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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-41999226-C-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=41999226&ref=C&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 41999226,
      "ref": "C",
      "alt": "G",
      "effect": "missense_variant",
      "transcript": "NM_002088.5",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2588G>C",
          "hgvs_p": "p.Arg863Pro",
          "transcript": "NM_002088.5",
          "protein_id": "NP_002079.3",
          "transcript_support_level": null,
          "aa_start": 863,
          "aa_end": null,
          "aa_length": 980,
          "cds_start": 2588,
          "cds_end": null,
          "cds_length": 2943,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000593562.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002088.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2588G>C",
          "hgvs_p": "p.Arg863Pro",
          "transcript": "ENST00000593562.6",
          "protein_id": "ENSP00000470251.1",
          "transcript_support_level": 5,
          "aa_start": 863,
          "aa_end": null,
          "aa_length": 980,
          "cds_start": 2588,
          "cds_end": null,
          "cds_length": 2943,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_002088.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000593562.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2588G>C",
          "hgvs_p": "p.Arg863Pro",
          "transcript": "ENST00000262895.7",
          "protein_id": "ENSP00000262895.2",
          "transcript_support_level": 1,
          "aa_start": 863,
          "aa_end": null,
          "aa_length": 980,
          "cds_start": 2588,
          "cds_end": null,
          "cds_length": 2943,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000262895.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "n.1465G>C",
          "hgvs_p": null,
          "transcript": "ENST00000454993.6",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000454993.6"
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2627G>C",
          "hgvs_p": "p.Arg876Pro",
          "transcript": "ENST00000933564.1",
          "protein_id": "ENSP00000603623.1",
          "transcript_support_level": null,
          "aa_start": 876,
          "aa_end": null,
          "aa_length": 993,
          "cds_start": 2627,
          "cds_end": null,
          "cds_length": 2982,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000933564.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2603G>C",
          "hgvs_p": "p.Arg868Pro",
          "transcript": "ENST00000933563.1",
          "protein_id": "ENSP00000603622.1",
          "transcript_support_level": null,
          "aa_start": 868,
          "aa_end": null,
          "aa_length": 985,
          "cds_start": 2603,
          "cds_end": null,
          "cds_length": 2958,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000933563.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2573G>C",
          "hgvs_p": "p.Arg858Pro",
          "transcript": "ENST00000933560.1",
          "protein_id": "ENSP00000603619.1",
          "transcript_support_level": null,
          "aa_start": 858,
          "aa_end": null,
          "aa_length": 975,
          "cds_start": 2573,
          "cds_end": null,
          "cds_length": 2928,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000933560.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2474G>C",
          "hgvs_p": "p.Arg825Pro",
          "transcript": "ENST00000933561.1",
          "protein_id": "ENSP00000603620.1",
          "transcript_support_level": null,
          "aa_start": 825,
          "aa_end": null,
          "aa_length": 942,
          "cds_start": 2474,
          "cds_end": null,
          "cds_length": 2829,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000933561.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.1811G>C",
          "hgvs_p": "p.Arg604Pro",
          "transcript": "ENST00000933562.1",
          "protein_id": "ENSP00000603621.1",
          "transcript_support_level": null,
          "aa_start": 604,
          "aa_end": null,
          "aa_length": 721,
          "cds_start": 1811,
          "cds_end": null,
          "cds_length": 2166,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000933562.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "P",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2591G>C",
          "hgvs_p": "p.Arg864Pro",
          "transcript": "XM_011526862.3",
          "protein_id": "XP_011525164.1",
          "transcript_support_level": null,
          "aa_start": 864,
          "aa_end": null,
          "aa_length": 981,
          "cds_start": 2591,
          "cds_end": null,
          "cds_length": 2946,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011526862.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "downstream_gene_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "n.*17G>C",
          "hgvs_p": null,
          "transcript": "ENST00000602210.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000602210.1"
        }
      ],
      "gene_symbol": "GRIK5",
      "gene_hgnc_id": 4583,
      "dbsnp": "rs541637785",
      "frequency_reference_population": 0.0000032864336,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 5,
      "gnomad_exomes_af": 0.00000146049,
      "gnomad_genomes_af": 0.0000197361,
      "gnomad_exomes_ac": 2,
      "gnomad_genomes_ac": 3,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.16642147302627563,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.094,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1873,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.46,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 5.344,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 2,
          "pathogenic_score": 0,
          "criteria": [
            "BP4_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_002088.5",
          "gene_symbol": "GRIK5",
          "hgnc_id": 4583,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.2588G>C",
          "hgvs_p": "p.Arg863Pro"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}