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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-4099217-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=4099217&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 4099217,
      "ref": "G",
      "alt": "C",
      "effect": "synonymous_variant",
      "transcript": "NM_030662.4",
      "consequences": [
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.903C>G",
          "hgvs_p": "p.Pro301Pro",
          "transcript": "NM_030662.4",
          "protein_id": "NP_109587.1",
          "transcript_support_level": null,
          "aa_start": 301,
          "aa_end": null,
          "aa_length": 400,
          "cds_start": 903,
          "cds_end": null,
          "cds_length": 1203,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000262948.10",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_030662.4"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.903C>G",
          "hgvs_p": "p.Pro301Pro",
          "transcript": "ENST00000262948.10",
          "protein_id": "ENSP00000262948.4",
          "transcript_support_level": 1,
          "aa_start": 301,
          "aa_end": null,
          "aa_length": 400,
          "cds_start": 903,
          "cds_end": null,
          "cds_length": 1203,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_030662.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000262948.10"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.903C>G",
          "hgvs_p": "p.Pro301Pro",
          "transcript": "ENST00000945862.1",
          "protein_id": "ENSP00000615921.1",
          "transcript_support_level": null,
          "aa_start": 301,
          "aa_end": null,
          "aa_length": 463,
          "cds_start": 903,
          "cds_end": null,
          "cds_length": 1392,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945862.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.903C>G",
          "hgvs_p": "p.Pro301Pro",
          "transcript": "ENST00000897166.1",
          "protein_id": "ENSP00000567225.1",
          "transcript_support_level": null,
          "aa_start": 301,
          "aa_end": null,
          "aa_length": 453,
          "cds_start": 903,
          "cds_end": null,
          "cds_length": 1362,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897166.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.1053C>G",
          "hgvs_p": "p.Pro351Pro",
          "transcript": "ENST00000897161.1",
          "protein_id": "ENSP00000567220.1",
          "transcript_support_level": null,
          "aa_start": 351,
          "aa_end": null,
          "aa_length": 450,
          "cds_start": 1053,
          "cds_end": null,
          "cds_length": 1353,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897161.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.903C>G",
          "hgvs_p": "p.Pro301Pro",
          "transcript": "ENST00000945861.1",
          "protein_id": "ENSP00000615920.1",
          "transcript_support_level": null,
          "aa_start": 301,
          "aa_end": null,
          "aa_length": 447,
          "cds_start": 903,
          "cds_end": null,
          "cds_length": 1344,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945861.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.984C>G",
          "hgvs_p": "p.Pro328Pro",
          "transcript": "ENST00000945859.1",
          "protein_id": "ENSP00000615918.1",
          "transcript_support_level": null,
          "aa_start": 328,
          "aa_end": null,
          "aa_length": 427,
          "cds_start": 984,
          "cds_end": null,
          "cds_length": 1284,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945859.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.960C>G",
          "hgvs_p": "p.Pro320Pro",
          "transcript": "ENST00000897160.1",
          "protein_id": "ENSP00000567219.1",
          "transcript_support_level": null,
          "aa_start": 320,
          "aa_end": null,
          "aa_length": 419,
          "cds_start": 960,
          "cds_end": null,
          "cds_length": 1260,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897160.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 12,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.942C>G",
          "hgvs_p": "p.Pro314Pro",
          "transcript": "ENST00000920059.1",
          "protein_id": "ENSP00000590118.1",
          "transcript_support_level": null,
          "aa_start": 314,
          "aa_end": null,
          "aa_length": 413,
          "cds_start": 942,
          "cds_end": null,
          "cds_length": 1242,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920059.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.888C>G",
          "hgvs_p": "p.Pro296Pro",
          "transcript": "ENST00000920057.1",
          "protein_id": "ENSP00000590116.1",
          "transcript_support_level": null,
          "aa_start": 296,
          "aa_end": null,
          "aa_length": 395,
          "cds_start": 888,
          "cds_end": null,
          "cds_length": 1188,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920057.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.867C>G",
          "hgvs_p": "p.Pro289Pro",
          "transcript": "ENST00000897162.1",
          "protein_id": "ENSP00000567221.1",
          "transcript_support_level": null,
          "aa_start": 289,
          "aa_end": null,
          "aa_length": 388,
          "cds_start": 867,
          "cds_end": null,
          "cds_length": 1167,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897162.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.903C>G",
          "hgvs_p": "p.Pro301Pro",
          "transcript": "ENST00000920058.1",
          "protein_id": "ENSP00000590117.1",
          "transcript_support_level": null,
          "aa_start": 301,
          "aa_end": null,
          "aa_length": 385,
          "cds_start": 903,
          "cds_end": null,
          "cds_length": 1158,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000920058.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 11,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.840C>G",
          "hgvs_p": "p.Pro280Pro",
          "transcript": "ENST00000897163.1",
          "protein_id": "ENSP00000567222.1",
          "transcript_support_level": null,
          "aa_start": 280,
          "aa_end": null,
          "aa_length": 379,
          "cds_start": 840,
          "cds_end": null,
          "cds_length": 1140,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897163.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.903C>G",
          "hgvs_p": "p.Pro301Pro",
          "transcript": "ENST00000897164.1",
          "protein_id": "ENSP00000567223.1",
          "transcript_support_level": null,
          "aa_start": 301,
          "aa_end": null,
          "aa_length": 364,
          "cds_start": 903,
          "cds_end": null,
          "cds_length": 1095,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000897164.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
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          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.756C>G",
          "hgvs_p": "p.Pro252Pro",
          "transcript": "ENST00000897165.1",
          "protein_id": "ENSP00000567224.1",
          "transcript_support_level": null,
          "aa_start": 252,
          "aa_end": null,
          "aa_length": 351,
          "cds_start": 756,
          "cds_end": null,
          "cds_length": 1056,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000897165.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "synonymous_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.333C>G",
          "hgvs_p": "p.Pro111Pro",
          "transcript": "NM_001440689.1",
          "protein_id": "NP_001427618.1",
          "transcript_support_level": null,
          "aa_start": 111,
          "aa_end": null,
          "aa_length": 210,
          "cds_start": 333,
          "cds_end": null,
          "cds_length": 633,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001440689.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.705+1802C>G",
          "hgvs_p": null,
          "transcript": "NM_001440688.1",
          "protein_id": "NP_001427617.1",
          "transcript_support_level": null,
          "aa_start": null,
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          "aa_length": 307,
          "cds_start": null,
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          "cds_length": 924,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "NM_001440688.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
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          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.642+1865C>G",
          "hgvs_p": null,
          "transcript": "ENST00000945860.1",
          "protein_id": "ENSP00000615919.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 286,
          "cds_start": null,
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          "cds_length": 861,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000945860.1"
        },
        {
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          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "c.28+1802C>G",
          "hgvs_p": null,
          "transcript": "ENST00000599021.1",
          "protein_id": "ENSP00000471763.1",
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 103,
          "cds_start": null,
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          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000599021.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 10,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "MAP2K2",
          "gene_hgnc_id": 6842,
          "hgvs_c": "n.1342C>G",
          "hgvs_p": null,
          "transcript": "ENST00000394867.9",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000394867.9"
        },
        {
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        {
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          "gene_symbol": "MAP2K2",
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          "hgvs_c": "n.169+1802C>G",
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        {
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          "consequences": [
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          "gene_symbol": "MAP2K2",
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          "hgvs_c": "n.*192C>G",
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          "transcript": "ENST00000602167.5",
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          "biotype": "pseudogene",
          "feature": "ENST00000602167.5"
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      ],
      "gene_symbol": "MAP2K2",
      "gene_hgnc_id": 6842,
      "dbsnp": "rs560316877",
      "frequency_reference_population": 0.0000012486156,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 6.89843e-7,
      "gnomad_genomes_af": 0.00000657168,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": -0.8500000238418579,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "BayesDel_noAF",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": null,
      "revel_prediction": null,
      "alphamissense_score": null,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.85,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -4.031,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -5,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong,BP6_Moderate,BP7",
      "acmg_by_gene": [
        {
          "score": -5,
          "benign_score": 7,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong",
            "BP6_Moderate",
            "BP7"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_030662.4",
          "gene_symbol": "MAP2K2",
          "hgnc_id": 6842,
          "effects": [
            "synonymous_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.903C>G",
          "hgvs_p": "p.Pro301Pro"
        }
      ],
      "clinvar_disease": "RASopathy",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LB:1",
      "phenotype_combined": "RASopathy",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}