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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-4523930-G-C (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=4523930&ref=G&alt=C&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 4523930,
      "ref": "G",
      "alt": "C",
      "effect": "missense_variant",
      "transcript": "NM_001013706.3",
      "consequences": [
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.990C>G",
          "hgvs_p": "p.Phe330Leu",
          "transcript": "NM_001013706.3",
          "protein_id": "NP_001013728.2",
          "transcript_support_level": null,
          "aa_start": 330,
          "aa_end": null,
          "aa_length": 463,
          "cds_start": 990,
          "cds_end": null,
          "cds_length": 1392,
          "cdna_start": 1071,
          "cdna_end": null,
          "cdna_length": 2470,
          "mane_select": "ENST00000381848.7",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001013706.3"
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.990C>G",
          "hgvs_p": "p.Phe330Leu",
          "transcript": "ENST00000381848.7",
          "protein_id": "ENSP00000371272.2",
          "transcript_support_level": 1,
          "aa_start": 330,
          "aa_end": null,
          "aa_length": 463,
          "cds_start": 990,
          "cds_end": null,
          "cds_length": 1392,
          "cdna_start": 1071,
          "cdna_end": null,
          "cdna_length": 2470,
          "mane_select": "NM_001013706.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000381848.7"
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.1227C>G",
          "hgvs_p": "p.Phe409Leu",
          "transcript": "ENST00000905186.1",
          "protein_id": "ENSP00000575245.1",
          "transcript_support_level": null,
          "aa_start": 409,
          "aa_end": null,
          "aa_length": 542,
          "cds_start": 1227,
          "cds_end": null,
          "cds_length": 1629,
          "cdna_start": 1312,
          "cdna_end": null,
          "cdna_length": 2163,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000905186.1"
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.1191C>G",
          "hgvs_p": "p.Phe397Leu",
          "transcript": "ENST00000905182.1",
          "protein_id": "ENSP00000575241.1",
          "transcript_support_level": null,
          "aa_start": 397,
          "aa_end": null,
          "aa_length": 530,
          "cds_start": 1191,
          "cds_end": null,
          "cds_length": 1593,
          "cdna_start": 1295,
          "cdna_end": null,
          "cdna_length": 2694,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000905182.1"
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.1110C>G",
          "hgvs_p": "p.Phe370Leu",
          "transcript": "ENST00000942350.1",
          "protein_id": "ENSP00000612409.1",
          "transcript_support_level": null,
          "aa_start": 370,
          "aa_end": null,
          "aa_length": 503,
          "cds_start": 1110,
          "cds_end": null,
          "cds_length": 1512,
          "cdna_start": 1191,
          "cdna_end": null,
          "cdna_length": 2587,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942350.1"
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.1110C>G",
          "hgvs_p": "p.Phe370Leu",
          "transcript": "ENST00000942352.1",
          "protein_id": "ENSP00000612411.1",
          "transcript_support_level": null,
          "aa_start": 370,
          "aa_end": null,
          "aa_length": 503,
          "cds_start": 1110,
          "cds_end": null,
          "cds_length": 1512,
          "cdna_start": 1190,
          "cdna_end": null,
          "cdna_length": 2586,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942352.1"
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.1095C>G",
          "hgvs_p": "p.Phe365Leu",
          "transcript": "ENST00000942351.1",
          "protein_id": "ENSP00000612410.1",
          "transcript_support_level": null,
          "aa_start": 365,
          "aa_end": null,
          "aa_length": 498,
          "cds_start": 1095,
          "cds_end": null,
          "cds_length": 1497,
          "cdna_start": 1176,
          "cdna_end": null,
          "cdna_length": 2572,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000942351.1"
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.1074C>G",
          "hgvs_p": "p.Phe358Leu",
          "transcript": "ENST00000905187.1",
          "protein_id": "ENSP00000575246.1",
          "transcript_support_level": null,
          "aa_start": 358,
          "aa_end": null,
          "aa_length": 491,
          "cds_start": 1074,
          "cds_end": null,
          "cds_length": 1476,
          "cdna_start": 1159,
          "cdna_end": null,
          "cdna_length": 2010,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000905187.1"
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 9,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.1056C>G",
          "hgvs_p": "p.Phe352Leu",
          "transcript": "ENST00000905193.1",
          "protein_id": "ENSP00000575252.1",
          "transcript_support_level": null,
          "aa_start": 352,
          "aa_end": null,
          "aa_length": 485,
          "cds_start": 1056,
          "cds_end": null,
          "cds_length": 1458,
          "cdna_start": 1137,
          "cdna_end": null,
          "cdna_length": 1965,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000905193.1"
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.1047C>G",
          "hgvs_p": "p.Phe349Leu",
          "transcript": "ENST00000905188.1",
          "protein_id": "ENSP00000575247.1",
          "transcript_support_level": null,
          "aa_start": 349,
          "aa_end": null,
          "aa_length": 482,
          "cds_start": 1047,
          "cds_end": null,
          "cds_length": 1449,
          "cdna_start": 1132,
          "cdna_end": null,
          "cdna_length": 1983,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000905188.1"
        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.1026C>G",
          "hgvs_p": "p.Phe342Leu",
          "transcript": "ENST00000905183.1",
          "protein_id": "ENSP00000575242.1",
          "transcript_support_level": null,
          "aa_start": 342,
          "aa_end": null,
          "aa_length": 475,
          "cds_start": 1026,
          "cds_end": null,
          "cds_length": 1428,
          "cdna_start": 1111,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "F",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 9,
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          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.1026C>G",
          "hgvs_p": "p.Phe342Leu",
          "transcript": "ENST00000942353.1",
          "protein_id": "ENSP00000612412.1",
          "transcript_support_level": null,
          "aa_start": 342,
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          "aa_length": 475,
          "cds_start": 1026,
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          "cdna_start": 1107,
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          "cdna_length": 1969,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "F",
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          "protein_coding": true,
          "strand": false,
          "consequences": [
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          ],
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "PLIN5",
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          "hgvs_c": "c.1011C>G",
          "hgvs_p": "p.Phe337Leu",
          "transcript": "ENST00000905184.1",
          "protein_id": "ENSP00000575243.1",
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          "aa_start": 337,
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          "cds_start": 1011,
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          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000905184.1"
        },
        {
          "aa_ref": "F",
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          "strand": false,
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            "missense_variant"
          ],
          "exon_rank": 8,
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.1011C>G",
          "hgvs_p": "p.Phe337Leu",
          "transcript": "ENST00000905192.1",
          "protein_id": "ENSP00000575251.1",
          "transcript_support_level": null,
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        {
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          "gene_symbol": "PLIN5",
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          "hgvs_c": "c.990C>G",
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          "transcript": "ENST00000905191.1",
          "protein_id": "ENSP00000575250.1",
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          "cdna_start": 1058,
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          "cdna_length": 1886,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000905191.1"
        },
        {
          "aa_ref": "F",
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          "strand": false,
          "consequences": [
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          ],
          "exon_rank": 9,
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          "exon_count": 9,
          "intron_rank": null,
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          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.990C>G",
          "hgvs_p": "p.Phe330Leu",
          "transcript": "ENST00000905194.1",
          "protein_id": "ENSP00000575253.1",
          "transcript_support_level": null,
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        {
          "aa_ref": "F",
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          ],
          "exon_rank": 8,
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          "exon_count": 8,
          "intron_rank": null,
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          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.990C>G",
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        {
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          "gene_symbol": "PLIN5",
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          "hgvs_c": "c.990C>G",
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          "protein_id": "ENSP00000612414.1",
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        {
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          ],
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          "gene_symbol": "PLIN5",
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          "hgvs_c": "c.978C>G",
          "hgvs_p": "p.Phe326Leu",
          "transcript": "ENST00000905190.1",
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          "aa_start": 326,
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          "cds_start": 978,
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          "cds_length": 1380,
          "cdna_start": 1057,
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          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "F",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLIN5",
          "gene_hgnc_id": 33196,
          "hgvs_c": "c.894C>G",
          "hgvs_p": "p.Phe298Leu",
          "transcript": "ENST00000905185.1",
          "protein_id": "ENSP00000575244.1",
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          "aa_start": 298,
          "aa_end": null,
          "aa_length": 431,
          "cds_start": 894,
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          "cds_length": 1296,
          "cdna_start": 979,
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        {
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        {
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        {
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          "exon_count": 3,
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          "gene_symbol": "PLIN5",
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          "hgvs_c": "n.487C>G",
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          "transcript": "ENST00000589728.1",
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          "cdna_length": 508,
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          "biotype": "retained_intron",
          "feature": "ENST00000589728.1"
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      ],
      "gene_symbol": "PLIN5",
      "gene_hgnc_id": 33196,
      "dbsnp": "rs764528049",
      "frequency_reference_population": 0.000024196797,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 37,
      "gnomad_exomes_af": 0.00000871572,
      "gnomad_genomes_af": 0.000164143,
      "gnomad_exomes_ac": 12,
      "gnomad_genomes_ac": 25,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.3925289809703827,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.13,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.9092,
      "alphamissense_prediction": "Pathogenic",
      "bayesdelnoaf_score": -0.55,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.113,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -1,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "BP4",
      "acmg_by_gene": [
        {
          "score": -1,
          "benign_score": 1,
          "pathogenic_score": 0,
          "criteria": [
            "BP4"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001013706.3",
          "gene_symbol": "PLIN5",
          "hgnc_id": 33196,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.990C>G",
          "hgvs_p": "p.Phe330Leu"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}
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