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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-51345292-G-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=51345292&ref=G&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 51345292,
      "ref": "G",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_001014763.1",
      "consequences": [
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.687C>A",
          "hgvs_p": "p.Asp229Glu",
          "transcript": "NM_001985.3",
          "protein_id": "NP_001976.1",
          "transcript_support_level": null,
          "aa_start": 229,
          "aa_end": null,
          "aa_length": 255,
          "cds_start": 687,
          "cds_end": null,
          "cds_length": 768,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000309244.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001985.3"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": true,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.687C>A",
          "hgvs_p": "p.Asp229Glu",
          "transcript": "ENST00000309244.9",
          "protein_id": "ENSP00000311930.3",
          "transcript_support_level": 1,
          "aa_start": 229,
          "aa_end": null,
          "aa_length": 255,
          "cds_start": 687,
          "cds_end": null,
          "cds_length": 768,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001985.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000309244.9"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.960C>A",
          "hgvs_p": "p.Asp320Glu",
          "transcript": "ENST00000354232.8",
          "protein_id": "ENSP00000346173.3",
          "transcript_support_level": 1,
          "aa_start": 320,
          "aa_end": null,
          "aa_length": 346,
          "cds_start": 960,
          "cds_end": null,
          "cds_length": 1041,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000354232.8"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.960C>A",
          "hgvs_p": "p.Asp320Glu",
          "transcript": "NM_001014763.1",
          "protein_id": "NP_001014763.1",
          "transcript_support_level": null,
          "aa_start": 320,
          "aa_end": null,
          "aa_length": 346,
          "cds_start": 960,
          "cds_end": null,
          "cds_length": 1041,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001014763.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 7,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.762C>A",
          "hgvs_p": "p.Asp254Glu",
          "transcript": "ENST00000903309.1",
          "protein_id": "ENSP00000573368.1",
          "transcript_support_level": null,
          "aa_start": 254,
          "aa_end": null,
          "aa_length": 280,
          "cds_start": 762,
          "cds_end": null,
          "cds_length": 843,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903309.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.696C>A",
          "hgvs_p": "p.Asp232Glu",
          "transcript": "ENST00000903308.1",
          "protein_id": "ENSP00000573367.1",
          "transcript_support_level": null,
          "aa_start": 232,
          "aa_end": null,
          "aa_length": 258,
          "cds_start": 696,
          "cds_end": null,
          "cds_length": 777,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903308.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.684C>A",
          "hgvs_p": "p.Asp228Glu",
          "transcript": "ENST00000903304.1",
          "protein_id": "ENSP00000573363.1",
          "transcript_support_level": null,
          "aa_start": 228,
          "aa_end": null,
          "aa_length": 254,
          "cds_start": 684,
          "cds_end": null,
          "cds_length": 765,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903304.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.678C>A",
          "hgvs_p": "p.Asp226Glu",
          "transcript": "ENST00000903307.1",
          "protein_id": "ENSP00000573366.1",
          "transcript_support_level": null,
          "aa_start": 226,
          "aa_end": null,
          "aa_length": 252,
          "cds_start": 678,
          "cds_end": null,
          "cds_length": 759,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903307.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.675C>A",
          "hgvs_p": "p.Asp225Glu",
          "transcript": "ENST00000903310.1",
          "protein_id": "ENSP00000573369.1",
          "transcript_support_level": null,
          "aa_start": 225,
          "aa_end": null,
          "aa_length": 251,
          "cds_start": 675,
          "cds_end": null,
          "cds_length": 756,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903310.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.669C>A",
          "hgvs_p": "p.Asp223Glu",
          "transcript": "ENST00000903306.1",
          "protein_id": "ENSP00000573365.1",
          "transcript_support_level": null,
          "aa_start": 223,
          "aa_end": null,
          "aa_length": 249,
          "cds_start": 669,
          "cds_end": null,
          "cds_length": 750,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903306.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.624C>A",
          "hgvs_p": "p.Asp208Glu",
          "transcript": "ENST00000903303.1",
          "protein_id": "ENSP00000573362.1",
          "transcript_support_level": null,
          "aa_start": 208,
          "aa_end": null,
          "aa_length": 234,
          "cds_start": 624,
          "cds_end": null,
          "cds_length": 705,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903303.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.594C>A",
          "hgvs_p": "p.Asp198Glu",
          "transcript": "ENST00000903302.1",
          "protein_id": "ENSP00000573361.1",
          "transcript_support_level": null,
          "aa_start": 198,
          "aa_end": null,
          "aa_length": 224,
          "cds_start": 594,
          "cds_end": null,
          "cds_length": 675,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903302.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.528C>A",
          "hgvs_p": "p.Asp176Glu",
          "transcript": "ENST00000903305.1",
          "protein_id": "ENSP00000573364.1",
          "transcript_support_level": null,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 202,
          "cds_start": 528,
          "cds_end": null,
          "cds_length": 609,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000903305.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.465C>A",
          "hgvs_p": "p.Asp155Glu",
          "transcript": "ENST00000922469.1",
          "protein_id": "ENSP00000592528.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 181,
          "cds_start": 465,
          "cds_end": null,
          "cds_length": 546,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000922469.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.528C>A",
          "hgvs_p": "p.Asp176Glu",
          "transcript": "ENST00000596253.1",
          "protein_id": "ENSP00000469628.1",
          "transcript_support_level": 3,
          "aa_start": 176,
          "aa_end": null,
          "aa_length": 177,
          "cds_start": 528,
          "cds_end": null,
          "cds_length": 535,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000596253.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 3,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.306C>A",
          "hgvs_p": "p.Asp102Glu",
          "transcript": "ENST00000970335.1",
          "protein_id": "ENSP00000640394.1",
          "transcript_support_level": null,
          "aa_start": 102,
          "aa_end": null,
          "aa_length": 128,
          "cds_start": 306,
          "cds_end": null,
          "cds_length": 387,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000970335.1"
        },
        {
          "aa_ref": "D",
          "aa_alt": "E",
          "canonical": false,
          "protein_coding": true,
          "strand": false,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "ETFB",
          "gene_hgnc_id": 3482,
          "hgvs_c": "c.576C>A",
          "hgvs_p": "p.Asp192Glu",
          "transcript": "XM_024451418.2",
          "protein_id": "XP_024307186.1",
          "transcript_support_level": null,
          "aa_start": 192,
          "aa_end": null,
          "aa_length": 218,
          "cds_start": 576,
          "cds_end": null,
          "cds_length": 657,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "XM_024451418.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "ENSG00000267984",
          "gene_hgnc_id": null,
          "hgvs_c": "n.78+46G>T",
          "hgvs_p": null,
          "transcript": "ENST00000600974.1",
          "protein_id": null,
          "transcript_support_level": 3,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000600974.1"
        }
      ],
      "gene_symbol": "ETFB",
      "gene_hgnc_id": 3482,
      "dbsnp": "rs200877363",
      "frequency_reference_population": 0.000037794533,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 61,
      "gnomad_exomes_af": 0.000038991,
      "gnomad_genomes_af": 0.0000262961,
      "gnomad_exomes_ac": 57,
      "gnomad_genomes_ac": 4,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.031211555004119873,
      "computational_prediction_selected": "Benign",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.268,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1509,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.46,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 0.409,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PM2,BP4_Strong",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001014763.1",
          "gene_symbol": "ETFB",
          "hgnc_id": 3482,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AR",
          "hgvs_c": "c.960C>A",
          "hgvs_p": "p.Asp320Glu"
        },
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PM2",
            "BP4_Strong"
          ],
          "verdict": "Likely_benign",
          "transcript": "ENST00000600974.1",
          "gene_symbol": "ENSG00000267984",
          "hgnc_id": null,
          "effects": [
            "intron_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.78+46G>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "Multiple acyl-CoA dehydrogenase deficiency",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "Multiple acyl-CoA dehydrogenase deficiency",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}