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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-17861359-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=17861359&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 17861359,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_000980.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.85C>T",
          "hgvs_p": "p.Arg29Cys",
          "transcript": "NM_000980.4",
          "protein_id": "NP_000971.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 176,
          "cds_start": 85,
          "cds_end": null,
          "cds_length": 531,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000222247.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_000980.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.85C>T",
          "hgvs_p": "p.Arg29Cys",
          "transcript": "ENST00000222247.10",
          "protein_id": "ENSP00000222247.4",
          "transcript_support_level": 1,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 176,
          "cds_start": 85,
          "cds_end": null,
          "cds_length": 531,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_000980.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000222247.10"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.-3C>T",
          "hgvs_p": null,
          "transcript": "ENST00000599870.1",
          "protein_id": "ENSP00000471748.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000599870.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.-3C>T",
          "hgvs_p": null,
          "transcript": "ENST00000597648.5",
          "protein_id": "ENSP00000473155.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 140,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 424,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000597648.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.85C>T",
          "hgvs_p": "p.Arg29Cys",
          "transcript": "ENST00000916998.1",
          "protein_id": "ENSP00000587057.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 174,
          "cds_start": 85,
          "cds_end": null,
          "cds_length": 525,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916998.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.85C>T",
          "hgvs_p": "p.Arg29Cys",
          "transcript": "ENST00000917001.1",
          "protein_id": "ENSP00000587060.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 173,
          "cds_start": 85,
          "cds_end": null,
          "cds_length": 522,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917001.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.85C>T",
          "hgvs_p": "p.Arg29Cys",
          "transcript": "ENST00000917002.1",
          "protein_id": "ENSP00000587061.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 171,
          "cds_start": 85,
          "cds_end": null,
          "cds_length": 516,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917002.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.85C>T",
          "hgvs_p": "p.Arg29Cys",
          "transcript": "ENST00000917005.1",
          "protein_id": "ENSP00000587064.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 168,
          "cds_start": 85,
          "cds_end": null,
          "cds_length": 507,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917005.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.85C>T",
          "hgvs_p": "p.Arg29Cys",
          "transcript": "ENST00000917003.1",
          "protein_id": "ENSP00000587062.1",
          "transcript_support_level": null,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 166,
          "cds_start": 85,
          "cds_end": null,
          "cds_length": 501,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917003.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "C",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.85C>T",
          "hgvs_p": "p.Arg29Cys",
          "transcript": "ENST00000600147.5",
          "protein_id": "ENSP00000471584.1",
          "transcript_support_level": 5,
          "aa_start": 29,
          "aa_end": null,
          "aa_length": 154,
          "cds_start": 85,
          "cds_end": null,
          "cds_length": 465,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000600147.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.-3C>T",
          "hgvs_p": null,
          "transcript": "ENST00000599870.1",
          "protein_id": "ENSP00000471748.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 147,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 444,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000599870.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.-3C>T",
          "hgvs_p": null,
          "transcript": "ENST00000597648.5",
          "protein_id": "ENSP00000473155.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 140,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 424,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000597648.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.33+52C>T",
          "hgvs_p": null,
          "transcript": "ENST00000599898.5",
          "protein_id": "ENSP00000471360.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 137,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 414,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000599898.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.19-66C>T",
          "hgvs_p": null,
          "transcript": "ENST00000916999.1",
          "protein_id": "ENSP00000587058.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 132,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 399,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000916999.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.19-735C>T",
          "hgvs_p": null,
          "transcript": "ENST00000878958.1",
          "protein_id": "ENSP00000549017.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 116,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 351,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000878958.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.7-747C>T",
          "hgvs_p": null,
          "transcript": "ENST00000917006.1",
          "protein_id": "ENSP00000587065.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 108,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 327,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917006.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.7-765C>T",
          "hgvs_p": null,
          "transcript": "ENST00000917000.1",
          "protein_id": "ENSP00000587059.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 102,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 309,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917000.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "c.6+1397C>T",
          "hgvs_p": null,
          "transcript": "ENST00000917004.1",
          "protein_id": "ENSP00000587063.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 50,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 153,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000917004.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "n.27C>T",
          "hgvs_p": null,
          "transcript": "ENST00000600238.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000600238.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPL18A",
          "gene_hgnc_id": 10311,
          "hgvs_c": "n.89C>T",
          "hgvs_p": null,
          "transcript": "ENST00000602216.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000602216.1"
        }
      ],
      "gene_symbol": "RPL18A",
      "gene_hgnc_id": 10311,
      "dbsnp": "rs369237728",
      "frequency_reference_population": 0.00008183854,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 132,
      "gnomad_exomes_af": 0.0000855731,
      "gnomad_genomes_af": 0.0000459945,
      "gnomad_exomes_ac": 125,
      "gnomad_genomes_ac": 7,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8627800941467285,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.436,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.9049,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.26,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.642,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PP3_Moderate,BS2",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PP3_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_000980.4",
          "gene_symbol": "RPL18A",
          "hgnc_id": 10311,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.85C>T",
          "hgvs_p": "p.Arg29Cys"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}