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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-12940316-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=12940316&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 12940316,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_004343.4",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "c.566G>C",
          "hgvs_p": "p.Ser189Thr",
          "transcript": "NM_004343.4",
          "protein_id": "NP_004334.1",
          "transcript_support_level": null,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 417,
          "cds_start": 566,
          "cds_end": null,
          "cds_length": 1254,
          "cdna_start": 637,
          "cdna_end": null,
          "cdna_length": 1901,
          "mane_select": "ENST00000316448.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004343.4"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "c.566G>C",
          "hgvs_p": "p.Ser189Thr",
          "transcript": "ENST00000316448.10",
          "protein_id": "ENSP00000320866.4",
          "transcript_support_level": 1,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 417,
          "cds_start": 566,
          "cds_end": null,
          "cds_length": 1254,
          "cdna_start": 637,
          "cdna_end": null,
          "cdna_length": 1901,
          "mane_select": "NM_004343.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000316448.10"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "c.566G>C",
          "hgvs_p": "p.Ser189Thr",
          "transcript": "ENST00000957023.1",
          "protein_id": "ENSP00000627082.1",
          "transcript_support_level": null,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 415,
          "cds_start": 566,
          "cds_end": null,
          "cds_length": 1248,
          "cdna_start": 637,
          "cdna_end": null,
          "cdna_length": 1895,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000957023.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "c.566G>C",
          "hgvs_p": "p.Ser189Thr",
          "transcript": "ENST00000929790.1",
          "protein_id": "ENSP00000599849.1",
          "transcript_support_level": null,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 413,
          "cds_start": 566,
          "cds_end": null,
          "cds_length": 1242,
          "cdna_start": 637,
          "cdna_end": null,
          "cdna_length": 1889,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000929790.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "c.566G>C",
          "hgvs_p": "p.Ser189Thr",
          "transcript": "ENST00000929788.1",
          "protein_id": "ENSP00000599847.1",
          "transcript_support_level": null,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 410,
          "cds_start": 566,
          "cds_end": null,
          "cds_length": 1233,
          "cdna_start": 637,
          "cdna_end": null,
          "cdna_length": 1880,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000929788.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "c.566G>C",
          "hgvs_p": "p.Ser189Thr",
          "transcript": "ENST00000929786.1",
          "protein_id": "ENSP00000599845.1",
          "transcript_support_level": null,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 387,
          "cds_start": 566,
          "cds_end": null,
          "cds_length": 1164,
          "cdna_start": 637,
          "cdna_end": null,
          "cdna_length": 1812,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000929786.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "c.566G>C",
          "hgvs_p": "p.Ser189Thr",
          "transcript": "ENST00000586760.2",
          "protein_id": "ENSP00000465918.2",
          "transcript_support_level": 2,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 378,
          "cds_start": 566,
          "cds_end": null,
          "cds_length": 1137,
          "cdna_start": 637,
          "cdna_end": null,
          "cdna_length": 1630,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000586760.2"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "c.362G>C",
          "hgvs_p": "p.Ser121Thr",
          "transcript": "ENST00000588454.6",
          "protein_id": "ENSP00000465105.2",
          "transcript_support_level": 3,
          "aa_start": 121,
          "aa_end": null,
          "aa_length": 349,
          "cds_start": 362,
          "cds_end": null,
          "cds_length": 1050,
          "cdna_start": 397,
          "cdna_end": null,
          "cdna_length": 1661,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000588454.6"
        },
        {
          "aa_ref": "S",
          "aa_alt": "T",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "c.566G>C",
          "hgvs_p": "p.Ser189Thr",
          "transcript": "ENST00000929789.1",
          "protein_id": "ENSP00000599848.1",
          "transcript_support_level": null,
          "aa_start": 189,
          "aa_end": null,
          "aa_length": 311,
          "cds_start": 566,
          "cds_end": null,
          "cds_length": 936,
          "cdna_start": 637,
          "cdna_end": null,
          "cdna_length": 1583,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000929789.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 4,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "c.430-56G>C",
          "hgvs_p": null,
          "transcript": "ENST00000929787.1",
          "protein_id": "ENSP00000599846.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1709,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000929787.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "c.549+17G>C",
          "hgvs_p": null,
          "transcript": "ENST00000929792.1",
          "protein_id": "ENSP00000599851.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 264,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 795,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1442,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000929792.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "c.549+17G>C",
          "hgvs_p": null,
          "transcript": "ENST00000929791.1",
          "protein_id": "ENSP00000599850.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 231,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 696,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1343,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000929791.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "c.45+1592G>C",
          "hgvs_p": null,
          "transcript": "ENST00000861560.1",
          "protein_id": "ENSP00000531619.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 112,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 339,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 986,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000861560.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "n.566G>C",
          "hgvs_p": null,
          "transcript": "ENST00000586967.2",
          "protein_id": "ENSP00000466037.2",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1678,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000586967.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "n.727G>C",
          "hgvs_p": null,
          "transcript": "ENST00000590325.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 781,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000590325.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "n.566G>C",
          "hgvs_p": null,
          "transcript": "ENST00000680816.1",
          "protein_id": "ENSP00000504963.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1564,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000680816.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MIR6515",
          "gene_hgnc_id": 50227,
          "hgvs_c": "n.-168G>C",
          "hgvs_p": null,
          "transcript": "ENST00000619843.1",
          "protein_id": null,
          "transcript_support_level": 6,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 57,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "miRNA",
          "feature": "ENST00000619843.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MIR6515",
          "gene_hgnc_id": 50227,
          "hgvs_c": "n.-168G>C",
          "hgvs_p": null,
          "transcript": "NR_106770.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 57,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "NR_106770.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": null,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MIR6515",
          "gene_hgnc_id": 50227,
          "hgvs_c": "n.-170G>C",
          "hgvs_p": null,
          "transcript": "unassigned_transcript_3232",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 21,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "miRNA",
          "feature": "unassigned_transcript_3232"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": null,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 1,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MIR6515",
          "gene_hgnc_id": 50227,
          "hgvs_c": "n.-205G>C",
          "hgvs_p": null,
          "transcript": "unassigned_transcript_3233",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 20,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "miRNA",
          "feature": "unassigned_transcript_3233"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CALR",
          "gene_hgnc_id": 1455,
          "hgvs_c": "n.*169G>C",
          "hgvs_p": null,
          "transcript": "ENST00000587486.6",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 957,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000587486.6"
        }
      ],
      "gene_symbol": "CALR",
      "gene_hgnc_id": 1455,
      "dbsnp": "rs147369833",
      "frequency_reference_population": 0.001509103,
      "hom_count_reference_population": 1,
      "allele_count_reference_population": 2436,
      "gnomad_exomes_af": 0.00157673,
      "gnomad_genomes_af": 0.000860043,
      "gnomad_exomes_ac": 2305,
      "gnomad_genomes_ac": 131,
      "gnomad_exomes_homalt": 1,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.022202253341674805,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.029999999329447746,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.036,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.0567,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.49,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 1.637,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.03,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -9,
      "acmg_classification": "Benign",
      "acmg_criteria": "BP4_Strong,BP6,BS2",
      "acmg_by_gene": [
        {
          "score": -9,
          "benign_score": 9,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "NM_004343.4",
          "gene_symbol": "CALR",
          "hgnc_id": 1455,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.566G>C",
          "hgvs_p": "p.Ser189Thr"
        },
        {
          "score": -5,
          "benign_score": 5,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Strong",
            "BP6"
          ],
          "verdict": "Likely_benign",
          "transcript": "NR_106770.1",
          "gene_symbol": "MIR6515",
          "hgnc_id": 50227,
          "effects": [
            "upstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.-168G>C",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "CALR-related disorder",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "no assertion criteria provided",
      "clinvar_submissions_summary": "null",
      "phenotype_combined": "CALR-related disorder",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}
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