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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-46755685-G-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=46755685&ref=G&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 46755685,
      "ref": "G",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "ENST00000318584.10",
      "consequences": [
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FKRP",
          "gene_hgnc_id": 17997,
          "hgvs_c": "c.235G>A",
          "hgvs_p": "p.Val79Met",
          "transcript": "NM_024301.5",
          "protein_id": "NP_077277.1",
          "transcript_support_level": null,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 495,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 1488,
          "cdna_start": 521,
          "cdna_end": null,
          "cdna_length": 3411,
          "mane_select": "ENST00000318584.10",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FKRP",
          "gene_hgnc_id": 17997,
          "hgvs_c": "c.235G>A",
          "hgvs_p": "p.Val79Met",
          "transcript": "ENST00000318584.10",
          "protein_id": "ENSP00000326570.4",
          "transcript_support_level": 1,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 495,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 1488,
          "cdna_start": 521,
          "cdna_end": null,
          "cdna_length": 3411,
          "mane_select": "NM_024301.5",
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "5_prime_UTR_premature_start_codon_gain_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FKRP",
          "gene_hgnc_id": 17997,
          "hgvs_c": "c.-195G>A",
          "hgvs_p": null,
          "transcript": "ENST00000594467.5",
          "protein_id": "ENSP00000471971.1",
          "transcript_support_level": 4,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 34,
          "cds_start": -4,
          "cds_end": null,
          "cds_length": 105,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": 545,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FKRP",
          "gene_hgnc_id": 17997,
          "hgvs_c": "c.235G>A",
          "hgvs_p": "p.Val79Met",
          "transcript": "NM_001039885.3",
          "protein_id": "NP_001034974.1",
          "transcript_support_level": null,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 495,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 1488,
          "cdna_start": 573,
          "cdna_end": null,
          "cdna_length": 3463,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FKRP",
          "gene_hgnc_id": 17997,
          "hgvs_c": "c.235G>A",
          "hgvs_p": "p.Val79Met",
          "transcript": "ENST00000391909.7",
          "protein_id": "ENSP00000375776.2",
          "transcript_support_level": 2,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 495,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 1488,
          "cdna_start": 573,
          "cdna_end": null,
          "cdna_length": 2801,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FKRP",
          "gene_hgnc_id": 17997,
          "hgvs_c": "c.235G>A",
          "hgvs_p": "p.Val79Met",
          "transcript": "ENST00000601299.5",
          "protein_id": "ENSP00000470103.1",
          "transcript_support_level": 4,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 161,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 487,
          "cdna_start": 381,
          "cdna_end": null,
          "cdna_length": 633,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FKRP",
          "gene_hgnc_id": 17997,
          "hgvs_c": "c.235G>A",
          "hgvs_p": "p.Val79Met",
          "transcript": "ENST00000593902.1",
          "protein_id": "ENSP00000470901.1",
          "transcript_support_level": 4,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 136,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 411,
          "cdna_start": 401,
          "cdna_end": null,
          "cdna_length": 577,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FKRP",
          "gene_hgnc_id": 17997,
          "hgvs_c": "c.235G>A",
          "hgvs_p": "p.Val79Met",
          "transcript": "ENST00000600005.5",
          "protein_id": "ENSP00000470335.1",
          "transcript_support_level": 4,
          "aa_start": 79,
          "aa_end": null,
          "aa_length": 113,
          "cds_start": 235,
          "cds_end": null,
          "cds_length": 342,
          "cdna_start": 441,
          "cdna_end": null,
          "cdna_length": 548,
          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
          "aa_ref": "V",
          "aa_alt": "M",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
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          "gene_symbol": "FKRP",
          "gene_hgnc_id": 17997,
          "hgvs_c": "c.235G>A",
          "hgvs_p": "p.Val79Met",
          "transcript": "ENST00000600227.5",
          "protein_id": "ENSP00000468825.1",
          "transcript_support_level": 4,
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          "cds_end": null,
          "cds_length": 318,
          "cdna_start": 476,
          "cdna_end": null,
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          "mane_select": null,
          "mane_plus": null,
          "biotype": null,
          "feature": null
        },
        {
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          "protein_coding": true,
          "strand": true,
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          "exon_rank": 4,
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          "exon_count": 4,
          "intron_rank": null,
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          "gene_symbol": "FKRP",
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          "hgvs_c": "c.235G>A",
          "hgvs_p": "p.Val79Met",
          "transcript": "ENST00000593800.5",
          "protein_id": "ENSP00000471209.1",
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          "aa_end": null,
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          "cds_start": 235,
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          "cdna_start": 523,
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          "cdna_length": 591,
          "mane_select": null,
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        {
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          "cdna_start": 430,
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          "feature": null
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        {
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          "hgvs_p": "p.Val79Met",
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          "biotype": null,
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        },
        {
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          "protein_coding": true,
          "strand": true,
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          "exon_count": 4,
          "intron_rank": null,
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          "gene_symbol": "FKRP",
          "gene_hgnc_id": 17997,
          "hgvs_c": "c.235G>A",
          "hgvs_p": "p.Val79Met",
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      "computational_score_selected": 0.017470866441726685,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.434,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.4506,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.08,
      "bayesdelnoaf_prediction": "Uncertain_significance",
      "phylop100way_score": 1.833,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -12,
      "acmg_classification": "Benign",
      "acmg_criteria": "PP2,BP4_Strong,BP6,BS1,BS2",
      "acmg_by_gene": [
        {
          "score": -12,
          "benign_score": 13,
          "pathogenic_score": 1,
          "criteria": [
            "PP2",
            "BP4_Strong",
            "BP6",
            "BS1",
            "BS2"
          ],
          "verdict": "Benign",
          "transcript": "ENST00000318584.10",
          "gene_symbol": "FKRP",
          "hgnc_id": 17997,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.235G>A",
          "hgvs_p": "p.Val79Met"
        }
      ],
      "clinvar_disease": "Autosomal recessive limb-girdle muscular dystrophy type 2I,Cardiovascular phenotype,Walker-Warburg congenital muscular dystrophy,not provided,not specified",
      "clinvar_classification": "Conflicting classifications of pathogenicity",
      "clinvar_review_status": "criteria provided, conflicting classifications",
      "clinvar_submissions_summary": "US:1 LB:5 B:4",
      "phenotype_combined": "Autosomal recessive limb-girdle muscular dystrophy type 2I|not specified|not provided|Walker-Warburg congenital muscular dystrophy|Cardiovascular phenotype",
      "pathogenicity_classification_combined": "Conflicting classifications of pathogenicity",
      "custom_annotations": null
    }
  ],
  "message": null
}