rank_end": null,
          "exon_count": 43,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "n.*3775G>C",
          "hgvs_p": null,
          "transcript": "ENST00000583043.5",
          "protein_id": "ENSP00000462733.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000583043.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 47,
          "exon_rank_end": null,
          "exon_count": 48,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "n.*4849G>C",
          "hgvs_p": null,
          "transcript": "ENST00000581161.5",
          "protein_id": "ENSP00000462926.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000581161.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 42,
          "exon_rank_end": null,
          "exon_count": 43,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "n.*3775G>C",
          "hgvs_p": null,
          "transcript": "ENST00000583043.5",
          "protein_id": "ENSP00000462733.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000583043.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 48,
          "exon_rank_end": null,
          "exon_count": 49,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.6556G>C",
          "hgvs_p": "p.Ala2186Pro",
          "transcript": "ENST00000917940.1",
          "protein_id": "ENSP00000587999.1",
          "transcript_support_level": null,
          "aa_start": 2186,
          "aa_end": null,
          "aa_length": 2233,
          "cds_start": 6556,
          "cds_end": null,
          "cds_length": 6702,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917940.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 48,
          "exon_rank_end": null,
          "exon_count": 49,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.6538G>C",
          "hgvs_p": "p.Ala2180Pro",
          "transcript": "ENST00000917941.1",
          "protein_id": "ENSP00000588000.1",
          "transcript_support_level": null,
          "aa_start": 2180,
          "aa_end": null,
          "aa_length": 2227,
          "cds_start": 6538,
          "cds_end": null,
          "cds_length": 6684,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917941.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 48,
          "exon_rank_end": null,
          "exon_count": 49,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.6535G>C",
          "hgvs_p": "p.Ala2179Pro",
          "transcript": "ENST00000255674.11",
          "protein_id": "ENSP00000255674.7",
          "transcript_support_level": 5,
          "aa_start": 2179,
          "aa_end": null,
          "aa_length": 2200,
          "cds_start": 6535,
          "cds_end": null,
          "cds_length": 6603,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000255674.11"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 30,
          "exon_rank_end": null,
          "exon_count": 31,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.3934G>C",
          "hgvs_p": "p.Ala1312Pro",
          "transcript": "ENST00000677824.1",
          "protein_id": "ENSP00000504646.1",
          "transcript_support_level": null,
          "aa_start": 1312,
          "aa_end": null,
          "aa_length": 1359,
          "cds_start": 3934,
          "cds_end": null,
          "cds_length": 4080,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000677824.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 47,
          "exon_rank_end": null,
          "exon_count": 48,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.3799G>C",
          "hgvs_p": "p.Ala1267Pro",
          "transcript": "NM_001318520.2",
          "protein_id": "NP_001305449.1",
          "transcript_support_level": null,
          "aa_start": 1267,
          "aa_end": null,
          "aa_length": 1314,
          "cds_start": 3799,
          "cds_end": null,
          "cds_length": 3945,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001318520.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.1042G>C",
          "hgvs_p": "p.Ala348Pro",
          "transcript": "ENST00000578780.2",
          "protein_id": "ENSP00000462675.2",
          "transcript_support_level": 5,
          "aa_start": 348,
          "aa_end": null,
          "aa_length": 395,
          "cds_start": 1042,
          "cds_end": null,
          "cds_length": 1188,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000578780.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 48,
          "exon_rank_end": null,
          "exon_count": 49,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.6538G>C",
          "hgvs_p": "p.Ala2180Pro",
          "transcript": "XM_006722434.4",
          "protein_id": "XP_006722497.1",
          "transcript_support_level": null,
          "aa_start": 2180,
          "aa_end": null,
          "aa_length": 2227,
          "cds_start": 6538,
          "cds_end": null,
          "cds_length": 6684,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_006722434.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 47,
          "exon_rank_end": null,
          "exon_count": 48,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.6298G>C",
          "hgvs_p": "p.Ala2100Pro",
          "transcript": "XM_011525902.3",
          "protein_id": "XP_011524204.1",
          "transcript_support_level": null,
          "aa_start": 2100,
          "aa_end": null,
          "aa_length": 2147,
          "cds_start": 6298,
          "cds_end": null,
          "cds_length": 6444,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011525902.3"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 47,
          "exon_rank_end": null,
          "exon_count": 48,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.6295G>C",
          "hgvs_p": "p.Ala2099Pro",
          "transcript": "XM_017025693.2",
          "protein_id": "XP_016881182.1",
          "transcript_support_level": null,
          "aa_start": 2099,
          "aa_end": null,
          "aa_length": 2146,
          "cds_start": 6295,
          "cds_end": null,
          "cds_length": 6441,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017025693.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 46,
          "exon_rank_end": null,
          "exon_count": 47,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.6109G>C",
          "hgvs_p": "p.Ala2037Pro",
          "transcript": "XM_011525903.3",
          "protein_id": "XP_011524205.1",
          "transcript_support_level": null,
          "aa_start": 2037,
          "aa_end": null,
          "aa_length": 2084,
          "cds_start": 6109,
          "cds_end": null,
          "cds_length": 6255,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011525903.3"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 46,
          "exon_rank_end": null,
          "exon_count": 47,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.6106G>C",
          "hgvs_p": "p.Ala2036Pro",
          "transcript": "XM_047437468.1",
          "protein_id": "XP_047293424.1",
          "transcript_support_level": null,
          "aa_start": 2036,
          "aa_end": null,
          "aa_length": 2083,
          "cds_start": 6106,
          "cds_end": null,
          "cds_length": 6252,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047437468.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 45,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.5896G>C",
          "hgvs_p": "p.Ala1966Pro",
          "transcript": "XM_017025694.2",
          "protein_id": "XP_016881183.1",
          "transcript_support_level": null,
          "aa_start": 1966,
          "aa_end": null,
          "aa_length": 2013,
          "cds_start": 5896,
          "cds_end": null,
          "cds_length": 6042,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017025694.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 41,
          "exon_rank_end": null,
          "exon_count": 42,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.5473G>C",
          "hgvs_p": "p.Ala1825Pro",
          "transcript": "XM_017025695.2",
          "protein_id": "XP_016881184.1",
          "transcript_support_level": null,
          "aa_start": 1825,
          "aa_end": null,
          "aa_length": 1872,
          "cds_start": 5473,
          "cds_end": null,
          "cds_length": 5619,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017025695.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 38,
          "exon_rank_end": null,
          "exon_count": 39,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.5038G>C",
          "hgvs_p": "p.Ala1680Pro",
          "transcript": "XM_011525906.3",
          "protein_id": "XP_011524208.1",
          "transcript_support_level": null,
          "aa_start": 1680,
          "aa_end": null,
          "aa_length": 1727,
          "cds_start": 5038,
          "cds_end": null,
          "cds_length": 5184,
          "cdna_start": null,
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        {
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          "feature": "ENST00000679113.1"
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      ],
      "gene_symbol": "RTTN",
      "gene_hgnc_id": 18654,
      "dbsnp": "rs587780444",
      "frequency_reference_population": 0.000027397973,
      "hom_count_reference_population": 1,
      "allele_count_reference_population": 40,
      "gnomad_exomes_af": 0.000027398,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 40,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 1,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.0939355194568634,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.228,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.3563,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.19,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.662,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -3,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate,BS1_Supporting",
      "acmg_by_gene": [
        {
          "score": -3,
          "benign_score": 3,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BS1_Supporting"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_173630.4",
          "gene_symbol": "RTTN",
          "hgnc_id": 18654,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.6535G>C",
          "hgvs_p": "p.Ala2179Pro"
        }
      ],
      "clinvar_disease": "not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:2",
      "phenotype_combined": "not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}