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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-45307628-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=45307628&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 45307628,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001824.5",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.800C>T",
          "hgvs_p": "p.Ala267Val",
          "transcript": "NM_001824.5",
          "protein_id": "NP_001815.2",
          "transcript_support_level": null,
          "aa_start": 267,
          "aa_end": null,
          "aa_length": 381,
          "cds_start": 800,
          "cds_end": null,
          "cds_length": 1146,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000221476.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001824.5"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.800C>T",
          "hgvs_p": "p.Ala267Val",
          "transcript": "ENST00000221476.4",
          "protein_id": "ENSP00000221476.2",
          "transcript_support_level": 1,
          "aa_start": 267,
          "aa_end": null,
          "aa_length": 381,
          "cds_start": 800,
          "cds_end": null,
          "cds_length": 1146,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001824.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000221476.4"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.953C>T",
          "hgvs_p": "p.Ala318Val",
          "transcript": "ENST00000969560.1",
          "protein_id": "ENSP00000639619.1",
          "transcript_support_level": null,
          "aa_start": 318,
          "aa_end": null,
          "aa_length": 432,
          "cds_start": 953,
          "cds_end": null,
          "cds_length": 1299,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000969560.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.917C>T",
          "hgvs_p": "p.Ala306Val",
          "transcript": "ENST00000969562.1",
          "protein_id": "ENSP00000639621.1",
          "transcript_support_level": null,
          "aa_start": 306,
          "aa_end": null,
          "aa_length": 420,
          "cds_start": 917,
          "cds_end": null,
          "cds_length": 1263,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000969562.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.833C>T",
          "hgvs_p": "p.Ala278Val",
          "transcript": "ENST00000969561.1",
          "protein_id": "ENSP00000639620.1",
          "transcript_support_level": null,
          "aa_start": 278,
          "aa_end": null,
          "aa_length": 392,
          "cds_start": 833,
          "cds_end": null,
          "cds_length": 1179,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000969561.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.800C>T",
          "hgvs_p": "p.Ala267Val",
          "transcript": "ENST00000859489.1",
          "protein_id": "ENSP00000529548.1",
          "transcript_support_level": null,
          "aa_start": 267,
          "aa_end": null,
          "aa_length": 381,
          "cds_start": 800,
          "cds_end": null,
          "cds_length": 1146,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859489.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.800C>T",
          "hgvs_p": "p.Ala267Val",
          "transcript": "ENST00000969553.1",
          "protein_id": "ENSP00000639612.1",
          "transcript_support_level": null,
          "aa_start": 267,
          "aa_end": null,
          "aa_length": 381,
          "cds_start": 800,
          "cds_end": null,
          "cds_length": 1146,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000969553.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.800C>T",
          "hgvs_p": "p.Ala267Val",
          "transcript": "ENST00000969554.1",
          "protein_id": "ENSP00000639613.1",
          "transcript_support_level": null,
          "aa_start": 267,
          "aa_end": null,
          "aa_length": 381,
          "cds_start": 800,
          "cds_end": null,
          "cds_length": 1146,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000969554.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 8,
          "exon_rank_end": null,
          "exon_count": 9,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.800C>T",
          "hgvs_p": "p.Ala267Val",
          "transcript": "ENST00000969559.1",
          "protein_id": "ENSP00000639618.1",
          "transcript_support_level": null,
          "aa_start": 267,
          "aa_end": null,
          "aa_length": 381,
          "cds_start": 800,
          "cds_end": null,
          "cds_length": 1146,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000969559.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.800C>T",
          "hgvs_p": "p.Ala267Val",
          "transcript": "ENST00000969563.1",
          "protein_id": "ENSP00000639622.1",
          "transcript_support_level": null,
          "aa_start": 267,
          "aa_end": null,
          "aa_length": 381,
          "cds_start": 800,
          "cds_end": null,
          "cds_length": 1146,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000969563.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.797C>T",
          "hgvs_p": "p.Ala266Val",
          "transcript": "ENST00000969555.1",
          "protein_id": "ENSP00000639614.1",
          "transcript_support_level": null,
          "aa_start": 266,
          "aa_end": null,
          "aa_length": 380,
          "cds_start": 797,
          "cds_end": null,
          "cds_length": 1143,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000969555.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.800C>T",
          "hgvs_p": "p.Ala267Val",
          "transcript": "ENST00000859491.1",
          "protein_id": "ENSP00000529550.1",
          "transcript_support_level": null,
          "aa_start": 267,
          "aa_end": null,
          "aa_length": 379,
          "cds_start": 800,
          "cds_end": null,
          "cds_length": 1140,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859491.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.794C>T",
          "hgvs_p": "p.Ala265Val",
          "transcript": "ENST00000969558.1",
          "protein_id": "ENSP00000639617.1",
          "transcript_support_level": null,
          "aa_start": 265,
          "aa_end": null,
          "aa_length": 379,
          "cds_start": 794,
          "cds_end": null,
          "cds_length": 1140,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000969558.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.791C>T",
          "hgvs_p": "p.Ala264Val",
          "transcript": "ENST00000969556.1",
          "protein_id": "ENSP00000639615.1",
          "transcript_support_level": null,
          "aa_start": 264,
          "aa_end": null,
          "aa_length": 378,
          "cds_start": 791,
          "cds_end": null,
          "cds_length": 1137,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000969556.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.800C>T",
          "hgvs_p": "p.Ala267Val",
          "transcript": "ENST00000969557.1",
          "protein_id": "ENSP00000639616.1",
          "transcript_support_level": null,
          "aa_start": 267,
          "aa_end": null,
          "aa_length": 376,
          "cds_start": 800,
          "cds_end": null,
          "cds_length": 1131,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000969557.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.800C>T",
          "hgvs_p": "p.Ala267Val",
          "transcript": "ENST00000969564.1",
          "protein_id": "ENSP00000639623.1",
          "transcript_support_level": null,
          "aa_start": 267,
          "aa_end": null,
          "aa_length": 328,
          "cds_start": 800,
          "cds_end": null,
          "cds_length": 987,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000969564.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.512C>T",
          "hgvs_p": "p.Ala171Val",
          "transcript": "ENST00000859490.1",
          "protein_id": "ENSP00000529549.1",
          "transcript_support_level": null,
          "aa_start": 171,
          "aa_end": null,
          "aa_length": 285,
          "cds_start": 512,
          "cds_end": null,
          "cds_length": 858,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859490.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "V",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.305C>T",
          "hgvs_p": "p.Ala102Val",
          "transcript": "ENST00000859493.1",
          "protein_id": "ENSP00000529552.1",
          "transcript_support_level": null,
          "aa_start": 102,
          "aa_end": null,
          "aa_length": 216,
          "cds_start": 305,
          "cds_end": null,
          "cds_length": 651,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859493.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 8,
          "intron_rank": 6,
          "intron_rank_end": null,
          "gene_symbol": "CKM",
          "gene_hgnc_id": 1994,
          "hgvs_c": "c.778-26C>T",
          "hgvs_p": null,
          "transcript": "ENST00000859492.1",
          "protein_id": "ENSP00000529551.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 365,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1098,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000859492.1"
        }
      ],
      "gene_symbol": "CKM",
      "gene_hgnc_id": 1994,
      "dbsnp": "rs138693691",
      "frequency_reference_population": 0.0000061572227,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 9,
      "gnomad_exomes_af": 0.00000615722,
      "gnomad_genomes_af": null,
      "gnomad_exomes_ac": 9,
      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.24603289365768433,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.067,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1501,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.46,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 2.687,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,BP4_Moderate",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 2,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001824.5",
          "gene_symbol": "CKM",
          "hgnc_id": 1994,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.800C>T",
          "hgvs_p": "p.Ala267Val"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}