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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 18-70127695-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=18&pos=70127695&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "18",
      "pos": 70127695,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000640769.2",
      "consequences": [
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 49,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.3190A>C",
          "hgvs_p": "p.Lys1064Gln",
          "transcript": "NM_173630.4",
          "protein_id": "NP_775901.3",
          "transcript_support_level": null,
          "aa_start": 1064,
          "aa_end": null,
          "aa_length": 2226,
          "cds_start": 3190,
          "cds_end": null,
          "cds_length": 6681,
          "cdna_start": 3219,
          "cdna_end": null,
          "cdna_length": 7830,
          "mane_select": "ENST00000640769.2",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 49,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.3190A>C",
          "hgvs_p": "p.Lys1064Gln",
          "transcript": "ENST00000640769.2",
          "protein_id": "ENSP00000491507.1",
          "transcript_support_level": 2,
          "aa_start": 1064,
          "aa_end": null,
          "aa_length": 2226,
          "cds_start": 3190,
          "cds_end": null,
          "cds_length": 6681,
          "cdna_start": 3219,
          "cdna_end": null,
          "cdna_length": 7830,
          "mane_select": "NM_173630.4",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 48,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "n.*1504A>C",
          "hgvs_p": null,
          "transcript": "ENST00000581161.5",
          "protein_id": "ENSP00000462926.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6722,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 43,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "n.*461A>C",
          "hgvs_p": null,
          "transcript": "ENST00000583043.5",
          "protein_id": "ENSP00000462733.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6278,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 48,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "n.*1504A>C",
          "hgvs_p": null,
          "transcript": "ENST00000581161.5",
          "protein_id": "ENSP00000462926.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6722,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 43,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "n.*461A>C",
          "hgvs_p": null,
          "transcript": "ENST00000583043.5",
          "protein_id": "ENSP00000462733.1",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 6278,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 49,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.3190A>C",
          "hgvs_p": "p.Lys1064Gln",
          "transcript": "ENST00000255674.11",
          "protein_id": "ENSP00000255674.7",
          "transcript_support_level": 5,
          "aa_start": 1064,
          "aa_end": null,
          "aa_length": 2200,
          "cds_start": 3190,
          "cds_end": null,
          "cds_length": 6603,
          "cdna_start": 3190,
          "cdna_end": null,
          "cdna_length": 6960,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 24,
          "exon_rank_end": null,
          "exon_count": 48,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.454A>C",
          "hgvs_p": "p.Lys152Gln",
          "transcript": "NM_001318520.2",
          "protein_id": "NP_001305449.1",
          "transcript_support_level": null,
          "aa_start": 152,
          "aa_end": null,
          "aa_length": 1314,
          "cds_start": 454,
          "cds_end": null,
          "cds_length": 3945,
          "cdna_start": 3036,
          "cdna_end": null,
          "cdna_length": 7647,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.178A>C",
          "hgvs_p": "p.Lys60Gln",
          "transcript": "ENST00000638298.1",
          "protein_id": "ENSP00000492180.1",
          "transcript_support_level": 5,
          "aa_start": 60,
          "aa_end": null,
          "aa_length": 296,
          "cds_start": 178,
          "cds_end": null,
          "cds_length": 891,
          "cdna_start": 179,
          "cdna_end": null,
          "cdna_length": 892,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 49,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.3193A>C",
          "hgvs_p": "p.Lys1065Gln",
          "transcript": "XM_006722434.4",
          "protein_id": "XP_006722497.1",
          "transcript_support_level": null,
          "aa_start": 1065,
          "aa_end": null,
          "aa_length": 2227,
          "cds_start": 3193,
          "cds_end": null,
          "cds_length": 6684,
          "cdna_start": 3222,
          "cdna_end": null,
          "cdna_length": 7833,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
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          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.3193A>C",
          "hgvs_p": "p.Lys1065Gln",
          "transcript": "XM_006722435.4",
          "protein_id": "XP_006722498.1",
          "transcript_support_level": null,
          "aa_start": 1065,
          "aa_end": null,
          "aa_length": 2059,
          "cds_start": 3193,
          "cds_end": null,
          "cds_length": 6180,
          "cdna_start": 3222,
          "cdna_end": null,
          "cdna_length": 8425,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 22,
          "exon_rank_end": null,
          "exon_count": 46,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.2551A>C",
          "hgvs_p": "p.Lys851Gln",
          "transcript": "XM_017025694.2",
          "protein_id": "XP_016881183.1",
          "transcript_support_level": null,
          "aa_start": 851,
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          "aa_length": 2013,
          "cds_start": 2551,
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          "cds_length": 6042,
          "cdna_start": 2816,
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          "cdna_length": 7427,
          "mane_select": null,
          "mane_plus": null,
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        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 45,
          "intron_rank": null,
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          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.3193A>C",
          "hgvs_p": "p.Lys1065Gln",
          "transcript": "XM_011525904.4",
          "protein_id": "XP_011524206.1",
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          "aa_length": 1990,
          "cds_start": 3193,
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          "cdna_start": 3222,
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          "mane_select": null,
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          "feature": null
        },
        {
          "aa_ref": "K",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 42,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.2128A>C",
          "hgvs_p": "p.Lys710Gln",
          "transcript": "XM_017025695.2",
          "protein_id": "XP_016881184.1",
          "transcript_support_level": null,
          "aa_start": 710,
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          "aa_length": 1872,
          "cds_start": 2128,
          "cds_end": null,
          "cds_length": 5619,
          "cdna_start": 2254,
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          "mane_select": null,
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        },
        {
          "aa_ref": "K",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.3193A>C",
          "hgvs_p": "p.Lys1065Gln",
          "transcript": "XM_011525905.3",
          "protein_id": "XP_011524207.1",
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          "feature": null
        },
        {
          "aa_ref": "K",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 39,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.1693A>C",
          "hgvs_p": "p.Lys565Gln",
          "transcript": "XM_011525906.3",
          "protein_id": "XP_011524208.1",
          "transcript_support_level": null,
          "aa_start": 565,
          "aa_end": null,
          "aa_length": 1727,
          "cds_start": 1693,
          "cds_end": null,
          "cds_length": 5184,
          "cdna_start": 1947,
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          "mane_select": null,
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          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "K",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 36,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.3193A>C",
          "hgvs_p": "p.Lys1065Gln",
          "transcript": "XM_011525907.3",
          "protein_id": "XP_011524209.1",
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          "aa_length": 1597,
          "cds_start": 3193,
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          "cds_length": 4794,
          "cdna_start": 3222,
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          "feature": null
        },
        {
          "aa_ref": "K",
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          "strand": false,
          "consequences": [
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          ],
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          "intron_rank": null,
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          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.3190A>C",
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          "transcript": "XM_047437469.1",
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          "feature": null
        },
        {
          "aa_ref": "K",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_rank_end": null,
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          "intron_rank": null,
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          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "c.3193A>C",
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          "transcript": "XM_047437470.1",
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          "cdna_length": 3780,
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          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 25,
          "exon_rank_end": null,
          "exon_count": 35,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RTTN",
          "gene_hgnc_id": 18654,
          "hgvs_c": "n.*1182A>C",
          "hgvs_p": null,
          "transcript": "ENST00000638251.1",
          "protein_id": "ENSP00000491968.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4801,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
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          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 29,
          "intron_rank": null,
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      ],
      "gene_symbol": "RTTN",
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      "dbsnp": "rs864321621",
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
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      "gnomad_genomes_ac": null,
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      "gnomad_mito_homoplasmic": null,
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      "computational_score_selected": 0.21609234809875488,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.12999999523162842,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.073,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1207,
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      "bayesdelnoaf_score": -0.55,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.849,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.13,
      "spliceai_max_prediction": "Benign",
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      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP5_Moderate,BP4",
      "acmg_by_gene": [
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          "benign_score": 1,
          "pathogenic_score": 4,
          "criteria": [
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            "BP4"
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          "verdict": "Uncertain_significance",
          "transcript": "ENST00000640769.2",
          "gene_symbol": "RTTN",
          "hgnc_id": 18654,
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          "hgvs_p": "p.Lys1064Gln"
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      ],
      "clinvar_disease": "Microcephalic primordial dwarfism due to RTTN deficiency,Primary microcephaly",
      "clinvar_classification": "Likely pathogenic",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LP:1",
      "phenotype_combined": "Primary microcephaly|Microcephalic primordial dwarfism due to RTTN deficiency",
      "pathogenicity_classification_combined": "Likely pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}