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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-39482885-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=39482885&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 39482885,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "ENST00000607714.6",
      "consequences": [
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "c.260G>C",
          "hgvs_p": "p.Gly87Ala",
          "transcript": "NM_001001563.5",
          "protein_id": "NP_001001563.2",
          "transcript_support_level": null,
          "aa_start": 87,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 260,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": 276,
          "cdna_end": null,
          "cdna_length": 5037,
          "mane_select": "ENST00000607714.6",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "c.260G>C",
          "hgvs_p": "p.Gly87Ala",
          "transcript": "ENST00000607714.6",
          "protein_id": "ENSP00000475531.1",
          "transcript_support_level": 1,
          "aa_start": 87,
          "aa_end": null,
          "aa_length": 353,
          "cds_start": 260,
          "cds_end": null,
          "cds_length": 1062,
          "cdna_start": 276,
          "cdna_end": null,
          "cdna_length": 5037,
          "mane_select": "NM_001001563.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "c.569G>C",
          "hgvs_p": "p.Gly190Ala",
          "transcript": "ENST00000544017.5",
          "protein_id": "ENSP00000445806.2",
          "transcript_support_level": 1,
          "aa_start": 190,
          "aa_end": null,
          "aa_length": 456,
          "cds_start": 569,
          "cds_end": null,
          "cds_length": 1371,
          "cdna_start": 702,
          "cdna_end": null,
          "cdna_length": 2572,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "n.260G>C",
          "hgvs_p": null,
          "transcript": "ENST00000601358.5",
          "protein_id": "ENSP00000472476.2",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 1481,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "c.257G>C",
          "hgvs_p": "p.Gly86Ala",
          "transcript": "ENST00000602028.5",
          "protein_id": "ENSP00000470896.1",
          "transcript_support_level": 5,
          "aa_start": 86,
          "aa_end": null,
          "aa_length": 255,
          "cds_start": 257,
          "cds_end": null,
          "cds_length": 769,
          "cdna_start": 258,
          "cdna_end": null,
          "cdna_length": 770,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "c.-21G>C",
          "hgvs_p": null,
          "transcript": "NM_001329559.2",
          "protein_id": "NP_001316488.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 240,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 723,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4978,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "c.242G>C",
          "hgvs_p": "p.Gly81Ala",
          "transcript": "ENST00000594583.2",
          "protein_id": "ENSP00000470994.1",
          "transcript_support_level": 3,
          "aa_start": 81,
          "aa_end": null,
          "aa_length": 225,
          "cds_start": 242,
          "cds_end": null,
          "cds_length": 678,
          "cdna_start": 244,
          "cdna_end": null,
          "cdna_length": 680,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "E",
          "aa_alt": "Q",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "c.109G>C",
          "hgvs_p": "p.Glu37Gln",
          "transcript": "ENST00000597666.5",
          "protein_id": "ENSP00000472723.2",
          "transcript_support_level": 3,
          "aa_start": 37,
          "aa_end": null,
          "aa_length": 199,
          "cds_start": 109,
          "cds_end": null,
          "cds_length": 600,
          "cdna_start": 113,
          "cdna_end": null,
          "cdna_length": 604,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "G",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "c.224G>C",
          "hgvs_p": "p.Gly75Ala",
          "transcript": "XM_011527491.4",
          "protein_id": "XP_011525793.1",
          "transcript_support_level": null,
          "aa_start": 75,
          "aa_end": null,
          "aa_length": 341,
          "cds_start": 224,
          "cds_end": null,
          "cds_length": 1026,
          "cdna_start": 258,
          "cdna_end": null,
          "cdna_length": 5019,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "splice_region_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "c.-113G>C",
          "hgvs_p": null,
          "transcript": "XM_047439681.1",
          "protein_id": "XP_047295637.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 229,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 690,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5000,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "n.256G>C",
          "hgvs_p": null,
          "transcript": "ENST00000595286.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 698,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "n.230G>C",
          "hgvs_p": null,
          "transcript": "ENST00000597782.5",
          "protein_id": "ENSP00000469052.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 456,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "n.268G>C",
          "hgvs_p": null,
          "transcript": "ENST00000598125.5",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 577,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "n.185G>C",
          "hgvs_p": null,
          "transcript": "ENST00000599733.5",
          "protein_id": "ENSP00000472904.1",
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 669,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "splice_region_variant",
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "n.185G>C",
          "hgvs_p": null,
          "transcript": "ENST00000602265.5",
          "protein_id": "ENSP00000472920.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 786,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "c.-21G>C",
          "hgvs_p": null,
          "transcript": "NM_001329559.2",
          "protein_id": "NP_001316488.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 240,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 723,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 4978,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "c.-113G>C",
          "hgvs_p": null,
          "transcript": "XM_047439681.1",
          "protein_id": "XP_047295637.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 229,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 690,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 5000,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": 2,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "c.259+852G>C",
          "hgvs_p": null,
          "transcript": "ENST00000601403.5",
          "protein_id": "ENSP00000472184.2",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 255,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 768,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 769,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "TIMM50",
          "gene_hgnc_id": 23656,
          "hgvs_c": "c.108+1924G>C",
          "hgvs_p": null,
          "transcript": "ENST00000599794.5",
          "protein_id": "ENSP00000472524.2",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 157,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 474,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 786,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        }
      ],
      "gene_symbol": "TIMM50",
      "gene_hgnc_id": 23656,
      "dbsnp": "rs776019250",
      "frequency_reference_population": 6.840591e-7,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 1,
      "gnomad_exomes_af": 6.84059e-7,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7140080332756042,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.9520000219345093,
      "splice_prediction_selected": "Pathogenic",
      "splice_source_selected": "dbscSNV1_RF",
      "revel_score": 0.415,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.81,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.09,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.925,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.04,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": 0.999241879645801,
      "dbscsnv_ada_prediction": "Pathogenic",
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 5,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3,PP5_Moderate",
      "acmg_by_gene": [
        {
          "score": 5,
          "benign_score": 0,
          "pathogenic_score": 5,
          "criteria": [
            "PM2",
            "PP3",
            "PP5_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000607714.6",
          "gene_symbol": "TIMM50",
          "hgnc_id": 23656,
          "effects": [
            "missense_variant",
            "splice_region_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.260G>C",
          "hgvs_p": "p.Gly87Ala"
        }
      ],
      "clinvar_disease": "3-methylglutaconic aciduria type 9,Mitochondrial encephalopathy",
      "clinvar_classification": "Pathogenic",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "P:1",
      "phenotype_combined": "Mitochondrial encephalopathy|3-methylglutaconic aciduria type 9",
      "pathogenicity_classification_combined": "Pathogenic",
      "custom_annotations": null
    }
  ],
  "message": null
}