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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-41978716-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=41978716&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 41978716,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001256214.2",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP1A3",
          "gene_hgnc_id": 801,
          "hgvs_c": "c.1520G>A",
          "hgvs_p": "p.Arg507His",
          "transcript": "NM_152296.5",
          "protein_id": "NP_689509.1",
          "transcript_support_level": null,
          "aa_start": 507,
          "aa_end": null,
          "aa_length": 1013,
          "cds_start": 1520,
          "cds_end": null,
          "cds_length": 3042,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000648268.1",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_152296.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP1A3",
          "gene_hgnc_id": 801,
          "hgvs_c": "c.1520G>A",
          "hgvs_p": "p.Arg507His",
          "transcript": "ENST00000648268.1",
          "protein_id": "ENSP00000498113.1",
          "transcript_support_level": null,
          "aa_start": 507,
          "aa_end": null,
          "aa_length": 1013,
          "cds_start": 1520,
          "cds_end": null,
          "cds_length": 3042,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_152296.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000648268.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": true,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 25,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000285505",
          "gene_hgnc_id": null,
          "hgvs_c": "n.1520G>A",
          "hgvs_p": null,
          "transcript": "ENST00000644613.1",
          "protein_id": "ENSP00000494711.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "nonsense_mediated_decay",
          "feature": "ENST00000644613.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP1A3",
          "gene_hgnc_id": 801,
          "hgvs_c": "c.1559G>A",
          "hgvs_p": "p.Arg520His",
          "transcript": "NM_001256214.2",
          "protein_id": "NP_001243143.1",
          "transcript_support_level": null,
          "aa_start": 520,
          "aa_end": null,
          "aa_length": 1026,
          "cds_start": 1559,
          "cds_end": null,
          "cds_length": 3081,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001256214.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP1A3",
          "gene_hgnc_id": 801,
          "hgvs_c": "c.1559G>A",
          "hgvs_p": "p.Arg520His",
          "transcript": "ENST00000545399.6",
          "protein_id": "ENSP00000444688.1",
          "transcript_support_level": 2,
          "aa_start": 520,
          "aa_end": null,
          "aa_length": 1026,
          "cds_start": 1559,
          "cds_end": null,
          "cds_length": 3081,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000545399.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP1A3",
          "gene_hgnc_id": 801,
          "hgvs_c": "c.1553G>A",
          "hgvs_p": "p.Arg518His",
          "transcript": "NM_001256213.2",
          "protein_id": "NP_001243142.1",
          "transcript_support_level": null,
          "aa_start": 518,
          "aa_end": null,
          "aa_length": 1024,
          "cds_start": 1553,
          "cds_end": null,
          "cds_length": 3075,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001256213.2"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP1A3",
          "gene_hgnc_id": 801,
          "hgvs_c": "c.1553G>A",
          "hgvs_p": "p.Arg518His",
          "transcript": "ENST00000543770.5",
          "protein_id": "ENSP00000437577.1",
          "transcript_support_level": 2,
          "aa_start": 518,
          "aa_end": null,
          "aa_length": 1024,
          "cds_start": 1553,
          "cds_end": null,
          "cds_length": 3075,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000543770.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP1A3",
          "gene_hgnc_id": 801,
          "hgvs_c": "c.1520G>A",
          "hgvs_p": "p.Arg507His",
          "transcript": "ENST00000890880.1",
          "protein_id": "ENSP00000560939.1",
          "transcript_support_level": null,
          "aa_start": 507,
          "aa_end": null,
          "aa_length": 996,
          "cds_start": 1520,
          "cds_end": null,
          "cds_length": 2991,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890880.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP1A3",
          "gene_hgnc_id": 801,
          "hgvs_c": "c.1520G>A",
          "hgvs_p": "p.Arg507His",
          "transcript": "ENST00000890884.1",
          "protein_id": "ENSP00000560943.1",
          "transcript_support_level": null,
          "aa_start": 507,
          "aa_end": null,
          "aa_length": 994,
          "cds_start": 1520,
          "cds_end": null,
          "cds_length": 2985,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890884.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP1A3",
          "gene_hgnc_id": 801,
          "hgvs_c": "c.1520G>A",
          "hgvs_p": "p.Arg507His",
          "transcript": "ENST00000919566.1",
          "protein_id": "ENSP00000589625.1",
          "transcript_support_level": null,
          "aa_start": 507,
          "aa_end": null,
          "aa_length": 984,
          "cds_start": 1520,
          "cds_end": null,
          "cds_length": 2955,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000919566.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP1A3",
          "gene_hgnc_id": 801,
          "hgvs_c": "c.1430G>A",
          "hgvs_p": "p.Arg477His",
          "transcript": "ENST00000602133.5",
          "protein_id": "ENSP00000471581.1",
          "transcript_support_level": 2,
          "aa_start": 477,
          "aa_end": null,
          "aa_length": 983,
          "cds_start": 1430,
          "cds_end": null,
          "cds_length": 2952,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000602133.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 11,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP1A3",
          "gene_hgnc_id": 801,
          "hgvs_c": "c.1316G>A",
          "hgvs_p": "p.Arg439His",
          "transcript": "ENST00000890882.1",
          "protein_id": "ENSP00000560941.1",
          "transcript_support_level": null,
          "aa_start": 439,
          "aa_end": null,
          "aa_length": 945,
          "cds_start": 1316,
          "cds_end": null,
          "cds_length": 2838,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000890882.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP1A3",
          "gene_hgnc_id": 801,
          "hgvs_c": "c.1007G>A",
          "hgvs_p": "p.Arg336His",
          "transcript": "ENST00000948333.1",
          "protein_id": "ENSP00000618392.1",
          "transcript_support_level": null,
          "aa_start": 336,
          "aa_end": null,
          "aa_length": 842,
          "cds_start": 1007,
          "cds_end": null,
          "cds_length": 2529,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000948333.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "H",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 23,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP1A3",
          "gene_hgnc_id": 801,
          "hgvs_c": "c.1430G>A",
          "hgvs_p": "p.Arg477His",
          "transcript": "XM_047438862.1",
          "protein_id": "XP_047294818.1",
          "transcript_support_level": null,
          "aa_start": 477,
          "aa_end": null,
          "aa_length": 983,
          "cds_start": 1430,
          "cds_end": null,
          "cds_length": 2952,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_047438862.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ATP1A3",
          "gene_hgnc_id": 801,
          "hgvs_c": "n.1520G>A",
          "hgvs_p": null,
          "transcript": "ENST00000441343.5",
          "protein_id": "ENSP00000411503.1",
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "non_stop_decay",
          "feature": "ENST00000441343.5"
        }
      ],
      "gene_symbol": "ATP1A3",
      "gene_hgnc_id": 801,
      "dbsnp": "rs782370873",
      "frequency_reference_population": 0.00017348246,
      "hom_count_reference_population": 1,
      "allele_count_reference_population": 280,
      "gnomad_exomes_af": 0.000187435,
      "gnomad_genomes_af": 0.0000394327,
      "gnomad_exomes_ac": 274,
      "gnomad_genomes_ac": 6,
      "gnomad_exomes_homalt": 1,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.7642908096313477,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.03999999910593033,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.685,
      "revel_prediction": "Pathogenic",
      "alphamissense_score": 0.4521,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.05,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 5.918,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.04,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 1,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PP2,PP3,BS1_Supporting",
      "acmg_by_gene": [
        {
          "score": 1,
          "benign_score": 1,
          "pathogenic_score": 2,
          "criteria": [
            "PP2",
            "PP3",
            "BS1_Supporting"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001256214.2",
          "gene_symbol": "ATP1A3",
          "hgnc_id": 801,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD,AR",
          "hgvs_c": "c.1559G>A",
          "hgvs_p": "p.Arg520His"
        },
        {
          "score": 1,
          "benign_score": 0,
          "pathogenic_score": 1,
          "criteria": [
            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000644613.1",
          "gene_symbol": "ENSG00000285505",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.1520G>A",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Dystonia 12,not provided",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:2",
      "phenotype_combined": "Dystonia 12|not provided",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}