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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-36003450-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=36003450&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 36003450,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "ENST00000324444.9",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "c.1102G>C",
          "hgvs_p": "p.Val368Leu",
          "transcript": "NM_001039876.3",
          "protein_id": "NP_001034965.1",
          "transcript_support_level": null,
          "aa_start": 368,
          "aa_end": null,
          "aa_length": 404,
          "cds_start": 1102,
          "cds_end": null,
          "cds_length": 1215,
          "cdna_start": 1234,
          "cdna_end": null,
          "cdna_length": 1377,
          "mane_select": "ENST00000324444.9",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "c.1102G>C",
          "hgvs_p": "p.Val368Leu",
          "transcript": "ENST00000324444.9",
          "protein_id": "ENSP00000316130.3",
          "transcript_support_level": 5,
          "aa_start": 368,
          "aa_end": null,
          "aa_length": 404,
          "cds_start": 1102,
          "cds_end": null,
          "cds_length": 1215,
          "cdna_start": 1234,
          "cdna_end": null,
          "cdna_length": 1377,
          "mane_select": "NM_001039876.3",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "c.763G>C",
          "hgvs_p": "p.Val255Leu",
          "transcript": "ENST00000340477.9",
          "protein_id": "ENSP00000343152.5",
          "transcript_support_level": 1,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 291,
          "cds_start": 763,
          "cds_end": null,
          "cds_length": 876,
          "cdna_start": 864,
          "cdna_end": null,
          "cdna_length": 1004,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "c.923G>C",
          "hgvs_p": "p.Gly308Ala",
          "transcript": "ENST00000490730.1",
          "protein_id": "ENSP00000422716.1",
          "transcript_support_level": 2,
          "aa_start": 308,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 923,
          "cds_end": null,
          "cds_length": 1063,
          "cdna_start": 1035,
          "cdna_end": null,
          "cdna_length": 1175,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "c.763G>C",
          "hgvs_p": "p.Val255Leu",
          "transcript": "NM_001297735.3",
          "protein_id": "NP_001284664.1",
          "transcript_support_level": null,
          "aa_start": 255,
          "aa_end": null,
          "aa_length": 291,
          "cds_start": 763,
          "cds_end": null,
          "cds_length": 876,
          "cdna_start": 895,
          "cdna_end": null,
          "cdna_length": 1038,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "c.311G>C",
          "hgvs_p": "p.Gly104Ala",
          "transcript": "ENST00000503121.5",
          "protein_id": "ENSP00000425550.1",
          "transcript_support_level": 3,
          "aa_start": 104,
          "aa_end": null,
          "aa_length": 149,
          "cds_start": 311,
          "cds_end": null,
          "cds_length": 451,
          "cdna_start": 313,
          "cdna_end": null,
          "cdna_length": 453,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "c.958G>C",
          "hgvs_p": "p.Val320Leu",
          "transcript": "XM_047438344.1",
          "protein_id": "XP_047294300.1",
          "transcript_support_level": null,
          "aa_start": 320,
          "aa_end": null,
          "aa_length": 356,
          "cds_start": 958,
          "cds_end": null,
          "cds_length": 1071,
          "cdna_start": 1090,
          "cdna_end": null,
          "cdna_length": 1233,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "c.862G>C",
          "hgvs_p": "p.Val288Leu",
          "transcript": "XM_047438345.1",
          "protein_id": "XP_047294301.1",
          "transcript_support_level": null,
          "aa_start": 288,
          "aa_end": null,
          "aa_length": 324,
          "cds_start": 862,
          "cds_end": null,
          "cds_length": 975,
          "cdna_start": 1083,
          "cdna_end": null,
          "cdna_length": 1226,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "c.853G>C",
          "hgvs_p": "p.Val285Leu",
          "transcript": "XM_047438346.1",
          "protein_id": "XP_047294302.1",
          "transcript_support_level": null,
          "aa_start": 285,
          "aa_end": null,
          "aa_length": 321,
          "cds_start": 853,
          "cds_end": null,
          "cds_length": 966,
          "cdna_start": 985,
          "cdna_end": null,
          "cdna_length": 1128,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "c.718G>C",
          "hgvs_p": "p.Val240Leu",
          "transcript": "XM_047438347.1",
          "protein_id": "XP_047294303.1",
          "transcript_support_level": null,
          "aa_start": 240,
          "aa_end": null,
          "aa_length": 276,
          "cds_start": 718,
          "cds_end": null,
          "cds_length": 831,
          "cdna_start": 939,
          "cdna_end": null,
          "cdna_length": 1082,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "c.658G>C",
          "hgvs_p": "p.Val220Leu",
          "transcript": "XM_047438349.1",
          "protein_id": "XP_047294305.1",
          "transcript_support_level": null,
          "aa_start": 220,
          "aa_end": null,
          "aa_length": 256,
          "cds_start": 658,
          "cds_end": null,
          "cds_length": 771,
          "cdna_start": 790,
          "cdna_end": null,
          "cdna_length": 933,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "c.523G>C",
          "hgvs_p": "p.Val175Leu",
          "transcript": "XM_047438350.1",
          "protein_id": "XP_047294306.1",
          "transcript_support_level": null,
          "aa_start": 175,
          "aa_end": null,
          "aa_length": 211,
          "cds_start": 523,
          "cds_end": null,
          "cds_length": 636,
          "cdna_start": 744,
          "cdna_end": null,
          "cdna_length": 887,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "c.514G>C",
          "hgvs_p": "p.Val172Leu",
          "transcript": "XM_047438351.1",
          "protein_id": "XP_047294307.1",
          "transcript_support_level": null,
          "aa_start": 172,
          "aa_end": null,
          "aa_length": 208,
          "cds_start": 514,
          "cds_end": null,
          "cds_length": 627,
          "cdna_start": 646,
          "cdna_end": null,
          "cdna_length": 789,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "n.2199G>C",
          "hgvs_p": null,
          "transcript": "ENST00000465425.2",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2341,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "n.570G>C",
          "hgvs_p": null,
          "transcript": "ENST00000505054.2",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 713,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "c.*109G>C",
          "hgvs_p": null,
          "transcript": "ENST00000397428.8",
          "protein_id": "ENSP00000380572.4",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 43,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 132,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 382,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "SYNE4",
          "gene_hgnc_id": 26703,
          "hgvs_c": "c.*39G>C",
          "hgvs_p": null,
          "transcript": "XM_047438348.1",
          "protein_id": "XP_047294304.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 274,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 825,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1139,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "SYNE4",
      "gene_hgnc_id": 26703,
      "dbsnp": "rs141202530",
      "frequency_reference_population": 6.8450765e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84508e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.09298601746559143,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.009999999776482582,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.09,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1466,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.54,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.179,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000324444.9",
          "gene_symbol": "SYNE4",
          "hgnc_id": 26703,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.1102G>C",
          "hgvs_p": "p.Val368Leu"
        }
      ],
      "clinvar_disease": "",
      "clinvar_classification": "",
      "clinvar_review_status": "",
      "clinvar_submissions_summary": "",
      "phenotype_combined": null,
      "pathogenicity_classification_combined": null,
      "custom_annotations": null
    }
  ],
  "message": null
}