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GeneBe API Showcase

This page demonstrates how to use the GeneBe API to query variant information. The API provides programmatic access to genomic annotations and variant data.

API presented here should be used for checking single variants. If you want to check many variants at once, please use other API endpoints that you will find in the documentation.

Documentation & Advanced Usage

Complete API documentation:docs.genebe.net/docs/api/overview/

Interactive endpoint tester:api.genebe.net/cloud/gb-api-doc/swagger-ui/

Python client for pandas:pypi.org/project/genebe/

Java CLI for VCF files:github.com/pstawinski/genebe-cli

All tools documented at:docs.genebe.net

API Request Examples for Variant: 19-680361-C-T (hg38)

Bash / cURL Example

bash
curl "https://api.genebe.net/cloud/api-public/v1/variant?chr=19&pos=680361&ref=C&alt=T&genome=hg38&allGenes=true"

API Response

json
{
  "variants": [
    {
      "chr": "19",
      "pos": 680361,
      "ref": "C",
      "alt": "T",
      "effect": "missense_variant",
      "transcript": "NM_005860.3",
      "consequences": [
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FSTL3",
          "gene_hgnc_id": 3973,
          "hgvs_c": "c.377C>T",
          "hgvs_p": "p.Ser126Leu",
          "transcript": "NM_005860.3",
          "protein_id": "NP_005851.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 263,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 792,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "ENST00000166139.9",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "NM_005860.3"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": true,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 5,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FSTL3",
          "gene_hgnc_id": 3973,
          "hgvs_c": "c.377C>T",
          "hgvs_p": "p.Ser126Leu",
          "transcript": "ENST00000166139.9",
          "protein_id": "ENSP00000166139.3",
          "transcript_support_level": 1,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 263,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 792,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": "NM_005860.3",
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000166139.9"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 3,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FSTL3",
          "gene_hgnc_id": 3973,
          "hgvs_c": "c.377C>T",
          "hgvs_p": "p.Ser126Leu",
          "transcript": "ENST00000905299.1",
          "protein_id": "ENSP00000575358.1",
          "transcript_support_level": null,
          "aa_start": 126,
          "aa_end": null,
          "aa_length": 187,
          "cds_start": 377,
          "cds_end": null,
          "cds_length": 564,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000905299.1"
        },
        {
          "aa_ref": "S",
          "aa_alt": "L",
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "missense_variant"
          ],
          "exon_rank": 1,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FSTL3",
          "gene_hgnc_id": 3973,
          "hgvs_c": "c.44C>T",
          "hgvs_p": "p.Ser15Leu",
          "transcript": "ENST00000589185.2",
          "protein_id": "ENSP00000484376.1",
          "transcript_support_level": 2,
          "aa_start": 15,
          "aa_end": null,
          "aa_length": 83,
          "cds_start": 44,
          "cds_end": null,
          "cds_length": 253,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000589185.2"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": true,
          "strand": true,
          "consequences": [
            "intron_variant"
          ],
          "exon_rank": null,
          "exon_rank_end": null,
          "exon_count": 2,
          "intron_rank": 1,
          "intron_rank_end": null,
          "gene_symbol": "FSTL3",
          "gene_hgnc_id": 3973,
          "hgvs_c": "c.104-1289C>T",
          "hgvs_p": null,
          "transcript": "ENST00000964202.1",
          "protein_id": "ENSP00000634261.1",
          "transcript_support_level": null,
          "aa_start": null,
          "aa_end": null,
          "aa_length": 53,
          "cds_start": null,
          "cds_end": null,
          "cds_length": 162,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "protein_coding",
          "feature": "ENST00000964202.1"
        },
        {
          "aa_ref": null,
          "aa_alt": null,
          "canonical": false,
          "protein_coding": false,
          "strand": true,
          "consequences": [
            "non_coding_transcript_exon_variant"
          ],
          "exon_rank": 2,
          "exon_rank_end": null,
          "exon_count": 4,
          "intron_rank": null,
          "intron_rank_end": null,
          "gene_symbol": "FSTL3",
          "gene_hgnc_id": 3973,
          "hgvs_c": "n.111C>T",
          "hgvs_p": null,
          "transcript": "ENST00000592058.3",
          "protein_id": null,
          "transcript_support_level": 5,
          "aa_start": null,
          "aa_end": null,
          "aa_length": null,
          "cds_start": null,
          "cds_end": null,
          "cds_length": null,
          "cdna_start": null,
          "cdna_end": null,
          "cdna_length": null,
          "mane_select": null,
          "mane_plus": null,
          "biotype": "pseudogene",
          "feature": "ENST00000592058.3"
        }
      ],
      "gene_symbol": "FSTL3",
      "gene_hgnc_id": 3973,
      "dbsnp": "rs768914737",
      "frequency_reference_population": 0.000106981104,
      "hom_count_reference_population": 0,
      "allele_count_reference_population": 137,
      "gnomad_exomes_af": 0.000115167,
      "gnomad_genomes_af": 0.0000461121,
      "gnomad_exomes_ac": 130,
      "gnomad_genomes_ac": 7,
      "gnomad_exomes_homalt": 0,
      "gnomad_genomes_homalt": 0,
      "gnomad_mito_homoplasmic": null,
      "gnomad_mito_heteroplasmic": null,
      "computational_score_selected": 0.49944132566452026,
      "computational_prediction_selected": "Uncertain_significance",
      "computational_source_selected": "MetaRNN",
      "splice_score_selected": 0,
      "splice_prediction_selected": "Benign",
      "splice_source_selected": "max_spliceai",
      "revel_score": 0.207,
      "revel_prediction": "Benign",
      "alphamissense_score": 0.1173,
      "alphamissense_prediction": null,
      "bayesdelnoaf_score": -0.36,
      "bayesdelnoaf_prediction": "Benign",
      "phylop100way_score": 3.57,
      "phylop100way_prediction": "Benign",
      "spliceai_max_score": 0,
      "spliceai_max_prediction": "Benign",
      "dbscsnv_ada_score": null,
      "dbscsnv_ada_prediction": null,
      "apogee2_score": null,
      "apogee2_prediction": null,
      "mitotip_score": null,
      "mitotip_prediction": null,
      "acmg_score": 0,
      "acmg_classification": "Uncertain_significance",
      "acmg_criteria": "",
      "acmg_by_gene": [
        {
          "score": 0,
          "benign_score": 0,
          "pathogenic_score": 0,
          "criteria": [],
          "verdict": "Uncertain_significance",
          "transcript": "NM_005860.3",
          "gene_symbol": "FSTL3",
          "hgnc_id": 3973,
          "effects": [
            "missense_variant"
          ],
          "inheritance_mode": "",
          "hgvs_c": "c.377C>T",
          "hgvs_p": "p.Ser126Leu"
        }
      ],
      "clinvar_disease": "not specified",
      "clinvar_classification": "Uncertain significance",
      "clinvar_review_status": "criteria provided, single submitter",
      "clinvar_submissions_summary": "US:1",
      "phenotype_combined": "not specified",
      "pathogenicity_classification_combined": "Uncertain significance",
      "custom_annotations": null
    }
  ],
  "message": null
}