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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-35125237-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=35125237&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 35125237,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_139284.3",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LGI4",
          "gene_hgnc_id": 18712,
          "hgvs_c": "c.1570C>T",
          "hgvs_p": "p.Pro524Ser",
          "transcript": "NM_139284.3",
          "protein_id": "NP_644813.1",
          "transcript_support_level": null,
          "aa_start": 524,
          "aa_end": null,
          "aa_length": 537,
          "cds_start": 1570,
          "cds_end": null,
          "cds_length": 1614,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000310123.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_139284.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LGI4",
          "gene_hgnc_id": 18712,
          "hgvs_c": "c.1570C>T",
          "hgvs_p": "p.Pro524Ser",
          "transcript": "ENST00000310123.8",
          "protein_id": "ENSP00000312273.3",
          "transcript_support_level": 1,
          "aa_start": 524,
          "aa_end": null,
          "aa_length": 537,
          "cds_start": 1570,
          "cds_end": null,
          "cds_length": 1614,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_139284.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000310123.8"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LGI4",
          "gene_hgnc_id": 18712,
          "hgvs_c": "c.*931C>T",
          "hgvs_p": null,
          "transcript": "ENST00000587780.5",
          "protein_id": "ENSP00000467044.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 377,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1134,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000587780.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LGI4",
          "gene_hgnc_id": 18712,
          "hgvs_c": "n.1629C>T",
          "hgvs_p": null,
          "transcript": "ENST00000493050.5",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000493050.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LGI4",
          "gene_hgnc_id": 18712,
          "hgvs_c": "c.1567C>T",
          "hgvs_p": "p.Pro523Ser",
          "transcript": "ENST00000874489.1",
          "protein_id": "ENSP00000544548.1",
          "transcript_support_level": null,
          "aa_start": 523,
          "aa_end": null,
          "aa_length": 536,
          "cds_start": 1567,
          "cds_end": null,
          "cds_length": 1611,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874489.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LGI4",
          "gene_hgnc_id": 18712,
          "hgvs_c": "c.1531C>T",
          "hgvs_p": "p.Pro511Ser",
          "transcript": "ENST00000874487.1",
          "protein_id": "ENSP00000544546.1",
          "transcript_support_level": null,
          "aa_start": 511,
          "aa_end": null,
          "aa_length": 524,
          "cds_start": 1531,
          "cds_end": null,
          "cds_length": 1575,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874487.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LGI4",
          "gene_hgnc_id": 18712,
          "hgvs_c": "c.1498C>T",
          "hgvs_p": "p.Pro500Ser",
          "transcript": "ENST00000874488.1",
          "protein_id": "ENSP00000544547.1",
          "transcript_support_level": null,
          "aa_start": 500,
          "aa_end": null,
          "aa_length": 513,
          "cds_start": 1498,
          "cds_end": null,
          "cds_length": 1542,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874488.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LGI4",
          "gene_hgnc_id": 18712,
          "hgvs_c": "c.1054C>T",
          "hgvs_p": "p.Pro352Ser",
          "transcript": "XM_017026428.1",
          "protein_id": "XP_016881917.1",
          "transcript_support_level": null,
          "aa_start": 352,
          "aa_end": null,
          "aa_length": 365,
          "cds_start": 1054,
          "cds_end": null,
          "cds_length": 1098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017026428.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LGI4",
          "gene_hgnc_id": 18712,
          "hgvs_c": "c.1054C>T",
          "hgvs_p": "p.Pro352Ser",
          "transcript": "XM_017026429.1",
          "protein_id": "XP_016881918.1",
          "transcript_support_level": null,
          "aa_start": 352,
          "aa_end": null,
          "aa_length": 365,
          "cds_start": 1054,
          "cds_end": null,
          "cds_length": 1098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017026429.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LGI4",
          "gene_hgnc_id": 18712,
          "hgvs_c": "c.1054C>T",
          "hgvs_p": "p.Pro352Ser",
          "transcript": "XM_017026430.2",
          "protein_id": "XP_016881919.1",
          "transcript_support_level": null,
          "aa_start": 352,
          "aa_end": null,
          "aa_length": 365,
          "cds_start": 1054,
          "cds_end": null,
          "cds_length": 1098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_017026430.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LGI4",
          "gene_hgnc_id": 18712,
          "hgvs_c": "c.1054C>T",
          "hgvs_p": "p.Pro352Ser",
          "transcript": "XM_047438343.1",
          "protein_id": "XP_047294299.1",
          "transcript_support_level": null,
          "aa_start": 352,
          "aa_end": null,
          "aa_length": 365,
          "cds_start": 1054,
          "cds_end": null,
          "cds_length": 1098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047438343.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "LGI4",
          "gene_hgnc_id": 18712,
          "hgvs_c": "c.*162C>T",
          "hgvs_p": null,
          "transcript": "ENST00000392225.7",
          "protein_id": "ENSP00000376059.3",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 494,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1485,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000392225.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FXYD3",
          "gene_hgnc_id": 4027,
          "hgvs_c": "c.*1780G>A",
          "hgvs_p": null,
          "transcript": "ENST00000874694.1",
          "protein_id": "ENSP00000544753.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 87,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 264,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874694.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 10,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FXYD3",
          "gene_hgnc_id": 4027,
          "hgvs_c": "c.*1780G>A",
          "hgvs_p": null,
          "transcript": "ENST00000874695.1",
          "protein_id": "ENSP00000544754.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 87,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 264,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874695.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FXYD3",
          "gene_hgnc_id": 4027,
          "hgvs_c": "c.*1780G>A",
          "hgvs_p": null,
          "transcript": "ENST00000874696.1",
          "protein_id": "ENSP00000544755.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 87,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 264,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000874696.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": 5,
          "intron_rank_end": null,
          "gene_symbol": "LGI4",
          "gene_hgnc_id": 18712,
          "hgvs_c": "n.420-371C>T",
          "hgvs_p": null,
          "transcript": "ENST00000591840.5",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000591840.5"
        }
      ],
      "gene_symbol": "LGI4",
      "gene_hgnc_id": 18712,
      "dbsnp": "rs368479590",
      "frequency_reference_population": 0.000001403046,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 0.00000140305,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.2038583755493164,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.072,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.125,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.29,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.359,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_139284.3",
          "gene_symbol": "LGI4",
          "hgnc_id": 18712,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1570C>T",
          "hgvs_p": "p.Pro524Ser"
        },
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000874694.1",
          "gene_symbol": "FXYD3",
          "hgnc_id": 4027,
          "effects": [
            "3_prime_UTR_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.*1780G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Inborn genetic diseases",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}