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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-45616558-T-G (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=45616558&ref=T&alt=G&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 45616558,
      "ref": "T",
      "alt": "G",
      "effect": "missense_variant,splice_region_variant",
      "transcript": "NM_001193268.3",
      "consequences": [
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.1412A>C",
          "hgvs_p": "p.Asp471Ala",
          "transcript": "NM_012155.4",
          "protein_id": "NP_036287.1",
          "transcript_support_level": null,
          "aa_start": 471,
          "aa_end": null,
          "aa_length": 649,
          "cds_start": 1412,
          "cds_end": null,
          "cds_length": 1950,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000245925.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_012155.4"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.1412A>C",
          "hgvs_p": "p.Asp471Ala",
          "transcript": "ENST00000245925.8",
          "protein_id": "ENSP00000245925.3",
          "transcript_support_level": 1,
          "aa_start": 471,
          "aa_end": null,
          "aa_length": 649,
          "cds_start": 1412,
          "cds_end": null,
          "cds_length": 1950,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_012155.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000245925.8"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.1412A>C",
          "hgvs_p": "p.Asp471Ala",
          "transcript": "ENST00000589876.5",
          "protein_id": "ENSP00000464789.1",
          "transcript_support_level": 1,
          "aa_start": 471,
          "aa_end": null,
          "aa_length": 773,
          "cds_start": 1412,
          "cds_end": null,
          "cds_length": 2322,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000589876.5"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.2015A>C",
          "hgvs_p": "p.Asp672Ala",
          "transcript": "NM_001193268.3",
          "protein_id": "NP_001180197.1",
          "transcript_support_level": null,
          "aa_start": 672,
          "aa_end": null,
          "aa_length": 850,
          "cds_start": 2015,
          "cds_end": null,
          "cds_length": 2553,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001193268.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.2015A>C",
          "hgvs_p": "p.Asp672Ala",
          "transcript": "ENST00000587152.6",
          "protein_id": "ENSP00000468312.1",
          "transcript_support_level": 2,
          "aa_start": 672,
          "aa_end": null,
          "aa_length": 850,
          "cds_start": 2015,
          "cds_end": null,
          "cds_length": 2553,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000587152.6"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.2012A>C",
          "hgvs_p": "p.Asp671Ala",
          "transcript": "NM_001352052.1",
          "protein_id": "NP_001338981.1",
          "transcript_support_level": null,
          "aa_start": 671,
          "aa_end": null,
          "aa_length": 849,
          "cds_start": 2012,
          "cds_end": null,
          "cds_length": 2550,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001352052.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.1853A>C",
          "hgvs_p": "p.Asp618Ala",
          "transcript": "NM_001193269.2",
          "protein_id": "NP_001180198.1",
          "transcript_support_level": null,
          "aa_start": 618,
          "aa_end": null,
          "aa_length": 796,
          "cds_start": 1853,
          "cds_end": null,
          "cds_length": 2391,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001193269.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.1853A>C",
          "hgvs_p": "p.Asp618Ala",
          "transcript": "ENST00000536630.5",
          "protein_id": "ENSP00000442365.1",
          "transcript_support_level": 2,
          "aa_start": 618,
          "aa_end": null,
          "aa_length": 796,
          "cds_start": 1853,
          "cds_end": null,
          "cds_length": 2391,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000536630.5"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 22,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.1850A>C",
          "hgvs_p": "p.Asp617Ala",
          "transcript": "NM_001352053.2",
          "protein_id": "NP_001338982.1",
          "transcript_support_level": null,
          "aa_start": 617,
          "aa_end": null,
          "aa_length": 795,
          "cds_start": 1850,
          "cds_end": null,
          "cds_length": 2388,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001352053.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 16,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.1517A>C",
          "hgvs_p": "p.Asp506Ala",
          "transcript": "ENST00000905380.1",
          "protein_id": "ENSP00000575439.1",
          "transcript_support_level": null,
          "aa_start": 506,
          "aa_end": null,
          "aa_length": 684,
          "cds_start": 1517,
          "cds_end": null,
          "cds_length": 2055,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000905380.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.1436A>C",
          "hgvs_p": "p.Asp479Ala",
          "transcript": "ENST00000905379.1",
          "protein_id": "ENSP00000575438.1",
          "transcript_support_level": null,
          "aa_start": 479,
          "aa_end": null,
          "aa_length": 657,
          "cds_start": 1436,
          "cds_end": null,
          "cds_length": 1974,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000905379.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.1355A>C",
          "hgvs_p": "p.Asp452Ala",
          "transcript": "ENST00000905381.1",
          "protein_id": "ENSP00000575440.1",
          "transcript_support_level": null,
          "aa_start": 452,
          "aa_end": null,
          "aa_length": 630,
          "cds_start": 1355,
          "cds_end": null,
          "cds_length": 1893,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000905381.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.1346A>C",
          "hgvs_p": "p.Asp449Ala",
          "transcript": "NM_001352054.2",
          "protein_id": "NP_001338983.1",
          "transcript_support_level": null,
          "aa_start": 449,
          "aa_end": null,
          "aa_length": 627,
          "cds_start": 1346,
          "cds_end": null,
          "cds_length": 1884,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001352054.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.1277A>C",
          "hgvs_p": "p.Asp426Ala",
          "transcript": "ENST00000905378.1",
          "protein_id": "ENSP00000575437.1",
          "transcript_support_level": null,
          "aa_start": 426,
          "aa_end": null,
          "aa_length": 604,
          "cds_start": 1277,
          "cds_end": null,
          "cds_length": 1815,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000905378.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.1256A>C",
          "hgvs_p": "p.Asp419Ala",
          "transcript": "ENST00000905382.1",
          "protein_id": "ENSP00000575441.1",
          "transcript_support_level": null,
          "aa_start": 419,
          "aa_end": null,
          "aa_length": 597,
          "cds_start": 1256,
          "cds_end": null,
          "cds_length": 1794,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000905382.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.1157A>C",
          "hgvs_p": "p.Asp386Ala",
          "transcript": "ENST00000933602.1",
          "protein_id": "ENSP00000603661.1",
          "transcript_support_level": null,
          "aa_start": 386,
          "aa_end": null,
          "aa_length": 564,
          "cds_start": 1157,
          "cds_end": null,
          "cds_length": 1695,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000933602.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.1064A>C",
          "hgvs_p": "p.Asp355Ala",
          "transcript": "NM_001352051.2",
          "protein_id": "NP_001338980.1",
          "transcript_support_level": null,
          "aa_start": 355,
          "aa_end": null,
          "aa_length": 533,
          "cds_start": 1064,
          "cds_end": null,
          "cds_length": 1602,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001352051.2"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.1031A>C",
          "hgvs_p": "p.Asp344Ala",
          "transcript": "ENST00000953959.1",
          "protein_id": "ENSP00000624018.1",
          "transcript_support_level": null,
          "aa_start": 344,
          "aa_end": null,
          "aa_length": 522,
          "cds_start": 1031,
          "cds_end": null,
          "cds_length": 1569,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000953959.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "A",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant",
            "splice_region_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "EML2",
          "gene_hgnc_id": 18035,
          "hgvs_c": "c.503A>C",
          "hgvs_p": "p.Asp168Ala",
          "transcript": "ENST00000590575.5",
          "protein_id": "ENSP00000464764.1",
          "transcript_support_level": 3,
          "aa_start": 168,
          "aa_end": null,
          "aa_length": 180,
          "cds_start": 503,
          "cds_end": null,
          "cds_length": 543,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000590575.5"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": 3,
          "intron_rank_end": null,
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      "dbsnp": null,
      "frequency_reference_population": null,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 0,
      "gnomad_exomes_af": null,
      "gnomad_genomes_af": null,
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      "gnomad_genomes_ac": null,
      "gnomad_exomes_homalt": null,
      "gnomad_genomes_homalt": null,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8066855669021606,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.5600000023841858,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "dbscSNV1_RF",
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      "revel_prediction": "Uncertain_significance",
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      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.2,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 7.715,
      "phylop100way_prediction": "Pathogenic",
      "spliceai_max_score": 0.01,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": 0.136730724144029,
      "dbscsnv_ada_prediction": "Benign",
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      "mitotip_prediction": null,
      "acmg_score": 3,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2,PP3",
      "acmg_by_gene": [
        {
          "score": 3,
          "benign_score": 0,
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          "criteria": [
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            "PP3"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001193268.3",
          "gene_symbol": "EML2",
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          "effects": [
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          "inheritance_mode": "AR",
          "hgvs_c": "c.2015A>C",
          "hgvs_p": "p.Asp672Ala"
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      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}