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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-17555726-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=17555726&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 17555726,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_024656.4",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COLGALT1",
          "gene_hgnc_id": 26182,
          "hgvs_c": "c.13C>T",
          "hgvs_p": "p.Pro5Ser",
          "transcript": "NM_024656.4",
          "protein_id": "NP_078932.2",
          "transcript_support_level": null,
          "aa_start": 5,
          "aa_end": null,
          "aa_length": 622,
          "cds_start": 13,
          "cds_end": null,
          "cds_length": 1869,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000252599.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_024656.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COLGALT1",
          "gene_hgnc_id": 26182,
          "hgvs_c": "c.13C>T",
          "hgvs_p": "p.Pro5Ser",
          "transcript": "ENST00000252599.9",
          "protein_id": "ENSP00000252599.3",
          "transcript_support_level": 1,
          "aa_start": 5,
          "aa_end": null,
          "aa_length": 622,
          "cds_start": 13,
          "cds_end": null,
          "cds_length": 1869,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_024656.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000252599.9"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COLGALT1",
          "gene_hgnc_id": 26182,
          "hgvs_c": "c.13C>T",
          "hgvs_p": "p.Pro5Ser",
          "transcript": "ENST00000886053.1",
          "protein_id": "ENSP00000556112.1",
          "transcript_support_level": null,
          "aa_start": 5,
          "aa_end": null,
          "aa_length": 688,
          "cds_start": 13,
          "cds_end": null,
          "cds_length": 2067,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000886053.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COLGALT1",
          "gene_hgnc_id": 26182,
          "hgvs_c": "c.13C>T",
          "hgvs_p": "p.Pro5Ser",
          "transcript": "ENST00000886054.1",
          "protein_id": "ENSP00000556113.1",
          "transcript_support_level": null,
          "aa_start": 5,
          "aa_end": null,
          "aa_length": 680,
          "cds_start": 13,
          "cds_end": null,
          "cds_length": 2043,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000886054.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COLGALT1",
          "gene_hgnc_id": 26182,
          "hgvs_c": "c.13C>T",
          "hgvs_p": "p.Pro5Ser",
          "transcript": "ENST00000886055.1",
          "protein_id": "ENSP00000556114.1",
          "transcript_support_level": null,
          "aa_start": 5,
          "aa_end": null,
          "aa_length": 650,
          "cds_start": 13,
          "cds_end": null,
          "cds_length": 1953,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000886055.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COLGALT1",
          "gene_hgnc_id": 26182,
          "hgvs_c": "c.13C>T",
          "hgvs_p": "p.Pro5Ser",
          "transcript": "ENST00000970586.1",
          "protein_id": "ENSP00000640645.1",
          "transcript_support_level": null,
          "aa_start": 5,
          "aa_end": null,
          "aa_length": 631,
          "cds_start": 13,
          "cds_end": null,
          "cds_length": 1896,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970586.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COLGALT1",
          "gene_hgnc_id": 26182,
          "hgvs_c": "c.13C>T",
          "hgvs_p": "p.Pro5Ser",
          "transcript": "ENST00000912419.1",
          "protein_id": "ENSP00000582478.1",
          "transcript_support_level": null,
          "aa_start": 5,
          "aa_end": null,
          "aa_length": 585,
          "cds_start": 13,
          "cds_end": null,
          "cds_length": 1758,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912419.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COLGALT1",
          "gene_hgnc_id": 26182,
          "hgvs_c": "c.13C>T",
          "hgvs_p": "p.Pro5Ser",
          "transcript": "ENST00000597147.2",
          "protein_id": "ENSP00000468947.2",
          "transcript_support_level": 5,
          "aa_start": 5,
          "aa_end": null,
          "aa_length": 459,
          "cds_start": 13,
          "cds_end": null,
          "cds_length": 1382,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000597147.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COLGALT1",
          "gene_hgnc_id": 26182,
          "hgvs_c": "n.13C>T",
          "hgvs_p": null,
          "transcript": "ENST00000690106.1",
          "protein_id": "ENSP00000508762.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000690106.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COLGALT1",
          "gene_hgnc_id": 26182,
          "hgvs_c": "n.13C>T",
          "hgvs_p": null,
          "transcript": "ENST00000690841.1",
          "protein_id": "ENSP00000509464.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000690841.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COLGALT1",
          "gene_hgnc_id": 26182,
          "hgvs_c": "n.13C>T",
          "hgvs_p": null,
          "transcript": "ENST00000690947.1",
          "protein_id": "ENSP00000510367.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000690947.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COLGALT1",
          "gene_hgnc_id": 26182,
          "hgvs_c": "c.-810C>T",
          "hgvs_p": null,
          "transcript": "XM_005260080.5",
          "protein_id": "XP_005260137.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 539,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1620,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_005260080.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "NIBAN3",
          "gene_hgnc_id": 24130,
          "hgvs_c": "c.*567C>T",
          "hgvs_p": null,
          "transcript": "XM_011527786.3",
          "protein_id": "XP_011526088.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 591,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1776,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011527786.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "upstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "COLGALT1",
          "gene_hgnc_id": 26182,
          "hgvs_c": "n.-75C>T",
          "hgvs_p": null,
          "transcript": "ENST00000692764.1",
          "protein_id": null,
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000692764.1"
        }
      ],
      "gene_symbol": "COLGALT1",
      "gene_hgnc_id": 26182,
      "dbsnp": "rs1012740258",
      "frequency_reference_population": 0.00003257503,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 39,
      "gnomad_exomes_af": 0.000018169,
      "gnomad_genomes_af": 0.000132013,
      "gnomad_exomes_ac": 19,
      "gnomad_genomes_ac": 20,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.07512128353118896,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.351,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.0901,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.19,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.164,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -3,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate,BS1_Supporting",
      "acmg_by_gene": [
        {
          "score": -3,
          "benign_score": 3,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate",
            "BS1_Supporting"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_024656.4",
          "gene_symbol": "COLGALT1",
          "hgnc_id": 26182,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.13C>T",
          "hgvs_p": "p.Pro5Ser"
        },
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "XM_011527786.3",
          "gene_symbol": "NIBAN3",
          "hgnc_id": 24130,
          "effects": [
            "downstream_gene_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.*567C>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Inborn genetic diseases",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}