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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-41999249-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=41999249&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 41999249,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_002088.5",
      "consequences": [
        {
          "aa_ref": "C",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2565C>G",
          "hgvs_p": "p.Cys855Trp",
          "transcript": "NM_002088.5",
          "protein_id": "NP_002079.3",
          "transcript_support_level": null,
          "aa_start": 855,
          "aa_end": null,
          "aa_length": 980,
          "cds_start": 2565,
          "cds_end": null,
          "cds_length": 2943,
          "cdna_start": 3581,
          "cdna_end": null,
          "cdna_length": 4506,
          "mane_select": "ENST00000593562.6",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_002088.5"
        },
        {
          "aa_ref": "C",
          "aa_alt": "W",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2565C>G",
          "hgvs_p": "p.Cys855Trp",
          "transcript": "ENST00000593562.6",
          "protein_id": "ENSP00000470251.1",
          "transcript_support_level": 5,
          "aa_start": 855,
          "aa_end": null,
          "aa_length": 980,
          "cds_start": 2565,
          "cds_end": null,
          "cds_length": 2943,
          "cdna_start": 3581,
          "cdna_end": null,
          "cdna_length": 4506,
          "mane_select": "NM_002088.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000593562.6"
        },
        {
          "aa_ref": "C",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2565C>G",
          "hgvs_p": "p.Cys855Trp",
          "transcript": "ENST00000262895.7",
          "protein_id": "ENSP00000262895.2",
          "transcript_support_level": 1,
          "aa_start": 855,
          "aa_end": null,
          "aa_length": 980,
          "cds_start": 2565,
          "cds_end": null,
          "cds_length": 2943,
          "cdna_start": 2565,
          "cdna_end": null,
          "cdna_length": 3493,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000262895.7"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "n.1442C>G",
          "hgvs_p": null,
          "transcript": "ENST00000454993.6",
          "protein_id": null,
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 2370,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000454993.6"
        },
        {
          "aa_ref": "C",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2604C>G",
          "hgvs_p": "p.Cys868Trp",
          "transcript": "ENST00000933564.1",
          "protein_id": "ENSP00000603623.1",
          "transcript_support_level": null,
          "aa_start": 868,
          "aa_end": null,
          "aa_length": 993,
          "cds_start": 2604,
          "cds_end": null,
          "cds_length": 2982,
          "cdna_start": 2701,
          "cdna_end": null,
          "cdna_length": 3626,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000933564.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2580C>G",
          "hgvs_p": "p.Cys860Trp",
          "transcript": "ENST00000933563.1",
          "protein_id": "ENSP00000603622.1",
          "transcript_support_level": null,
          "aa_start": 860,
          "aa_end": null,
          "aa_length": 985,
          "cds_start": 2580,
          "cds_end": null,
          "cds_length": 2958,
          "cdna_start": 2926,
          "cdna_end": null,
          "cdna_length": 3849,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000933563.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2550C>G",
          "hgvs_p": "p.Cys850Trp",
          "transcript": "ENST00000933560.1",
          "protein_id": "ENSP00000603619.1",
          "transcript_support_level": null,
          "aa_start": 850,
          "aa_end": null,
          "aa_length": 975,
          "cds_start": 2550,
          "cds_end": null,
          "cds_length": 2928,
          "cdna_start": 3600,
          "cdna_end": null,
          "cdna_length": 4528,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000933560.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2451C>G",
          "hgvs_p": "p.Cys817Trp",
          "transcript": "ENST00000933561.1",
          "protein_id": "ENSP00000603620.1",
          "transcript_support_level": null,
          "aa_start": 817,
          "aa_end": null,
          "aa_length": 942,
          "cds_start": 2451,
          "cds_end": null,
          "cds_length": 2829,
          "cdna_start": 3463,
          "cdna_end": null,
          "cdna_length": 4396,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000933561.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.1788C>G",
          "hgvs_p": "p.Cys596Trp",
          "transcript": "ENST00000933562.1",
          "protein_id": "ENSP00000603621.1",
          "transcript_support_level": null,
          "aa_start": 596,
          "aa_end": null,
          "aa_length": 721,
          "cds_start": 1788,
          "cds_end": null,
          "cds_length": 2166,
          "cdna_start": 2175,
          "cdna_end": null,
          "cdna_length": 3095,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000933562.1"
        },
        {
          "aa_ref": "C",
          "aa_alt": "W",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "c.2568C>G",
          "hgvs_p": "p.Cys856Trp",
          "transcript": "XM_011526862.3",
          "protein_id": "XP_011525164.1",
          "transcript_support_level": null,
          "aa_start": 856,
          "aa_end": null,
          "aa_length": 981,
          "cds_start": 2568,
          "cds_end": null,
          "cds_length": 2946,
          "cdna_start": 3572,
          "cdna_end": null,
          "cdna_length": 4497,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_011526862.3"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": false,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "GRIK5",
          "gene_hgnc_id": 4583,
          "hgvs_c": "n.383C>G",
          "hgvs_p": null,
          "transcript": "ENST00000602210.1",
          "protein_id": null,
          "transcript_support_level": 2,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 389,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "retained_intron",
          "feature": "ENST00000602210.1"
        }
      ],
      "gene_symbol": "GRIK5",
      "gene_hgnc_id": 4583,
      "dbsnp": "rs1193861383",
      "frequency_reference_population": 0.000011149413,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 17,
      "gnomad_exomes_af": 0.0000109277,
      "gnomad_genomes_af": 0.0000131508,
      "gnomad_exomes_ac": 15,
      "gnomad_genomes_ac": 2,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.48945480585098267,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.169,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.8652,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.32,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.678,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BS2",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 4,
          "pathogenic_score": 0,
          "criteria": [
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_002088.5",
          "gene_symbol": "GRIK5",
          "hgnc_id": 4583,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.2565C>G",
          "hgvs_p": "p.Cys855Trp"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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