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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-48837369-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=48837369&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 48837369,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001438306.1",
      "consequences": [
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2260G>T",
          "hgvs_p": "p.Ala754Ser",
          "transcript": "NM_020904.3",
          "protein_id": "NP_065955.2",
          "transcript_support_level": null,
          "aa_start": 754,
          "aa_end": null,
          "aa_length": 779,
          "cds_start": 2260,
          "cds_end": null,
          "cds_length": 2340,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000263265.11",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_020904.3"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2260G>T",
          "hgvs_p": "p.Ala754Ser",
          "transcript": "ENST00000263265.11",
          "protein_id": "ENSP00000263265.5",
          "transcript_support_level": 1,
          "aa_start": 754,
          "aa_end": null,
          "aa_length": 779,
          "cds_start": 2260,
          "cds_end": null,
          "cds_length": 2340,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_020904.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000263265.11"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "3_prime_UTR_variant"
          ],
          "exon_rank": 17,
          "exon_rank_end": null,
          "exon_count": 17,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.*212G>T",
          "hgvs_p": null,
          "transcript": "ENST00000355496.9",
          "protein_id": "ENSP00000347683.4",
          "transcript_support_level": 1,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 583,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 1752,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000355496.9"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2281G>T",
          "hgvs_p": "p.Ala761Ser",
          "transcript": "ENST00000882972.1",
          "protein_id": "ENSP00000553031.1",
          "transcript_support_level": null,
          "aa_start": 761,
          "aa_end": null,
          "aa_length": 786,
          "cds_start": 2281,
          "cds_end": null,
          "cds_length": 2361,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882972.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2272G>T",
          "hgvs_p": "p.Ala758Ser",
          "transcript": "NM_001438306.1",
          "protein_id": "NP_001425235.1",
          "transcript_support_level": null,
          "aa_start": 758,
          "aa_end": null,
          "aa_length": 783,
          "cds_start": 2272,
          "cds_end": null,
          "cds_length": 2352,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001438306.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2272G>T",
          "hgvs_p": "p.Ala758Ser",
          "transcript": "ENST00000882967.1",
          "protein_id": "ENSP00000553026.1",
          "transcript_support_level": null,
          "aa_start": 758,
          "aa_end": null,
          "aa_length": 783,
          "cds_start": 2272,
          "cds_end": null,
          "cds_length": 2352,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882967.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2260G>T",
          "hgvs_p": "p.Ala754Ser",
          "transcript": "ENST00000882976.1",
          "protein_id": "ENSP00000553035.1",
          "transcript_support_level": null,
          "aa_start": 754,
          "aa_end": null,
          "aa_length": 779,
          "cds_start": 2260,
          "cds_end": null,
          "cds_length": 2340,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882976.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2257G>T",
          "hgvs_p": "p.Ala753Ser",
          "transcript": "ENST00000882977.1",
          "protein_id": "ENSP00000553036.1",
          "transcript_support_level": null,
          "aa_start": 753,
          "aa_end": null,
          "aa_length": 778,
          "cds_start": 2257,
          "cds_end": null,
          "cds_length": 2337,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882977.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 20,
          "exon_rank_end": null,
          "exon_count": 20,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2257G>T",
          "hgvs_p": "p.Ala753Ser",
          "transcript": "ENST00000956196.1",
          "protein_id": "ENSP00000626255.1",
          "transcript_support_level": null,
          "aa_start": 753,
          "aa_end": null,
          "aa_length": 778,
          "cds_start": 2257,
          "cds_end": null,
          "cds_length": 2337,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956196.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2206G>T",
          "hgvs_p": "p.Ala736Ser",
          "transcript": "ENST00000882973.1",
          "protein_id": "ENSP00000553032.1",
          "transcript_support_level": null,
          "aa_start": 736,
          "aa_end": null,
          "aa_length": 761,
          "cds_start": 2206,
          "cds_end": null,
          "cds_length": 2286,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882973.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2185G>T",
          "hgvs_p": "p.Ala729Ser",
          "transcript": "NM_001438307.1",
          "protein_id": "NP_001425236.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 754,
          "cds_start": 2185,
          "cds_end": null,
          "cds_length": 2265,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001438307.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2185G>T",
          "hgvs_p": "p.Ala729Ser",
          "transcript": "ENST00000882966.1",
          "protein_id": "ENSP00000553025.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 754,
          "cds_start": 2185,
          "cds_end": null,
          "cds_length": 2265,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882966.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2185G>T",
          "hgvs_p": "p.Ala729Ser",
          "transcript": "ENST00000956197.1",
          "protein_id": "ENSP00000626256.1",
          "transcript_support_level": null,
          "aa_start": 729,
          "aa_end": null,
          "aa_length": 754,
          "cds_start": 2185,
          "cds_end": null,
          "cds_length": 2265,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956197.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2182G>T",
          "hgvs_p": "p.Ala728Ser",
          "transcript": "ENST00000882969.1",
          "protein_id": "ENSP00000553028.1",
          "transcript_support_level": null,
          "aa_start": 728,
          "aa_end": null,
          "aa_length": 753,
          "cds_start": 2182,
          "cds_end": null,
          "cds_length": 2262,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882969.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2164G>T",
          "hgvs_p": "p.Ala722Ser",
          "transcript": "ENST00000594100.2",
          "protein_id": "ENSP00000471274.2",
          "transcript_support_level": 3,
          "aa_start": 722,
          "aa_end": null,
          "aa_length": 747,
          "cds_start": 2164,
          "cds_end": null,
          "cds_length": 2244,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000594100.2"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2152G>T",
          "hgvs_p": "p.Ala718Ser",
          "transcript": "NM_001438308.1",
          "protein_id": "NP_001425237.1",
          "transcript_support_level": null,
          "aa_start": 718,
          "aa_end": null,
          "aa_length": 743,
          "cds_start": 2152,
          "cds_end": null,
          "cds_length": 2232,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001438308.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2152G>T",
          "hgvs_p": "p.Ala718Ser",
          "transcript": "ENST00000882968.1",
          "protein_id": "ENSP00000553027.1",
          "transcript_support_level": null,
          "aa_start": 718,
          "aa_end": null,
          "aa_length": 743,
          "cds_start": 2152,
          "cds_end": null,
          "cds_length": 2232,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882968.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 19,
          "exon_rank_end": null,
          "exon_count": 19,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2149G>T",
          "hgvs_p": "p.Ala717Ser",
          "transcript": "ENST00000956195.1",
          "protein_id": "ENSP00000626254.1",
          "transcript_support_level": null,
          "aa_start": 717,
          "aa_end": null,
          "aa_length": 742,
          "cds_start": 2149,
          "cds_end": null,
          "cds_length": 2229,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000956195.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2086G>T",
          "hgvs_p": "p.Ala696Ser",
          "transcript": "ENST00000882980.1",
          "protein_id": "ENSP00000553039.1",
          "transcript_support_level": null,
          "aa_start": 696,
          "aa_end": null,
          "aa_length": 721,
          "cds_start": 2086,
          "cds_end": null,
          "cds_length": 2166,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000882980.1"
        },
        {
          "aa_ref": "A",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 18,
          "exon_rank_end": null,
          "exon_count": 18,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "PLEKHA4",
          "gene_hgnc_id": 14339,
          "hgvs_c": "c.2083G>T",
          "hgvs_p": "p.Ala695Ser",
          "transcript": "ENST00000882975.1",
          "protein_id": "ENSP00000553034.1",
          "transcript_support_level": null,
          "aa_start": 695,
          "aa_end": null,
          "aa_length": 720,
          "cds_start": 2083,
          "cds_end": null,
          "cds_length": 2163,
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      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.007,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.074,
      "alphamissense_prediction": "Benign",
      "bayesdelnoaf_score": -0.83,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": -0.929,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -4,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong,BP6_Moderate",
      "acmg_by_gene": [
        {
          "score": -4,
          "benign_score": 6,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong",
            "BP6_Moderate"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001438306.1",
          "gene_symbol": "PLEKHA4",
          "hgnc_id": 14339,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.2272G>T",
          "hgvs_p": "p.Ala758Ser"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Likely benign",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "LB:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Likely benign",
      "custom_annotations": null
    }
  ],
  "message": null
}