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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-58394524-C-A (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=58394524&ref=C&alt=A&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 58394524,
      "ref": "C",
      "alt": "A",
      "effect": "missense_variant",
      "transcript": "NM_001009.4",
      "consequences": [
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "NM_001009.4",
          "protein_id": "NP_001000.2",
          "transcript_support_level": null,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 204,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000196551.8",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_001009.4"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000196551.8",
          "protein_id": "ENSP00000196551.3",
          "transcript_support_level": 1,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 204,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_001009.4",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000196551.8"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 4,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000596046.1",
          "protein_id": "ENSP00000472985.1",
          "transcript_support_level": 1,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 204,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000596046.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.538C>A",
          "hgvs_p": "p.Arg180Ser",
          "transcript": "ENST00000598495.5",
          "protein_id": "ENSP00000471388.1",
          "transcript_support_level": 3,
          "aa_start": 180,
          "aa_end": null,
          "aa_length": 225,
          "cds_start": 538,
          "cds_end": null,
          "cds_length": 678,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000598495.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000851170.1",
          "protein_id": "ENSP00000521229.1",
          "transcript_support_level": null,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 213,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 642,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851170.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 6,
          "exon_rank_end": null,
          "exon_count": 7,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000601521.5",
          "protein_id": "ENSP00000470114.1",
          "transcript_support_level": 5,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 204,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000601521.5"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000851167.1",
          "protein_id": "ENSP00000521226.1",
          "transcript_support_level": null,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 204,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851167.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000851168.1",
          "protein_id": "ENSP00000521227.1",
          "transcript_support_level": null,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 204,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851168.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000851169.1",
          "protein_id": "ENSP00000521228.1",
          "transcript_support_level": null,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 204,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851169.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000851174.1",
          "protein_id": "ENSP00000521233.1",
          "transcript_support_level": null,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 204,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851174.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000912985.1",
          "protein_id": "ENSP00000583044.1",
          "transcript_support_level": null,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 204,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912985.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000912986.1",
          "protein_id": "ENSP00000583045.1",
          "transcript_support_level": null,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 204,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912986.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000912988.1",
          "protein_id": "ENSP00000583047.1",
          "transcript_support_level": null,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 204,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912988.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000951621.1",
          "protein_id": "ENSP00000621680.1",
          "transcript_support_level": null,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 204,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 615,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000951621.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
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          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
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          "exon_count": 6,
          "intron_rank": null,
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          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.463C>A",
          "hgvs_p": "p.Arg155Ser",
          "transcript": "ENST00000851172.1",
          "protein_id": "ENSP00000521231.1",
          "transcript_support_level": null,
          "aa_start": 155,
          "aa_end": null,
          "aa_length": 200,
          "cds_start": 463,
          "cds_end": null,
          "cds_length": 603,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000851172.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000596314.5",
          "protein_id": "ENSP00000471185.1",
          "transcript_support_level": 5,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 199,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 601,
          "cdna_start": null,
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          "cdna_length": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000596314.5"
        },
        {
          "aa_ref": "R",
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          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
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          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000851171.1",
          "protein_id": "ENSP00000521230.1",
          "transcript_support_level": null,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 195,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 588,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
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          "biotype": "protein_coding",
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        },
        {
          "aa_ref": "R",
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          ],
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          "intron_rank": null,
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          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000912987.1",
          "protein_id": "ENSP00000583046.1",
          "transcript_support_level": null,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 194,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 585,
          "cdna_start": null,
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          "biotype": "protein_coding",
          "feature": "ENST00000912987.1"
        },
        {
          "aa_ref": "R",
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          "protein_coding": true,
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          "intron_rank": null,
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          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.424C>A",
          "hgvs_p": "p.Arg142Ser",
          "transcript": "ENST00000912991.1",
          "protein_id": "ENSP00000583050.1",
          "transcript_support_level": null,
          "aa_start": 142,
          "aa_end": null,
          "aa_length": 187,
          "cds_start": 424,
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          "cds_length": 564,
          "cdna_start": null,
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          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912991.1"
        },
        {
          "aa_ref": "R",
          "aa_alt": "S",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 5,
          "exon_rank_end": null,
          "exon_count": 6,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "RPS5",
          "gene_hgnc_id": 10426,
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser",
          "transcript": "ENST00000912990.1",
          "protein_id": "ENSP00000583049.1",
          "transcript_support_level": null,
          "aa_start": 159,
          "aa_end": null,
          "aa_length": 185,
          "cds_start": 475,
          "cds_end": null,
          "cds_length": 558,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000912990.1"
        },
        {
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        {
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          "biotype": "retained_intron",
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        {
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          "gene_symbol": "ENSG00000294800",
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          "biotype": "pseudogene",
          "feature": "ENST00000726052.1"
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      ],
      "gene_symbol": "RPS5",
      "gene_hgnc_id": 10426,
      "dbsnp": "rs745755584",
      "frequency_reference_population": 0.0000061960095,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 10,
      "gnomad_exomes_af": 0.00000615668,
      "gnomad_genomes_af": 0.00000657402,
      "gnomad_exomes_ac": 9,
      "gnomad_genomes_ac": 1,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.8509255647659302,
      "computational_prediction_selected": "Pathogenic",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.442,
      "revel_prediction": "Uncertain_significance",
      "alphamissense_score": 0.9991,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": 0.32,
      "bayesdelnoaf_prediction": "Pathogenic",
      "phylop100way_score": 2.31,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": -2,
      "acmg_classification": "Likely_benign",
      "acmg_criteria": "PP3_Moderate,BS2",
      "acmg_by_gene": [
        {
          "score": -2,
          "benign_score": 4,
          "pathogenic_score": 2,
          "criteria": [
            "PP3_Moderate",
            "BS2"
          ],
          "verdict": "Likely_benign",
          "transcript": "NM_001009.4",
          "gene_symbol": "RPS5",
          "hgnc_id": 10426,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "AD",
          "hgvs_c": "c.475C>A",
          "hgvs_p": "p.Arg159Ser"
        },
        {
          "score": 4,
          "benign_score": 0,
          "pathogenic_score": 4,
          "criteria": [
            "PM2",
            "PP3_Moderate"
          ],
          "verdict": "Uncertain_significance",
          "transcript": "ENST00000726052.1",
          "gene_symbol": "ENSG00000294800",
          "hgnc_id": null,
          "effects": [
            "non_coding_transcript_exon_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "n.689G>T",
          "hgvs_p": null
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}