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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-45771011-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=45771011&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 45771011,
      "ref": "G",
      "alt": "A",
      "effect": "stop_gained",
      "transcript": "NM_001424163.1",
      "consequences": [
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1693C>T",
          "hgvs_p": "p.Gln565*",
          "transcript": "ENST00000343373.10",
          "protein_id": "ENSP00000345997.4",
          "transcript_support_level": 1,
          "aa_start": 565,
          "aa_end": null,
          "aa_length": 630,
          "cds_start": 1693,
          "cds_end": null,
          "cds_length": 1893,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000343373.10"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1697C>T",
          "hgvs_p": "p.Pro566Leu",
          "transcript": "NM_004409.5",
          "protein_id": "NP_004400.4",
          "transcript_support_level": null,
          "aa_start": 566,
          "aa_end": null,
          "aa_length": 629,
          "cds_start": 1697,
          "cds_end": null,
          "cds_length": 1890,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000291270.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_004409.5"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1697C>T",
          "hgvs_p": "p.Pro566Leu",
          "transcript": "ENST00000291270.9",
          "protein_id": "ENSP00000291270.4",
          "transcript_support_level": 5,
          "aa_start": 566,
          "aa_end": null,
          "aa_length": 629,
          "cds_start": 1697,
          "cds_end": null,
          "cds_length": 1890,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_004409.5",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000291270.9"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1682C>T",
          "hgvs_p": "p.Pro561Leu",
          "transcript": "ENST00000447742.6",
          "protein_id": "ENSP00000413417.1",
          "transcript_support_level": 1,
          "aa_start": 561,
          "aa_end": null,
          "aa_length": 624,
          "cds_start": 1682,
          "cds_end": null,
          "cds_length": 1875,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000447742.6"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1771C>T",
          "hgvs_p": "p.Gln591*",
          "transcript": "NM_001424163.1",
          "protein_id": "NP_001411092.1",
          "transcript_support_level": null,
          "aa_start": 591,
          "aa_end": null,
          "aa_length": 656,
          "cds_start": 1771,
          "cds_end": null,
          "cds_length": 1971,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001424163.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1693C>T",
          "hgvs_p": "p.Gln565*",
          "transcript": "NM_001424162.1",
          "protein_id": "NP_001411091.1",
          "transcript_support_level": null,
          "aa_start": 565,
          "aa_end": null,
          "aa_length": 630,
          "cds_start": 1693,
          "cds_end": null,
          "cds_length": 1893,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001424162.1"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1678C>T",
          "hgvs_p": "p.Gln560*",
          "transcript": "NM_001081562.3",
          "protein_id": "NP_001075031.1",
          "transcript_support_level": null,
          "aa_start": 560,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 1678,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001081562.3"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1678C>T",
          "hgvs_p": "p.Gln560*",
          "transcript": "ENST00000458663.6",
          "protein_id": "ENSP00000401753.1",
          "transcript_support_level": 5,
          "aa_start": 560,
          "aa_end": null,
          "aa_length": 625,
          "cds_start": 1678,
          "cds_end": null,
          "cds_length": 1878,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000458663.6"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 12,
          "exon_rank_end": null,
          "exon_count": 13,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1426C>T",
          "hgvs_p": "p.Gln476*",
          "transcript": "NM_001288765.2",
          "protein_id": "NP_001275694.1",
          "transcript_support_level": null,
          "aa_start": 476,
          "aa_end": null,
          "aa_length": 541,
          "cds_start": 1426,
          "cds_end": null,
          "cds_length": 1626,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001288765.2"
        },
        {
          "aa_ref": "Q",
          "aa_alt": "*",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "stop_gained"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1252C>T",
          "hgvs_p": "p.Gln418*",
          "transcript": "NM_001424166.1",
          "protein_id": "NP_001411095.1",
          "transcript_support_level": null,
          "aa_start": 418,
          "aa_end": null,
          "aa_length": 483,
          "cds_start": 1252,
          "cds_end": null,
          "cds_length": 1452,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001424166.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1775C>T",
          "hgvs_p": "p.Pro592Leu",
          "transcript": "NM_001288764.2",
          "protein_id": "NP_001275693.1",
          "transcript_support_level": null,
          "aa_start": 592,
          "aa_end": null,
          "aa_length": 655,
          "cds_start": 1775,
          "cds_end": null,
          "cds_length": 1968,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001288764.2"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 15,
          "exon_rank_end": null,
          "exon_count": 16,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1730C>T",
          "hgvs_p": "p.Pro577Leu",
          "transcript": "ENST00000902152.1",
          "protein_id": "ENSP00000572211.1",
          "transcript_support_level": null,
          "aa_start": 577,
          "aa_end": null,
          "aa_length": 640,
          "cds_start": 1730,
          "cds_end": null,
          "cds_length": 1923,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902152.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1727C>T",
          "hgvs_p": "p.Pro576Leu",
          "transcript": "NM_001081563.3",
          "protein_id": "NP_001075032.1",
          "transcript_support_level": null,
          "aa_start": 576,
          "aa_end": null,
          "aa_length": 639,
          "cds_start": 1727,
          "cds_end": null,
          "cds_length": 1920,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001081563.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1691C>T",
          "hgvs_p": "p.Pro564Leu",
          "transcript": "ENST00000902147.1",
          "protein_id": "ENSP00000572206.1",
          "transcript_support_level": null,
          "aa_start": 564,
          "aa_end": null,
          "aa_length": 627,
          "cds_start": 1691,
          "cds_end": null,
          "cds_length": 1884,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902147.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1682C>T",
          "hgvs_p": "p.Pro561Leu",
          "transcript": "NM_001081560.3",
          "protein_id": "NP_001075029.1",
          "transcript_support_level": null,
          "aa_start": 561,
          "aa_end": null,
          "aa_length": 624,
          "cds_start": 1682,
          "cds_end": null,
          "cds_length": 1875,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001081560.3"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1676C>T",
          "hgvs_p": "p.Pro559Leu",
          "transcript": "ENST00000947414.1",
          "protein_id": "ENSP00000617473.1",
          "transcript_support_level": null,
          "aa_start": 559,
          "aa_end": null,
          "aa_length": 622,
          "cds_start": 1676,
          "cds_end": null,
          "cds_length": 1869,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000947414.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1646C>T",
          "hgvs_p": "p.Pro549Leu",
          "transcript": "ENST00000902150.1",
          "protein_id": "ENSP00000572209.1",
          "transcript_support_level": null,
          "aa_start": 549,
          "aa_end": null,
          "aa_length": 612,
          "cds_start": 1646,
          "cds_end": null,
          "cds_length": 1839,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000902150.1"
        },
        {
          "aa_ref": "P",
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          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 13,
          "exon_rank_end": null,
          "exon_count": 14,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1631C>T",
          "hgvs_p": "p.Pro544Leu",
          "transcript": "ENST00000902149.1",
          "protein_id": "ENSP00000572208.1",
          "transcript_support_level": null,
          "aa_start": 544,
          "aa_end": null,
          "aa_length": 607,
          "cds_start": 1631,
          "cds_end": null,
          "cds_length": 1824,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902149.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1547C>T",
          "hgvs_p": "p.Pro516Leu",
          "transcript": "ENST00000902148.1",
          "protein_id": "ENSP00000572207.1",
          "transcript_support_level": null,
          "aa_start": 516,
          "aa_end": null,
          "aa_length": 579,
          "cds_start": 1547,
          "cds_end": null,
          "cds_length": 1740,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000902148.1"
        },
        {
          "aa_ref": "P",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 14,
          "exon_rank_end": null,
          "exon_count": 15,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "DMPK",
          "gene_hgnc_id": 2933,
          "hgvs_c": "c.1532C>T",
          "hgvs_p": "p.Pro511Leu",
          "transcript": "ENST00000902151.1",
          "protein_id": "ENSP00000572210.1",
          "transcript_support_level": null,
          "aa_start": 511,
          "aa_end": null,
          "aa_length": 574,
          "cds_start": 1532,
          "cds_end": null,
          "cds_length": 1725,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
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      "dbsnp": "rs536277584",
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      "allele_count_reference_population": 2,
      "gnomad_exomes_af": 7.56154e-7,
      "gnomad_genomes_af": 0.0000065684,
      "gnomad_exomes_ac": 1,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.33691710233688354,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0.019999999552965164,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.243,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1095,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.19,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 4.067,
      "phylop100way_prediction": "Uncertain_significance",
      "spliceai_max_score": 0.02,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 2,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "PM2",
      "acmg_by_gene": [
        {
          "score": 2,
          "benign_score": 0,
          "pathogenic_score": 2,
          "criteria": [
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          ],
          "verdict": "Uncertain_significance",
          "transcript": "NM_001424163.1",
          "gene_symbol": "DMPK",
          "hgnc_id": 2933,
          "effects": [
            "stop_gained"
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          "inheritance_mode": "AD",
          "hgvs_c": "c.1771C>T",
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        {
          "score": 1,
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          "criteria": [
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            "BP4"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000586251.5",
          "gene_symbol": "DM1-AS",
          "hgnc_id": 53125,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.30G>A",
          "hgvs_p": null
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      ],
      "clinvar_disease": "Inborn genetic diseases,Myotonic dystrophy",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, multiple submitters, no conflicts",
      "clinvar_submissions_summary": "US:2",
      "phenotype_combined": "Myotonic dystrophy|Inborn genetic diseases",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}